Atlas of Protein Spectra in the Ultraviolet and Visible Regions

Once you have seen the spectrum of one protein you have seen the spectra of all pro­ teins. Or so it would seem. While the general characteristics of the absorption curve may appear to be similar for all proteins (i. e. , in acid and neutral solution there is a minimum at 250 nm, a maximum at 278-282 nm, and no absorption above 310 nm; in alkaline solution the maximum and minimum shift to longer w...