Plant Protoplasts and Genetic Engineering I

Isolated protoplasts are a unique tool for genetic manipulation of plants. Since the discovery of a method for the enzymatic isolation of pro-· toplasts by Professor E. C. Cocking in 1960, tremendous progress has been made in this very fascinating area of research. I have witnessed the struggle in the 1960''s and early 1970''s, when obtaining a clean prepara­ tion of protoplasts was considered an ...