New and rapid advances in technology have equipped us with a variety of tools and platforms to ask fundamental questions of telomere regulation and have allowed investigators to carry out experiments using diverse model systems.
Cellular adhesion is a fundamental process that influences numerous biological activities such as morphogenesis, cell motility and division, as well as signalling.
Among the many types of DNA binding domains, C2H2 zinc finger proteins (ZFPs) have proven to be the most malleable for creating custom DNA-binding proteins.
Despite considerable variability within the scientific community, allosteric regulation can best be defined functionally as how a macromolecule binds one ligand differently when a second ligand is or is not pre-bound to the macromolecule, which constitutes a vital aspect of protein structure/function.
As a fairly new and expansive area of study, plant systems biology has been approached by scientists from fields as varied as plant physiology and astrophysics, creating a wide variety of techniques and methods to further this vital research.
The in situ hybridization and PCR technologies are now well-established molecular techniques for studying chromosomal aneuploidy and rearran- ments, gene localization and expression, and genomic organization.
This book provides a comprehensive survey of recent developments and applications of high performance capillary electrophoresis in the field of protein and peptide analysis with a distinct focus on the analysis of intact proteins.
Focusing on essential insight into the methods and techniques required to dissect the complex mechanisms of NF-[kappa]B activation, regulation, and function.
Gene expression can mean the difference between a functional and non-functional genome, between health and disease, and with the development of transgenic crops, the difference between survival and starvation.
MicroRNAs constitute a particularly important class of small RNAs given their abundance, broad phylogenetic conservation and strong regulatory effects, with plant miRNAs uniquely divulging their ancient evolutionary origins and their strong post-transcriptional regulatory effects.
This volume emphasizes new techniques to help understand protein cages and to apply them to a variety of technologies, highlighting the expertise of researchers based on three continents.
Contemporary approaches to the synthesis of chemically modified biomacromolecules (proteins, nucleic acids, lipids, and carbohydrates) not only require efficient means to control conjugation and the specific site of attachment of the conjugated moiety but also the effective use of recent developments in the fields of pharmaceutical chemistry, biomolecular/polymer engineering, and nanobiotechnology.
The regulation of intracellular Ca2+ has continued to be a powerful area of study since the publication of the first and second editions of Calcium Signaling Protocols, and the developments in the field have also, naturally, continued.
Molecular modeling has undergone a remarkable transformation in the last 20 years, as biomolecular simulation moves from the realm of specialists to the wider academic community.
With the recent completion of the sequencing of the human genome, it is widely anticipated that the number of potential new protein drugs and targets will escalate at an even greater rate than that observed in recent years.
Cryopreservation and Freeze-Drying Protocols: Second Edition is a compilation of robust, reproducible techniques for the conservation of a wide range of biological materials.
The introduction of high-performance liquid chromatography (HPLC) to the analysis of peptides and proteins some 25 years ago revolutionized the biological sciences by enabling the rapid and sensitive analysis of peptide and protein structure through the exquisite speed, sensitivity, and resolution that can be easily obtained.
Since the discovery that protein kinase C (PKC) transduces the ab- dance of signals that result in phospholipid hydrolysis, this enzyme has been at the forefront of research in signal transduction.
In recent years, much information has been revealed concerning the essential role of helicases, the enzymes that utilize the energy derived from nucleoside triphosphate hydrolysis to unwind the double stranded helical structure of nucleic acids.
Through all of the recent progress provided by high throughput DNA sequencing technologies, it has become clearer and clearer that the study of proteins and protein organelles will be the key to unlocking our ability to manipulate cells and intervene in human disease.
The study of functional glycomics requires the continuous development of rapid and sensitive methods for the identification of glycan structures and integration to structure-function relationships.
Advanced Protocols in Oxidative Stress III continues the thread of the first two books by covering technology ranging from a portable hand-held detector for remote analysis of antioxidant capacity to sophisticated technology such as shotgun lipidomics, mitochondrial imaging, nano sensors, fluorescent probes, chromatographic fingerprints, computational models and bio statistical applications.
Given the popularity and utility of Saccharomyces cerevisiae, yeast-based functional genomics and proteomics technologies, developed over the past decade, have contributed greatly to our understanding of bacterial, yeast, fly, worm and human gene functions.
Through many recent remarkable developments, perhaps the most significant advancements in the study of transcriptional regulation are the development of genome-wide approaches for measuring gene expression, exemplified by gene chips (chip), and chromatin immunoprecipitation assays (ChIP) for measuring in vivo protein-DNA interactions at any genomic loci.
The synthesis of proteins from 20 or so constituent amino acids according to a strictly defined code with an accuracy of better than 1 in 10,000 at most loca- tions is arguably the most complex task performed by cells.
This new edition presents principle methods in capillary electrophoresis (CE) separation involving CZE, MEKC, MECC, NACE, and corresponding hyphenated techniques to organic mass spectrometry and ICP-MS.
From the beginning of the OMICs biology era, science has been pursuing the reduction of the complex "e;genome-wide"e; assays in order to understand the essential biology that lies beneath it.
This handbook provides comprehensive reference information on the efficient production of secondary metabolites from plants by transgenesis and other genetic manipulation strategies.
In Flavins and Flavoproteins: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study flavins and flavoproteins.
