There is a demand for analytical methods that are able to discriminate between enantiomers in order to analyze the enantiomeric purity of compounds from natural or chemical sources not only in pharmaceutical sciences but in any field on bioactive compounds including chemistry, biology, biochemistry, forensic, and environmental sciences and many others.
Small proteins with molecular weights of <25 kDa are involved in major biological processes such as ribosome formation, stress adaption and cell cycle control.
This volume describes prominentmethodologies developed by laboratories that have been leading the field ofquantitative proteomics by mass spectrometry.
This detailed volume provides an overview of recent techniques employed in the field of G protein-coupled receptors (GPCRs) to screen for new drugs and to derive information about their receptor structure, dynamics, and function for the purpose of developing improved therapeutics.
The advent of PCR, with its power to amplify tiny amounts of DNA, quickly spawned the development of many analytical procedures that are widely used for detection, measurement, and characterization.
Featuring a diverse array of model organisms and scientific techniques, Sirtuins: Methods and Protocols collects detailed contributions from experts in the field addressing this vital family of genes.
Peptides are used ubiquitously for studies in biology, biochemistry, chemical biology, peptide based medicinal chemistry, and many other areas of research.
Research in the matrix metalloproteinase field began with the demonstration by Gross and Lapiere, in 1962, that resorbing tadpole tail expressed an enzyme that could degrade collagen gels.
Given the vital and far-reaching applications of medicinal plant metabolites worldwide, the quality and consistency of the products as well as the very survival of various species are of the utmost importance.
Immobihzatron of enzymes, cells, and organelles has expanded greatly in the past 30 years as the advantages of immobilization have been evaluated and utilized in analyttcal, biotransformation, and medical applications.
Patch Clamp Methods and Protocols surveys the typical patch clamp applications and advises scientists on identifying problems and selecting the best technique in each instance.
As the mysteries stored in our DNA have been more completely revealed, scientists have begun to face the extraordinary challenge of unraveling the int- cate network of protein-protein interactions established by that DNA fra- work.
With the advent of proteomics came the development of technologies, primarily mass spectrometry, which allowed high-throughput identification of proteins in complex mixtures.
Carbohydrate microarrays emerged as a key technology for the deciphering of the glycospace by providing a multiplex technology where tens to hundreds of carbohydrates/protein interactions can be probed in parallel.
With the development of new quantitative strategies and powerful bioinformatics tools to cope with the analysis of the large amounts of data generated in proteomics experiments, liquid chromatography with tandem mass spectrometry (LC-MS/MS) is making possible the analysis of proteins on a global scale, meaning that proteomics can now start competing with cDNA microarrays for the analysis of whole genomes.
This volume presents a comprehensive overview of all the existing methods on analysing protein arginylation, from the early methods utilizing crude protein preparations and whole-cell assays to the latest advanced methods involving recombinant protein techniques, antibodies, high precision mass spectrometry, and chemical probes.
Protein Design: Method and Applications, Second Edition expands upon the previous edition with current, detailed ideas on how to approach a potential protein design project.
The regulation of intracellular Ca2+ has continued to be a powerful area of study since the publication of the first and second editions of Calcium Signaling Protocols, and the developments in the field have also, naturally, continued.
This handbook, now in a new, second edition, is an essential resource for scientists with an interest in the role of glycosyltransferases and related genes involved in the biosynthesis of glycoproteins, glycolipids, and proteoglycans.
As the use of high-throughput screening expands and creates more interest in the academic community, the need for detailed reference materials becomes ever more pressing.
As a scientist with an interest in proteins you will, at some time in your career, isolate an enzyme that turns out to be yellow-or perhaps you already have.
Baculovirus Expression Protocols is a detailed guide for using the baculovirus expression vector system (BEVS) and/or insect cells to produce recombinant proteins.
Cell-free protein expression promises to narrow the technological gap between DNA and protein technologies and provide a platform for broad application of synthetic biology principles in the Life Sciences.
This volume provides acollection of protocols and approaches for the creation of novel ligand bindingproteins, compiled and described by many of today's leaders in the field ofprotein engineering.
From the beginning of the OMICs biology era, science has been pursuing the reduction of the complex "e;genome-wide"e; assays in order to understand the essential biology that lies beneath it.
The tissue microarray (TMA) method presents as a modern high technology, although its roots go back to the 80s when researchers frst started to combine several small pieces of tissues into so-called sausage blocks.
At the time of the lightbulb's invention, not even the most imaginative thinkers could have predicted the prevention and treatment of disease through genes delivered by electric fields.
Conceived with the intention of providing an array of strategies and technologies currently in use for glyco-engineering distinct living organisms, this book contains a wide range of methods being developed to control the composition of carbohydrates and the properties of proteins through manipulations on the production host rather than in the protein itself.
After the deciphering of the human genome and the genomes of many other organisms, the investigation of the function of gene products and their orchestral interplay is now one of the most important challenges in the life sciences.
