Biochemistry

This second edition expands upon the previous edition with current, detailed developments in the field and brings together a multi-disciplinary team of leading researchers to provide their latest protocols for clinical proteomics analysis.

This detailed book focuses on analytical separations by chromatographic and electrophoretic techniques, providing some overview along with numerous practically-oriented applications of the most important analytical techniques in chiral separation sciences.

Plant Isoprenoids: Methods and Protocols is a collection of detailed techniques that will be a useful tool for a wide range of plant biologists, as well as for scientists of other fields interested in plant isoprenoids.

Exploring the 2-D gel mapping field, the chapters in this book are separated into four different categories: Part I talks about 2-D maps reproducibility and maps modeling; Part II describes the image analysis tools that provide spot volume datasets; Part III is about the statistical methods applied to spot volume datasets to identify candidate biomarkers; and Part IV discusses differential analysis from direct image analysis tools.

This volume details established experimental protocols on the isolation, characterization, and detection of extracellular matrix (ECM) molecules as well as methods to study the activity and role of ECM components on various biological functions, the formation of exosomes, and tissue surrogates.

Receptor Tyrosine Kinases: Methods and Protocols explores on the most widely studied of the protein classes, known for their central role in several cellular functions and in a variety of human pathologies.

This detailed volume provides in-depth protocols for protein labeling techniques and applications, with an additional focus on general background information on the design and generation of the organic molecules used for the labeling step.

This book covers a wide range of state-of-the-art methodologies and detailed protocols currently used to study the actions that lipid-activated nuclear receptors and their co-regulators have in tissues and immune cell types considered classic metabolic "e;powerhouses"e;.

This volume details methods and protocols for the synthesis and characterization of glycopolymers and their biomedical applications.

This detailed volume gathers basic and advanced methods and protocols from in vitro assays and in vivo models to address the molecular and functional aspects of tau physiopathology.

This volume provides methods for modern macromolecular crystallography, including all steps leading to crystal structure determination and analysis.

This detailed volume explores protocols for studying the many facets of Ca2+-imaging, Ca2+-signaling, and Ca2+-binding along with background information on the principles and application of these techniques.

This volume introduces the reader to the field of enzyme stabilization and the different theories of enzyme stabilization, including the use of immobilization as a stabilization technique.

This detailed book explores the numerous applications of the yeast two-hybrid (Y2H) system, one of the most commonly used methods to detect protein-protein interactions.

This volume expands upon Protein Electrophoresis (2012) and provides readers with easy-to-follow and reproducible methods to study electrophoresis.

This volume is a compilation of laboratory protocols and methodology required for the study of molecular chaperones and the cellular stress response.

This detailed book expands upon the previous edition with a collection of methods for those performing experimental work on small GTPases of the Rho family.

This volume provides a detailed step-by-step description of protocols for the establishment of in vitro cultures of important medicinal plants, their mass multiplication in controlled environment, and step-wise secondary metabolite analysis.

This book provides a comprehensive survey of recent developments and applications of high performance capillary electrophoresis in the field of protein and peptide analysis with a distinct focus on the analysis of intact proteins.

This up-to-date volume includes protocols that illustrate the broad use of chromatin immunoprecipitation (ChIP) and ChIP-related methods in a variety of biological research areas.

This volume provides protocol references covering recent developments in the aptamer field.

Metabolomics is a fast growing field in systems biology and offers a powerful and promising approach for a large range of applications.

This second edition volume expands on the previous edition with updates on the latest techniques used in the field of proteomics.

This volume focuses on screening and profiling kinase inhibitors or activators.

This volume provides techniques on recent developments that use RNA scaffolds as molecular tools.

This second edition volume expands all chapters of the previous edition, which have been enhanced to cover the most recent developments, the current state of method research, and applications.

Protein Affinity Tags: Methods and Protocols provides researchers with the necessary information, tools, and strategy required for proper inquiry into a given protein's function and structure.

This volume explores the latest methods used to study and define serpin molecular structure, basic protease inhibition, serpin targets, and the roles of serpin in biology and disease using animal models.

In Flavins and Flavoproteins: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study flavins and flavoproteins.

This volume describes prominentmethodologies developed by laboratories that have been leading the field ofquantitative proteomics by mass spectrometry.

This volume covers an array of techniques available for studying SH2 domains and phosphotyrosine signaling.

This detailed volume addresses recent developments in phosphoproteomic techniques with a particular focus on the plant system.

This second edition provides up-to-date protocols focusing on techniques for the specific enrichment of phosphopeptides and phosphoproteins.

Caspases, Paracaspases, and Metacaspacses: Methods and Protocols is a collection of laboratory protocols covering current methods that are employed to measure and detect activities of these proteases in diverse biological systems, ranging from unicellular organisms to mammals.

In this volume expert researchers in the field detail the operations of microchip capillary electrophoresis.

This volume provides an overview of experimental procedures and state-of art methods to investigate FOXOs.

This volume explores the different approaches and techniques used by researchers to study the recent challenges and developments in metabolic profiling.

Cancer Genomics and Proteomics: Methods and Protocols, Second Edition includes methods for the analyses of cancer genome and proteome that have illuminated us about the changes in cancer cells.

This volume details the importance of multiple experimental techniques and computational methods needed to obtain the comprehensive picture of protein complex structure, dynamics and assembly afforded by the emerging field of integrative structural biology.

This volume explores experimental strategies to study progranulin as a regulatory protein in biological and disease processes.

This expert volume provides insights into the technological fundamentals together with a comprehensive overview of the potentialities of peptide microarray technology in basic research and clinical assays.

This volume presents a diverse collection of methodologies used to study various problems at the protein sequence and structure level.

This detailed book covers numerous expert methodologies to examine Hippo signaling on the structural, molecular, cellular, and organismal level.

This book brings together recent methods and theoretical approaches developed to dissect the activity and function of bacterial and eukaryotic Structural Maintenance of Chromosome (SMC) proteins.

This volume provides a fundamental overview of the current state of the art in natural products from marine algae, linking the complex and diverse natural resource with recent developments in extraction, analytical and bioactivity testing methodologies.

This volume provides a comprehensive study of this key method of gel-based proteomics.

This volume provides a better understanding of the advancements in phenotypic readouts and improved disease models that generate novel biological insights and recapitulate clinically relevant biology.

This detailed volume explores advances in vector design, DNA delivery, cell cultivation, host cell engineering, and bioprocess optimization within the study of recombinant protein expression in mammalian cells.

This volume provides an essential update on fundamental issues, current and new applications, as well as practical protocols to explore the extensive applications of lipases and the potential application of phospholipases.

This volume covers the latest technological advances in the characterization of the biosynthesis and functions of Rab GTPases and their regulation by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs).