Biochemistry

This detailed volume explores numerous histochemical techniques through a series of lab-tested protocols for the detection of specific molecules or metabolic processes, both at light and electron microscopy.

This book details multiple ways that soluble N-ethylmaleimide-sensitive factor attachment protein receptors( SNAREs) and their function are examined in the laboratory.

This detailed volume encompasses chapters from leadingexperts in the area of membrane proteins who describe step-by-step protocols developed these last few years to improve the functionalproduction and stabilization of recombinant integral membrane proteins (IMPs).

This third edition volume expands on the previous editions with new and improved assays that ask essential questions of telomere regulation in diverse model systems.

This detailed volume explores the continuing techniques ofstudying RNA-protein complexes and interactions as research in these areasexpand.

This volume provides a wide range of methods and protocols detailing various protein structures as platforms for building architectures with targeted application.

This volume discusses the cellular and molecular techniques used to study and characterize the different components of the CRAC channel signaling pathway.

This book describes the current techniques used to study functional disulphides and to exploit them for therapeutic outcomes.

The majority of all proteins undergo co- and/or post-translational modifications, crucially altering physical and chemical properties, folding, conformation distribution, stability, activity, and, consequently, the function of the proteins.

Conceived with the intention of providing an array of strategies and technologies currently in use for glyco-engineering distinct living organisms, this book contains a wide range of methods being developed to control the composition of carbohydrates and the properties of proteins through manipulations on the production host rather than in the protein itself.

This detailed book explores a few of the plethora of techniques and applications associated with the unique branch of science known as synthetic biology.

Advanced Protocols in Oxidative Stress III continues the thread of the first two books by covering technology ranging from a portable hand-held detector for remote analysis of antioxidant capacity to sophisticated technology such as shotgun lipidomics, mitochondrial imaging, nano sensors, fluorescent probes, chromatographic fingerprints, computational models and bio statistical applications.

This volume arranged into three sections describes biochemical, in vitro, and in vivo protocols on Semaphorins.

This volume explores numerous techniques for the genetic, molecular, biochemical, and structural examination of BCL-2 family proteins and their interactions.

This detailed volume provides an overview of recent techniques employed in the field of G protein-coupled receptors (GPCRs) to screen for new drugs and to derive information about their receptor structure, dynamics, and function for the purpose of developing improved therapeutics.

This volume presents relevantbackground information to understanding the molecular basis governingunconventional protein secretion (UPS), and in particular explores the latesttechniques and protocols that have been successfully applied for the study ofthis topic.

This updated and expanded volume reflects the current state of the structural protein field with improved and refined protocols that have been applied to particularly challenging proteins, notably integral membrane proteins and multi-protein complexes.

Thissecond edition details new emerging areas of zebrafish research focusing on geneticsand genomics, techniques for developing and analyzing zebrafish disease models,and methods for neuroscience.

The second edition covers a wide range of protein-protein interaction detection topics.

This book explores well-established and emerging conjugation strategies that are relevant for proteins used in the field of precision medicine, focusing on techniques that are suitable for antibodies, antibody-fragments such as Fabs, scFvs, or nanobodies, scaffold proteins such as FN3 or DARPin, peptides, or model proteins.

This volume provides the reader with detailed protocols that describe a variety of in vitro and in vivo techniques that study reductionist systems and human samples.

This third edition volume expands on the previous editions with updated approaches and techniques used to study protein nanotechnology and the future of nanomaterial compositions.

The book focuses on the regulation of protein function and biological activity by the post-translational modification known as acetylation.

Galectins: Methods and Protocols is the first book solely dedicated to methodological approaches designed to study galectin function.

This second edition volume expands on the previous edition with an update on the latest developments in the field and new techniques used to study secondary and supersecondary structures (SSS) in proteins.

This volume presents a comprehensive overview of all the existing methods on analysing protein arginylation, from the early methods utilizing crude protein preparations and whole-cell assays to the latest advanced methods involving recombinant protein techniques, antibodies, high precision mass spectrometry, and chemical probes.

This detailed volume examines the latest techniques and protocols associated with zymography, the practice of detecting enzyme activity on electrophoretic gels.

This volume not only discusses various common biobanking topics, it also delves into less-discussed subjects such as what is needed to start a biobank, training of new biobanking personnel, and ethnic representation in biospecimen research.

This volume explores the considerable efforts that have been directed towards the development of G Protein-Coupled Receptors (GPCR) screening assays in order to disclose GPCR acting compounds, elucidate signaling mechanisms or evaluate compound's efficacy.

This volume explores techniques used to detect lipids attached to proteins, to analyze the function of lipid modifications, and to characterize the enzymes that add and remove lipids from proteins.

This volume provides the most commonly used methods and protocols to study the apoptotic and non-apoptotic roles of CD95.

This book presents a methodological introduction on clathrin-mediated endocytosis.

Metabolic Flux Analysis: Methods and Protocols opens up the field of metabolic flux analysis to those who want to start a new flux analysis project but are overwhelmed by the complexity of the approach.

This detailed collection covers how the biological functions of histone deacetylases (HDACs) and histone acetyltransferases (HATs) can be detected in various experimental settings, both in vivo and in vitro.

This volume discusses detailed protocols for the analysis of glycosylation at the level of free glycans and glycopeptides.

This volume compiles methodologies used for detailed studies of histone variants, from their basic properties to their functional roles in chromatin and as vectors of epigenetic information.

Focusing on essential insight into the methods and techniques required to dissect the complex mechanisms of NF-[kappa]B activation, regulation, and function.

This volume explores the technological improvements in protein engineering, expression, purification, and crystallization of several rhodopsin photoactive intermediates, thus increasing our understanding of rhodopsin activation.

Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC): Methods and Protocols provides a synopsis of a large array of different SILAC methods by presenting a set of protocols that have been established by renowned scientists and their working groups.

In this volume, expert practitioners present a compilation of methods of functional data analysis (often referred to as "e;systems biology"e;) and its applications in drug discovery, medicine, and basic disease research.

This volume discusses the latest research and techniques used to study novel cellulases.

This volume collects research methodologies for both basic and translational studies of myelin authored by experts in the field.

The aim this volume is to present the methods, challenges, software, and applications of this widespread and yet still evolving and maturing field.

This volume covers a wide spectrum of techniques and approaches that are used in the upstream and downstream processing for recombinant glycoprotein production.

In this volume expert researchers detail in silico methods widely used to study peptides.

This detailed volume provides methods and techniques for detection after blotting.

This volume examines a wide array of vital technologies for advancing our understanding of the receptor-mediated actions of estrogen.

Protein Structure Prediction, Third Edition expands on previous editions by focusing on software and web servers.

This detailed volume explores state-of-the-art methods for the identification, quantification, and characterization of microbial proteins.

This volume explores three main aspects of nitric oxide (NO) research: NO treatment and detection, NO modifications, and NO detoxification.