This book provides detailed protocols and untold tips and tricks regarding the most well-known examples of proximity labeling methods, in which the protein of interest is genetically fused to or labeled with an enzyme that can generate short-lived reactive species to non-specifically label molecules within a certain radius of up to twenty nanometers.
PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis.
This volume details the current understanding of roles and regulation on histidine phosphorylation, describing methods for the characterization of protein phosphorylation on histidine.
This book provides a comprehensive survey of recent developments and applications of high performance capillary electrophoresis in the field of protein and peptide analysis with a distinct focus on the analysis of intact proteins.
This volume provides an essential update on fundamental issues, current and new applications, as well as practical protocols to explore the extensive applications of lipases and the potential application of phospholipases.
The regulation of intracellular Ca2+ has continued to be a powerful area of study since the publication of the first and second editions of Calcium Signaling Protocols, and the developments in the field have also, naturally, continued.
A comprehensive collection of robust methods for the detection of pesticide compounds or their metabolites useful in food, environmental, and biological monitoring, and in studies of exposure via food, water, air, and the skin or lungs.
The aim of this new edition is to provide detailed information on each topic and present novel ideas and views that can influence future developments in mass spectrometry-based proteomics.
This volume covers the latest technological advances in the characterization of the biosynthesis and functions of Rab GTPases and their regulation by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs).
Progress in functional proteomics has been limited for a long time, partially caused by limitations in assay sensitivity and sample capacity; however, protein microarrays have the ability to overcome these limitations so that a highly parallel analysis of hundreds of proteins in thousands of samples is attainable.
This volume details established experimental protocols on the isolation, characterization, and detection of extracellular matrix (ECM) molecules as well as methods to study the activity and role of ECM components on various biological functions, the formation of exosomes, and tissue surrogates.
Recent work has revealed that stabilizing G-quadruplexes in telomeric DNA inhibits telomerase activity, providing impetus for the development of G-quartet-interacting drugs, while G-quartet-containing oligonucleotides have been recognized as a potent class of aptamers effective against STAT3 and other transcription factors implicated in oncogenesis, proving these guanine-quartets to be a vital and rich area for future study.
This reference work provides comprehensive information about the bioactive molecules presented in our daily food and their effect on the physical and mental state of our body.
This book assembles concisely written chapters by world-leaders in the field summarizing recent advances in understanding microbial responses to hydrocarbons.
Since the first edition of Protein Nanotechnology Protocols Instruments and Applications the intersection of protein science and nanotechnology has become an exciting frontier in interdisciplinary sciences.
Although our understanding of the structure and activities of the cell nucleus and of the nanomachines which it contains is increasing rapidly, much remains to be learned.
We are in a phase of the evolution of biotechnology in which the true and potential commercial importance of carbohydrates is becoming appre- ated more fully.
This detailed volume explores techniques to study reactive oxygen species (ROS) in plants and to characterize their roles in development and stress responses.
This volume brings together a plethora of protocols and experimental methods used by scientists to study calpains, their inhibitors, and their substrates.
This detailed book focuses on analytical separations by chromatographic and electrophoretic techniques, providing some overview along with numerous practically-oriented applications of the most important analytical techniques in chiral separation sciences.
New and exciting biological functions are still being discovered for vitamin A derivatives, including the vast number of physiological activities of retinoids.
In recent years, much information has been revealed concerning the essential role of helicases, the enzymes that utilize the energy derived from nucleoside triphosphate hydrolysis to unwind the double stranded helical structure of nucleic acids.
This edited book provides knowledge about hemicelluloses biorefinery approaching production life cycle, circular economy, and valorization by obtaining value-added bioproducts and bioenergy.
This volume explores the different approaches and techniques used by researchers to study the recent challenges and developments in metabolic profiling.
This second edition volume expands on the previous edition with new information on the structural analysis of glycosaminoglycans (GAGs); chemical and enzymatic synthesis of GAGs; biophysical, biochemical, and computational analysis of GAG-protein interactions; molecular and genetic approaches to manipulating GAG cell biology; and molecular and genetic methods involving animal models and organoids.
This volume explores the recent advancements in biomolecular simulations of proteins, small molecules, and nucleic acids, with a primary focus on classical molecular dynamics (MD) simulations at atomistic, coarse-grained, and quantum/ab-initio levels.
Chemical library technologies have brought about dramatic changes in the drug discovery process, and, though still evolving, they have become an integral part of ongoing drug discovery research.
Molecular Modeling of Proteins, Second Edition provides a theoretical background of various methods available and enables non-specialists to apply methods to their problems by including updated chapters and new material not covered in the first edition.
This manual provides insights into the theory and practical aspects of several biotechnological and biochemical techniques for plants, protozoa, nematodes, insects and fishes, as well as human samples.
During the past decade as the data on gene sequences and expression patterns rapidly accumulated, cell-free protein synthesis technology has also experienced a revolution, becoming a powerful tool for the preparation of proteins for their functional and structural analysis.
This detailed volume explores techniques for researching brown adipose tissue (BAT) and the fascinating biology and therapeutic potential of thermogenic adipocytes.
This second edition volume expands on the previous edition with many new and updated chapters discussing the latest techniques used to investigate cell wall biochemistry, biomechanical properties, chemistry, and biology.
The tissue microarray (TMA) method presents as a modern high technology, although its roots go back to the 80s when researchers frst started to combine several small pieces of tissues into so-called sausage blocks.
This book collects up-to-date advanced protocols and advice from leading experts in the area of membrane protein biology that can be applied to structural and functional studies of any membrane protein system.
Ranging from the evolution of pathogenicity to oceanic carbon cycling, the many and varied roles that bacteriophages play in microbial ecology and evolution have inspired increased interest within the scientific community.
The multidisciplinary science of chemical proteomics studies how small molecules of synthetic or natural origin bind to proteins and modulate their function.
New insights into modern medicine and systems biology are enabled by innovative protocols and advanced technologies in mass spectrometry-based proteomics.
This volume focuses on protein analysis, including a wide range of the use of mass spectrometry and other protein methods within neurobiological disciplines.
This edition details a collection of specific shotgun proteomics-based laboratory techniques and applications developed in leading laboratories and proteomics units worldwide.
This volume emphasizes new techniques to help understand protein cages and to apply them to a variety of technologies, highlighting the expertise of researchers based on three continents.
