Plant Secondary Metabolites provides reliable assays that will equip researchers to meet the unprecedented challenge of fulfilling the huge demand for feed driven by increasing demand of animal protein in developing countries.
Focused on one of the most important and fascinating fields of modern biology, Exocytosis and Endocytosis, Second Edition presents a comprehensive collection of protocols for cutting edge, recently developed methods of studying exocytosis and endocytosis in vitro and in vivo.
Receptor Tyrosine Kinases: Methods and Protocols explores on the most widely studied of the protein classes, known for their central role in several cellular functions and in a variety of human pathologies.
Adenovirus Methods and Protocols, Second Edition, now in two volumes, is an essential resource for adenovirus (Ad) researchers beginning in the field, and an inspirational starting point for researchers looking to branch into new areas of Ad study.
Membrane proteins and membrane lipids form complex interactive systems that are highly dynamic and able to be studied only by combinations of different in vivo and in vitro techniques.
Tissue Morphogenesis: Methods and Protocols highlights major techniques, both experimental and computational, for the study of tissue morphogenesis, divided into several sections, with specific focus on techniques to image, manipulate, model and analyze tissue morphogenesis.
This handbook, now in a new, second edition, is an essential resource for scientists with an interest in the role of glycosyltransferases and related genes involved in the biosynthesis of glycoproteins, glycolipids, and proteoglycans.
Microarray Technology, Volumes 1 and 2, present information in designing and fabricating arrays and binding studies with biological analytes while providing the reader with a broad description of microarray technology tools and their potential applications.
One of the striking findings of modern developmental biology has been the high degree of conservation of signaling and developmental mechanisms amongst different animal species.
Human Fertility: Methods and Protocols is intended for all practitioners of reproductive medicine and ART, as well as for embryologists and reproductive, developmental, cell and molecular biologists and others in the biomedical sciences.
Many unexplored complex cellular and organismal adaptations occur in response to the stress of alcohol exposure, and its contribution to the development of chronic diseases, such as osteoporosis, heart disease and diabetes, is particularly relevant today, given the increased incidence of these diseases in our aging population.
As a high throughput method for analyzing gene function, cell-based microarrays have proven to be of vital importance, allowing high throughput analysis of over expression and knock down of proteins.
In recent years, our ability to understand and manipulate epidermal cells has increased tremendously, opening significant new possibilities in both basic science research and in regenerative medicine, including would healing and transplantation.
Almost daily, new technologies are being presented that move the field of human pluripotent stem cell research towards a future that may yield highly-effective, personalized medical treatments.
At the time of the lightbulb's invention, not even the most imaginative thinkers could have predicted the prevention and treatment of disease through genes delivered by electric fields.
In this revised and expanded second edition, Electron Microscopy: Methods and Protocols presents the newest technology in electron microscopy, while maintaining the practicality and accessibility of the acclaimed first edition.
This detailed volume encompasses new technological developments that specifically address questions related to adenosine 3',5'-monophosphate (cAMP) compartmentalization, that probe relevant protein-protein interactions, that increase the spatial and temporal resolution of cAMP signal detection, and that can facilitate integration of the mounting complexity of the information that is becoming available on this signaling system.
This volume provides a wide rangeof protocols used in studying the nuclear envelope, with special attention tothe experimental adjustments that may be required to successfully investigatethis complex organelle in cells from various organisms.
In the last few years, significant breakthroughs in transcription research expanded our appreciation for the complexity of molecular controls on gene expression in mammalian cells.
Since newly created beings are often perceived as either wholly good or bad, the genetic alteration of living cells impacts directly on a symbolic meaning deeply imbedded in every culture.
Adenovirus Methods and Protocols, Second Edition, now in two volumes, is an essential resource for adenovirus (Ad) researchers beginning in the field, and an inspirational starting point for researchers looking to branch into new areas of Ad study.
Once believed to be involved mainly with energetics, including the production of ATP, knowledge of the role of redox in the control of cellular activity has been expanded over recent years.
Colin Graham and a team of leading investigators and expert clinical scientists update the acclaimed first edition with a collection of powerful, up-to-date PCR-based methods for DNA sequencing, many suitable for human genome sequencing and mutation detection in human disease.
Cancer Genomics and Proteomics: Methods and Protocols provides a compendium of techniques and applications that will be of profound use to researchers interested in gene identification and function.
A great fascination for biologists, the study of embryo development provides indispensable information concerning the origins of the various forms and structures that make up an organism, and our ever-increasing knowledge gained through the study of plant embryology promises to lead to the development of numerous useful applications.
In Molecular Embryology: Methods and Protocols, Second Edition, expert investigators provide a comprehensive guide to the cutting-edge methods used across the dramatically growing field of vertebrate molecular embryology.
Microarray Technology, Volumes 1 and 2, present information in designing and fabricating arrays and binding studies with biological analytes while providing the reader with a broad description of microarray technology tools and their potential applications.
The ability to introduce macromolecules into animal cells, includ- ing DNA, RNA, proteins, and other bioactive compounds has facili- tated a broad range of biological studies, from biochemistry and biophysics to molecular biology, cell biology, and whole animal stud- ies.
Flow cytometry has evolved since the 1940s into a multidisciplinary field incorporating aspects of laser technology, fluid dynamics, electronics, optics, computer science, physics, chemistry, biology, and mathematics.
Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis is a comprehensive review of nuclear transfer technology in vertebrates, aimed at reprogramming differentiated nuclei and effecting targeted gene transfer.
Despite political and ethical controversies surrounding the study of human embryonic stem (hES) cells, new freedoms in regard to using them for research has allowed interest to remain high in understanding the regulatory mechanisms of stem cell self-renewal, their differentiation along various lineages, and their potential use in regenerative medicine.