Integrins play pivotal roles not only across a wide range of physiological processes including tissue morphogenesis, immune responses, wound healing, and regulation of cell growth and differentiation, but also in numerous pathological phenomena such as autoimmunity, thrombosis, and cancer metastasis/progression.
International biobank collaborations allow for studies with large number of subjects where generalizability of findings across populations can be investigated, which means establishing quality criteria concerning the nature of the sample, conditions of sample storage, and the adequacy of available information is of vital importance.
Recent stem cell research has revealed that miRNA and RNAi-mediated gene regulation is one of the vital determinates controlling the state of cell differentiation, with the small RNAs serving as key elements involved in regulatory network control of pluripotent cell fate determination.
Immunoinformatics: Predicting Immunogenicity In Silico is a primer for researchers interested in this emerging and exciting technology and provides examples in the major areas within the field of immunoinformatics.
Expert scientific and clinical investigators present proven human cell culture techniques applicable to tissue samples taken from a wide variety of organs, particularly those prone to pathological change.
The second edition of Biological Aging: Methods and Protocols expands upon the previous edition with current, detailed, useful and promising methods currently available to study aging.
Most laboratories conducting studies that use molecular biology techniques employ in vitro transcription and translation systems as a routine part of their day-to-day research.
New techniques to study cell signaling and function can develop at a staggering pace; however, many approaches are as valid today as on the day they were established.
A great fascination for biologists, the study of embryo development provides indispensable information concerning the origins of the various forms and structures that make up an organism, and our ever-increasing knowledge gained through the study of plant embryology promises to lead to the development of numerous useful applications.
Harry Baker and Rosalind Ridley have done an admirable job in assem- bling this collection of articles that describe the methodology frequently used to study a group of CNS illnesses often referred to as the "e;prion diseases.
This volume provides different methodologies for all classes of histone deacetylases, which includes detailed procedures on Class I and II histone deacetylase inhibitors, SIRT inhibitors, and bromodomain inhibitors.
PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis.
Since the discovery of p53 as a tumor suppressor, numerous methods have evolved to reveal the unique structural features and biochemical functions of this protein.
Detection and analysis of DNA damage is of critical importance in a variety of biological disciplines studying apoptosis, cell cycle and cell di- sion, carcinogenesis, tumor growth, embryogenesis and aging, neu- degenerative and heart diseases, anticancer drug development, environmental and radiobiological research, and others.
The polymerase chain reaction (PCR) is one of the most important molecular biological methods ever devised, with numerous applications to cli- cal molecular medicine.
Since the conception of this acclaimed series of volumes examining neural tissue culture, the expansion of neuroscience has continued to produce vital discoveries that utilize tissue culture methodologies.
Since the publication of the first edition, lentivirus vector-based technologies, through in vitro and in vivo gene transfer in eukaryotic animal cells, continue to offer the most promising opportunities for curing genetic disorders, as well as cancer and infectious diseases.
Tissue Morphogenesis: Methods and Protocols highlights major techniques, both experimental and computational, for the study of tissue morphogenesis, divided into several sections, with specific focus on techniques to image, manipulate, model and analyze tissue morphogenesis.
This detailed volume encompasses chapters from leadingexperts in the area of membrane proteins who describe step-by-step protocols developed these last few years to improve the functionalproduction and stabilization of recombinant integral membrane proteins (IMPs).
Nuclear Reprogramming: Methods and Protocols, Second Edition includes not only classic methods to perform nuclear transfer in different species but also several techniques to assess the early and late development of the reconstructed embryos, at the cellular, molecular, and epigenetic level.
Since the first edition of this book dedicated to differential display (DD) technology was published in 1997, we have witnessed an explosive interest in studying differential gene expression.
This volume is the result of an explosion ofmolecular-based research on Cilia, which began with the discovery of theuniversality of intraflagellar transport (IFT) and ciliary genomics/proteomics.
Given the variety of studies and data that have suggested the existence of heterogeneous populations or subpopulations of stem cells, this detailed volume examines different aspects of stem cell heterogeneity.
Cell gene engineering is emerging as a field with outstanding impact, not only in medicine/biology, but also, and perhaps most importantly, in agriculture and in all those food sciences involved in the fight against world hunger.
Mesenchymal Stem Cells have seen an unprecedented level of interest in the last decade, primarily due to their relative ease of isolation, the large numbers of cells present in the adult, and the ability to propagate these cells in culture.
Membrane proteins play key roles in numerous cellular processes, in particular mediating cell-to-cell communication and signaling events that lead to a multitude of biological effects.
Recent stem cell research has revealed that miRNA and RNAi-mediated gene regulation is one of the vital determinates controlling the state of cell differentiation, with the small RNAs serving as key elements involved in regulatory network control of pluripotent cell fate determination.
Since the publication of the first edition, the techniques and experimental approaches employed in the study of adipose tissue have undergone radical expansion and revision.
This book comprehensively reviews cell-free therapy approaches, focusing on the therapeutic potential of mesenchymal stem cell-derived extracellular vesicles (EVs) and secretomes across various reparative and regenerative medicine fields.
When setting out to decide on the content of DNA Repair Protocols: Prokaryotic Systems, I was conscious of the need to portray the vast array of pathways and enzymatic activities that are part of the discipline of DNA repair.
