Driven by methodological success in identifying reliable lineage markers, regulatory T cells have quickly been recognized as the most numerous subset of immune regulators in the body with critical functions in a wide array of immune responses.
This volume examines cell-cell interactions and stem cell renewal, two topics that are now inexorably linked as science strives to understand the stem cell niche and its function.
Flow cytometers are technically sophisticated instruments that utilize pr- ciples of physics, chemistry, engineering, digital electronics, optics, analog devices, laser technology, and biology to measure the light scattering and fluor- cent characteristics of particles.
This volume presents protocols on yeast cytokinesis that will help Molecular and Cellular Biology researchers in the use of these microorganisms to approach the study of general or specific key questions in cytokinesis.
This volume is comprised of a collection of experimental protocols for common techniques and strategies used to study the biogenesis of b-barrel outer membrane proteins in Gram-negative bacteria.
Over the past twenty years, the disciplines of cellular and molecular biology have generated a bounty of information pertaining to the understanding of the structure and function of eukaryotic cells .
Post-translational protein modifications by members of the ubiquitin family are widely recognized as important regulatory control systems for a variety of biological pathways.
Since their discovery, Wnt signaling molecules have been shown to control key events in embryogenesis, to maintain tissue homeostasis in the adult and, when aberrantly activated, to promote human degenerative diseases and cancer, thus making them a vital area of study.
Stem Cells and Tissue Repair: Methods and Protocols presents in-depth methods for the three major approaches of rejuvenating an aging or sick body: latent regenerative capacity stimulated in a targeted way, replacement organs grown de novo and surgically implanted, and tissue surgically implanted and coaxed to integrate and restore problem areas.
Microchip-Based Assay Systems explores recent progress in the microelectronics arena, the resultant miniaturization of component device features to nanometer size particles, and the ensuing growth in the development and use of microchip-based techniques in leading laboratories around the world.
Over time, it has become clear that changes in stem cells do occur during aging, not only in their number but also in their relationship to their microenvironment and their functionality as reflected in changes to their metabolome.
Cytokines in Human Health: Immunotoxicology, Pathology, and Therapeutic Applications explores the role of cytokines in immunotoxicology and human health using a variety of complex methods, from basic research to highly applied therapeutic applications.
Over the past two decades experimental studies have solidified the int- pretation of the cytoskeleton as a highly dynamic network of microtubules, actin microfilaments, intermediate filaments, and myosin filaments.
Although our understanding of the structure and activities of the cell nucleus and of the nanomachines which it contains is increasing rapidly, much remains to be learned.
By the end of the 1980s only two microtubule-dependent motors, the plus end-directed kinesin and the minus end-directed cytoplasmic dynein, had been identified.
Assisted reproductive technologies have had a profound impact on biomedical research through transgenic animals, food supply and production, as wells as genetic gain of domestic species, and treatment of human infertility.
In assembling this book, Protocols for Nucleic Acid Analysis by Nonradioactive Probes, I have endeavored to select protocols that have wide applicability.
A landmark work covering the major aspects of the science, technology and application of membrane operations and related fields, from basic phenomena to the most advanced applications and future perspectives.
This book, split into two volumes, presents a broad coverage of the principles and recent developments of sample preparation and fractionation tools in Expression Proteomics in general and for two-dimensional electrophoresis (2-DE) in particular.
Whether the question is one of basic cell survival, or whether it is being used to correlate cell number to some other factor such as matrix synthesis, an estimate of cell viability is universally required.
Since each human is genetically distinctive, responding differently to disease-causing factors as well as drugs, the field pharmacogenomics arose to develop personalized medicine, or medicine that deals with the complexity of the human body.
Molecular biology has transformed prenatal diagnosis because it permits an accurate diagnosis to be made from very small quantities of fetal material, even single cells.
In the past decade, molecular biology has been transformed from the art of cloning a single gene to a statistical science measuring and calculating properties of entire genomes.
In Developmental Biology of the Sea Urchin and Other Marine Invertebrates: Methods and Protocols, expert researchers in the field detail many of the methods which are now used to study sea urchins and other marine invertebrates in the laboratory.
The polymerase chain reaction (PCR) is one of the most important molecular biological methods ever devised, with numerous applications to cli- cal molecular medicine.
In this fully revised edition of an established classic, expert researchers and clinicians describe in step-by-step detail updated techniques for the isolation and growth of major primary cell types, such as kidney proximal tubule cells, hepatocytes, keratinocytes, and cardiomyocytes.
Animal Cell Biotechnology: Methods and Protocols, Third Edition constitutes a comprehensive manual of state-of-the-art and new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture from lab to final production.
Since its discovery, the mammalian target of rapamycin (mTOR) has been shown to regulate many critical molecular processes in eukaryotes such as metabolism, growth, survival, aging, synaptic plasticity, memory, and immunity.
Mammalian oocytes occupy a critical nexus in reproduction as they not only contribute half the genetic makeup of the embryo but also provide virtually all of the cytoplasmic building blocks required for sustaining embryogenesis.
