Embryonic stem cells (ESCs) offer an unlimited self-renewing capacity, as opposed to the limits of adult stem cells; therefore, ESCs represent an almost bottomless resource for regenerative medicine and tissue engineering approaches.
Lipid Signaling Protocols assembles in a single volume the various tools and methodologies needed by the interested investigator to unravel lipid dependent signaling and cell function.
Acting principally to control patterns of gene expression, nuclear receptors play vital roles during embryonic development and in the regulation of metabolic and reproductive functions in adult life, which proves this superfamily of ligand-activated transcription factors to be a crucial part of biological life.
For over forty years, mesenchymal stem cells (MSCs) have been scrutinized and studied, garnering much attention due to their broad therapeutic efficacy.
In recent years, our ability to understand and manipulate epidermal cells has increased tremendously, opening significant new possibilities in both basic science research and in regenerative medicine, including would healing and transplantation.
This book covers a wide range of topics such as role of microorganisms in the global carbon cycle, the impact of climate change on microbial communities, and the feedback mechanism between them.
Flow cytometers are technically sophisticated instruments that utilize pr- ciples of physics, chemistry, engineering, digital electronics, optics, analog devices, laser technology, and biology to measure the light scattering and fluor- cent characteristics of particles.
The fundamental question of how cells grow and divide has perplexed biologists since the development of the cell theory in the mid-19th century, when it was recognized by Virchow and others that "e;all cells come from cells.
Over the past two decades experimental studies have solidified the int- pretation of the cytoskeleton as a highly dynamic network of microtubules, actin microfilaments, intermediate filaments, and myosin filaments.
Now poised to evaluate the potential in modulating Wnt signaling for therapeutic agendas in cancer, wound healing, and degenerative disease, this book collects protocols from an exciting area of study.
Focusing on essential insight into the methods and techniques required to dissect the complex mechanisms of NF-[kappa]B activation, regulation, and function.
As membrane trafficking research has expanded over the past thirty years, a remarkable convergence of information has been gained by using genetic approaches in yeast cells with biochemical approaches in mammalian cells.
Providing experimental methods and protocols for performing pheromone research in a variety of organisms ranging from invertebrates to vertebrates, Pheromone Signaling: Methods and Protocols covers a wide spectrum of experimental approaches necessary for handling pheromone molecules, measuring receptor response and neural activation, and analyzing behavioral output.
Since the publication of the first edition, the techniques and experimental approaches employed in the study of adipose tissue have undergone radical expansion and revision.
New and exciting biological functions are still being discovered for vitamin A derivatives, including the vast number of physiological activities of retinoids.
The ability to detect and quantify apoptosis, to understand its biochemistry, and to identify its regulatory genes and proteins is crucial to biomedical research.
This volume containsprotocols specifically designed for studying programmed cell death, and alsodiscusses recent advances in techniques that span broader areas of biology thathave been recently used or that have potential to be incorporated into cell deathresearch.
A collection of classic, novel, and state-of-the-art methods for the study of cell migration in cultured cells, different model organisms, and specialized cells in normal development and disease.
In Molecular Embryology: Methods and Protocols, Second Edition, expert investigators provide a comprehensive guide to the cutting-edge methods used across the dramatically growing field of vertebrate molecular embryology.
In the last few years, significant breakthroughs in transcription research expanded our appreciation for the complexity of molecular controls on gene expression in mammalian cells.
Adenovirus Methods and Protocols, Second Edition, now in two volumes, is an essential resource for adenovirus (Ad) researchers beginning in the field, and an inspirational starting point for researchers looking to branch into new areas of Ad study.
Leading scientists in gene expression methodology and bioinformatics data analysis describe readily reproducible methods for measuring RNA levels in cells and tissues.
Considerable advances have taken place since the initial isolation and characterization of human embryonic stem (HES) cells; however, significant challenges remain before their potential for restoration and regeneration processes in patients can be realized.
Although embryonic stem cells currently enjoy the public limelight and show great pr- ise for cell based medical therapies, it is the adult stem cells which are responsible for the body's natural ability to fght disease, heal and recover, or fail and succumb to various maladies.
This volume focuses on detecting different cellular stresses, measuring pathological consequences within the cell, and investigating the role of cellular stresses in select diseases.
In recent years, single nucleotide polymorphisms have received increased and special attention in a rapidly developing field of personalized medicine and drug treatment.
Since the discovery of p53 as a tumor suppressor, numerous methods have evolved to reveal the unique structural features and biochemical functions of this protein.
In the decade since publication of the first edition of Crystallographic Methods and Protocols the field has seen several major developments that have both accelerated the pace of structure determination and made crystallography accessible to a broader range of investigators.
Thisvolume aims to describe acomplementary range of molecular, cell biological, and in vivo protocols used to investigatethe structure function of nuclear receptors, together with experimentalapproaches that may lead to new drugs to selectively target nuclearreceptor-associated diseases.
This book, split into two volumes, presents a broad coverage of the principles and recent developments of sample preparation and fractionation tools in Expression Proteomics in general and for two-dimensional electrophoresis (2-DE) in particular.
In Animal Models for Stem Cell Therapy: Methods and Protocols, expert researchers in the field detail disease models of hepatic, cardiovascular, neurological diseases, connective and contractile tissue.
This volume focuses on detecting different cellular stresses, measuring pathological consequences within the cell, and investigating the role of cellular stresses in select diseases.
New techniques to study cell signaling and function can develop at a staggering pace; however, many approaches are as valid today as on the day they were established.
