Cellular biology (cytology)

Neuroproteomics: Methods and Protocols presents experimental details for applying proteomics to the study of the central nervous system (CNS) and its dysfunction through trauma and disease.

Dictyostelium discoideum is a simple but fascinating eukaryotic microorg- ism, whose natural habitat is deciduous forest soil and decaying leaves, where the amoebae feed on bacteria and grow as independent single cells.

Animal Cell Biotechnology: Methods and Protocols, Third Edition constitutes a comprehensive manual of state-of-the-art and new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture from lab to final production.

Biomimetics and Stem Cells: Methods and Protocols collects a series of approaches to demonstrate the role and value of biomimetics for the better understanding of stem cell behavior and the acceleration of their application in regenerative medicine.

This detailed volume focuses on best practices and conditions for maintaining the most commonly used salamander species in the laboratory.

This book is a collection of protocols, to provide novel techniques for the study of the biology of T lymphocytes.

Cancer is a devastating disease that affects millions of people in the world.

Pulsed electric fields have been applied to living systems in vitro for a host of delivery applications since the early 1980s.

PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis.

The aim of Circadian Rhythms is to provide a resource that can be adopted by several types of users: those who are new to circadian biology, those who are already active in the field but are interested in learning new techniques and researchers who are considering moving to a new a model system or undertaking comparative studies and would like to consult protocols applied to different organisms before starting the study of new species.

This volume is the result of an explosion ofmolecular-based research on Cilia, which began with the discovery of theuniversality of intraflagellar transport (IFT) and ciliary genomics/proteomics.

This second edition expands on the first edition with new chapters describing methods for studyingcell movement, molecular components involved in chemotaxis, spatiotemporaldynamics of signaling components, and quantitative modeling, as well as severalupdated chapters from the first edition.

Protocols books specializing in measuring free radical and antioxidant biomarkers began to be published in 1998.

For many years I performed tissue culture in large scientific insti- tions that had a great deal of infrastructure.

A highly anticipated update of the previous edition, In Vitro Transcription and Translation Protocols, Second Edition, provides molecular biology laboratories with the most powerful techniques for exploiting in vitro transcription and translation systems.

Reflecting over three decades of advances, Epidermal Cells: Methods and Protocols, Third Edition underscores these advances in our understanding of epidermal biology with updated and entirely new protocols that compliment and extend the earlier edition.

In the areas of biochemistry and cell biology, characterizations of stability and molecular interactions call for a quantitative approach with a level of precision that matches the fine tuning of these interactions in a living cell.

Planar cell polarity (PCP), or the alignment of a collection of cells within a cell sheet, has proven, over time, to be vital in not only normal development but also in disease states.

Focusing on essential insight into the methods and techniques required to dissect the complex mechanisms of NF-[kappa]B activation, regulation, and function.

Due to the vital biological importance of RNA and proteins functioning together within a cell, a protocol volume describing experimental procedures to study their interactions should find a home in many laboratories.

This volume provides an up-to-date compilationof current methodological approaches utilized for the exploration of nucleolarstructure and function.

As regenerative medicine involves replacing diseased cells, tissues or organs, or repairing tissues in vivo, the manipulation of stem cells underlie its goals.

Molecular Methods: Stem Cell Transplantation presents a compendium of cutting-edge research on the molecular steps involved in hematopoietic stem cell (HSC) activation and self-renewal.

In Neuronal Cell Culture: Methods and Protocols, the latest aspects of the culture of neural cells are explored by experts in the field who also explain the practical and theoretical considerations of the techniques involved.

Cell cycle checkpoints control the fidelity and orderly progression of eukaryotic cell division.

Despite its inherent controversy, the exploration of the human embryo can unlock many of the answers to our deepest biological questions.

The field of eukaryotic DNA repair is enjoying a period of remarkable growth and discovery, fueled by technological advances in molecular bi- ogy, protein biochemistry, and genetics.

Poly (ADP-ribose) Polymerases (PARPs) are abundant and ubiquitous proteins that regulate crucial processes of the cell cycle, DNA repair, genomic stability, and transcriptional regulation.

This volume covers a broad range of procedures and protocols that discuss the steps for designing experimental setups for studying sperm, including analytical tools for diverse species.

It is now widely accepted that much of the dynamic function of cells and tissues is regulated from outside the cell by the extracellular matrix.

Forty years after the term "e;affinity chromatography"e; was introduced, this mode of chromatography remains a key tool in the armory of separation techniques that are available to separation and interaction scientists.

In both vertebrates and invertebrates, Hedgehog signaling regulates the development of the tissues and structures of the body.

Since the first edition, published in 2001, genomics research has taken great strides.

Microtubules are essential components of the cytoskeleton, and play critical roles in a variety of cell processes, including cell shaping, intracellular tracking, cell division, and cell migration.

The study of the extracellular matrix (ECM) and its diverse roles in tissue scaffolding and cellular signaling in both physiological and pathological processes has significantly expanded over the past decade.

In recent years, a new understanding of cell-penetrating peptides has emerged, helping researchers to expand beyond a number of long-held dogmas.

Immunologists today are interested in all of the diverse cell-types involved in host defense and have a deeper appreciation of the importance of innate immune mechanisms as a first line of protection against pathogens.

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Deficiencies in sperm function are usually the result of spermatogenic defects.

DNA sequencing has become increasingly efficient over the years, resulting in an enormous increase in the amount of data gen- ated.

Since the conception of this acclaimed series of volumes examining neural tissue culture, the expansion of neuroscience has continued to produce vital discoveries that utilize tissue culture methodologies.

It is fair to say that embryonic stem (ES) cells have taken their place beside the human genome project as one of the most discussed biomedical issues of the day.

Arthritis Research: Methods and Protocols is a compendium of data pertinent to the methods and protocols that have contributed to recent advances in molecular medicine in general, but to the molecular basis of rheumatic disease in particular.

Expert laboratorians noted for their optimized methods describe in step-by-step detail how to successfully carry out tissue-based laser microdissection in their own laboratory using the different systems available and to apply a wide range of sophisticated molecular technologies to the microdissected tissue samples.

Essential in biological functions like cell signaling and, when disturbed, a likely cause of disease, lipids have proven to be a vital force in cell biology.

Of the estimated 80,000 individual genes encoded by the human genome, approximately 10-20% are expressed by the average cell.