This detailed book collects standard techniques and cutting-edge methods that are frequently used by the research community studying the fungus Trichoderma reesei.
This detailed volume covers conventional MS-based "e;shotgun lipidomics"e; by which samples are introduced by infusion or loop injection, as well as LC-MS-based lipidomics, which are becoming increasingly important due to the ever-increasing demand for a complete and precise lipid analysis of the complex and diversified lipids in nature.
This detailed volume explores the NADPH oxidase family of enzymes in human physiology and genetic disease, in which early discoveries represent prime examples of the finest translational "e;from bed to bench and back"e; studies.
Fundamental to the development and vital functions of organisms, the migration of motile cells due to the detection of shallow gradients of specific chemical signals in their environments, or chemotaxis, can be clearly seen as a major force in cell biology.
Essential in biological functions like cell signaling and, when disturbed, a likely cause of disease, lipids have proven to be a vital force in cell biology.
Proteases decisively contribute to cancer development and promotion by regulating the activities of growth factors/cytokines and signaling receptors, as well as the composition of the extracellular matrix, thereby suppressing cell death pathways and activating cell survival pathways.
Essential in biological functions like cell signaling and, when disturbed, a likely cause of disease, lipids have proven to be a vital force in cell biology.
This book provides a selection of recently developed methods and protocols in bacterial glycomics to aid in bettering our understanding of the structures and functions of bacterial polysaccharides, their attachments to proteins and lipids, their role in biofilm formation, as well as their biosynthesis.
Metabolic Flux Analysis: Methods and Protocols opens up the field of metabolic flux analysis to those who want to start a new flux analysis project but are overwhelmed by the complexity of the approach.
In this second edition methods are described to measure the synthesis of lipids such as the phosphoinositides, ceramides and sphingomyelin, as well as techniques to molecularly characterize the various kinases and phosphatases that regulate the intracellular metabolism of these lipids.
Lipid Signaling Protocols assembles in a single volume the various tools and methodologies needed by the interested investigator to unravel lipid dependent signaling and cell function.
Due to their importance to cell biology, DNA topology-altering enzymes, collectively DNA topoisomerases and gyrases, have been studied thoroughly from a biochemical perspective, yet the expansion of research at the molecular and cellular levels continues to show a great deal of promise.
Few problems in protein biochemistry have proven to be as challenging and recalcitrant as the molecular description of nitrogenase, the catalyst of one of the most remarkable chemical transformations in biological systems: the nucleotide-dependent reduction of atmospheric dinitrogen to bioavailable ammonia.
Since the discovery of a collagen-degrading protease in the tadpole tail in 1962, matrix metalloproteinase research has led to the discovery of more than twenty distinct vertebrate MMPs, along with a variety of homologues from diverse organisms such as the sea urchin, plants, insects, and nematode worms.
This volume explores techniques used to detect lipids attached to proteins, to analyze the function of lipid modifications, and to characterize the enzymes that add and remove lipids from proteins.
This volume expands upon the previous edition with current, detailed protocols for investigating membranes and their component lipids in artificial membranes, cells, and in silico.
For most of industrial applications, enzymes and cells have to be immobilized, via very simple and cost-effective protocols, in order to be re-used for very long periods of time.
This second edition volume expands all chapters of the previous edition, which have been enhanced to cover the most recent developments, the current state of method research, and applications.
This volume provides new technologies on NRPSs and related carrier protein dependent synthases, including polyketide synthases (PKS) and fatty acid synthases (FAS).
This volume provides new technologies on NRPSs and related carrier protein dependent synthases, including polyketide synthases (PKS) and fatty acid synthases (FAS).
This book covers the basic and sustainable approach of nanofiltration membrane techniques along with their fabrication, characterization, separation mechanisms, and broad applications in the field of wastewater treatment.
Metalloproteins are involved in many key biological processes such as gas transport and metabolism, photosynthesis, cell respiration, the Krebs cycle and many other vital redox reactions.
This book is designed to serve as a textbook for core as well as elective courses offered to undergraduate and advanced undergraduate students enrolled in chemistry.
This updated and revised textbook presents a broad overview on topics concerning cellular electrophysiology - ranging from bioelectric phenomena recognized as far back as ancient Egypt to popular topics on the dangers of electrosmog.
This book aims to present the age-related alterations in redox signaling networks and their diagnostic biomarkers in aging cells using multidisciplinary approach.
This book comprehensively summarizes the biological mechanisms of coloration and pattern formation of animals at molecular and cellular level, offering up-to-date knowledge derived from remarkable progress in the last 10 years.
This book gathers selected studies on the industrial applications of glycoside hydrolases (GHs), presenting an updated classification of these enzymes, and discussing their structure, mechanisms, and various approaches to improve their catalytic efficiency.
The range of nanomaterial applications has expanded recently from catalysis, electronics, and filtration to therapeutics, diagnostics, agriculture, and food because of unique properties and potentials of different nanoparticles and nanomaterials.
This detailed volume presents a series of methods exploring membrane trafficking research, ranging from genetics and high-resolution imaging to in vitro biochemical and biophysical assays.
More than 100 years ago, Dr Casimir Funk suggested the existence of a family of organic substances that are essential for life and thus introduced the concept of "e;vital amines"e; as essential nutrients with a specific action, requiring only minute amount with the power to cure a specific disease.
