Nervous system development evolves from the well-orchestrated processes of neural induction, cell proliferation, differentiation, cell migration, survival, and synapse formation.
Zebrafish (Danio rerio) play an integral role in biomedical research, enabling researchers to examine physiological mechanisms and pathways relevant to human pathogenesis and its therapy.
In Neuronal Cell Culture: Methods and Protocols, the latest aspects of the culture of neural cells are explored by experts in the field who also explain the practical and theoretical considerations of the techniques involved.
Despite the success of earlier Neuromethads volumes, I was initially reluctant to edit a further volume because my own - search is concerned with nonneural tissues.
The development of new photochemical tools, some synthesized by chemists and some provided by nature, is rapidly changing the way neurobiological research is performed in the modern laboratory.
Major advances in molecular biology, alternative RNA splicing, protein processing, identification of gene control elements, transgenic animals and bioinformatics open up multiple research avenues and allow for better understanding of neuropeptide production and function.
Preclinical research related to mood and anxiety disorders relies extensively upon mouse behavioral tests and models, the use of which continues to increase as a greater number of underlying susceptibility genes are discovered, new targets for medications are identified, and clinical studies reveal novel neurobiological risk factors.
Dopamine, a catecholamine transmitter, plays a number of vital physiological roles in the brain and body, and, in recent years, studies on the role of dopamine in disease have opened new avenues of research and discovery.
Laser Scanning Microscopy and Quantitative Image Analysis of Neuronal Tissue brings together contributions from research institutions around the world covering pioneering applications in laser scanning microscopy and quantitative image analysis and providing information about the power and limitations of this quickly developing field.
The cerebral cytoskeleton, considered to be the working horse in brain tissues, becomes particularly important when degenerative diseases of the nervous system are considered.
The successful previous volume on this topic provided a detailed benchwork manual for the most commonly used animal models of acute neurological injuries including cerebral ischemia, hemorrhage, vasospasm, and traumatic brain and spinal cord injuries.
In the last decade, several different optical imaging techniques, either based on various voltage or calcium dyes, or more recently on modified fluorescent or bioluminescent proteins (genetically encoded) that are sensitive to calcium, have been developed to study neuronal activity, and especially groups of neurons, with the goal of mapping and deciphering the neural code underlying major neurophysiological functions.
Dopamine, a catecholamine transmitter, plays a number of vital physiological roles in the brain and body, and, in recent years, studies on the role of dopamine in disease have opened new avenues of research and discovery.
Societal, ethical, and cost-related issues, not to mention the need for sound scientific methods, have led to new and refined methods for the evaluation of health risks associated with neurotoxic compounds, relevant and predictive of exposure, relatively inexpensive, and ideally amenable to high throughput analysis and a reduction in animal use.
Due to their vital involvement in a wide variety of housekeeping and specialized cellular functions, exocytosis and endocytosis remain among the most popular subjects in biology and biomedical sciences.
Receptor and Ion Channel Detection in the Brain provides state-of-the-art and up-to-date methodological information on molecular, neuroanatomical and functional techniques that are currently used to study neurotransmitter receptors and ion channels in the brain.
As a model organism, zebrafish (Danio rerio) offer an opportunity to perform steadfast scientific investigations in a robust and high-throughput manner.
Evolving technologies starting with tracer studies, and more recently with genomics and proteomics, have provided novel information about the molecular properties of cerebral endothelium and astrocytes; however, further studies must be done in animal models of neurological diseases and in humans to get a clearer understanding of the pathogenesis of blood-brain barrier (BBB) breakdown in nervous system diseases.
The development of treatment strategies that can help patients with spinal cord injury to regain lost functions and an improved quality of life is a major medical challenge, and experimental spinal cord research has to meet these challenges by resolving fundamental problems, establishing a basis for possible novel treatment strategies of spinal cord injury, and motivating their clinical translation.
Patch Clamp Methods and Protocols surveys the typical patch clamp applications and advises scientists on identifying problems and selecting the best technique in each instance.
The past decade has seen an extraordinary growth in research interest in neurotrophic factors, and the study of the neurotrophin family has led this activity.
In recent years, the need to develop acceptable alternatives to conventional animal testing for neurotoxicity and developmental neurotoxicity has been increasingly recognized, and much effort is being directed toward the development of alternative models, utilizing mostly mammalian cells in culture but also non-mammalian model systems.
Playing an important role in the treatment of neurological disorders, the delivery of drugs to central nervous system (CNS), both administered directly and administered systematically for targeted action, encounters a major challenge in the form of the blood-brain barrier (BBB), which limits the access of drugs to the brain substance.
Many advances in modern neuroscience are enabled by the availability of chemical tools that allow sensitive, precise, and quantitative measurements of, and control over, biological processes.
Preclinical research related to mood and anxiety disorders relies extensively upon mouse behavioral tests and models, the use of which continues to increase as a greater number of underlying susceptibility genes are discovered, new targets for medications are identified, and clinical studies reveal novel neurobiological risk factors.
Laser Scanning Microscopy and Quantitative Image Analysis of Neuronal Tissue brings together contributions from research institutions around the world covering pioneering applications in laser scanning microscopy and quantitative image analysis and providing information about the power and limitations of this quickly developing field.
This volume is directed at individuals interested in the field of neuroscience who are novices or experts in the use of transmission electron microscopy.
The need to better understand the molecular, b- chemical, and cellular processes by which a developing neuronal system unfolds has led to the development of a unique set of experimental tools and organisms.
Divided into two convenient sections, Protein Kinase Technologies collects contributions from experts in the field examining recent methodologies and techniques generally applicable to protein kinase research as well as to individual protein kinases which require special attention in neuroscience.
Neurovascular Coupling Methods brings the reader up to date with the current state-of-the-art techniques in measuring blood flow in the brain, with chapters describing different techniques or combinations of techniques, applied to specific species in either healthy or abnormal brains.
This volume contains a comprehensive compilation of chromogenic and fluorescent RNA in situ hybridization (ISH) technology in many of its various shades, forms, and applications.
To foster a better understanding of dopamine receptor functionality, this detailed volume creates an interface between updated classical methods and new emerging technologies heretofore not available to new or seasoned researchers.
to the Animal Models Volumes This and several other volumes in the Neuromethods series will describe a number of animal models of neu- psychiatric disorders.
