Since the publication of the popular first edition, genomic methods have become more accessible, allowing antibiotic researchers to probe not only the sequence of antibiotic resistance determinants but the mechanism whereby they are expressed and regulated.
For both volumes:Expert investigators describe not only the classic methods, but also the many novel techniques they have perfected for the transfer of large DNAs into the cells of both microbes and animals via large-insert recombinant DNAs.
Clostridium difficile, a major nosocomial pathogen shown to be a primary cause of antibiotic-associated disease, has emerged as a highly transmissible and frequently antibiotic-resistant organism, causing a considerable burden on health care systems worldwide.
Leading experts in enzyme manipulation describe in detail their cutting-edge techniques for the screening, evolution, production, immobilization, and application of enzymes.
Salmonella: Methods and Protocols, Second Edition expands upon the previous edition with current, detailed methods on different aspects and landmarks advancing knowledge on salmonella research.
The Right Book at the Right Time The poxviruses comprise a family of complex DNA viruses that replicate in the cytoplasm of vertebrate or invertebrate cells.
The discovery of wide-spread RNA-based regulation in bacteria has led to new evaluations of the importance of bacterial regulatory RNA in every aspect of bacterial physiology.
Identifying Helicobacter infection as the leading cause of peptic ulcer disease and gastric cancer has dramatically altered the treatment of these disease states.
This Volume presents key microscopy and imaging methods for revealing the structure and ultrastructure of environmental and experimental samples, of microbial communities and cultures, and of individual cells.
Since the first edition of Vaccinia Virus and Poxvirology: Methods and Protocols was published, a number of important events related to poxvirology have occurred, such as FDA approval of a culture-based live smallpox vaccine and the vaccination of large numbers of U.
This Volume provides protocols for the biochemical analysis of hydrocarbon- and lipid-relevant products, cell components and activities of microbes that interact with hydrophobic compounds.
Each generation in a sexually reproducing organism such as a fly or a mouse passes through the bottleneck of meiosis, which is the specialized cell division that gives rise to haploid reproductive cells (sperm, eggs, spores, etc.
Ranging from the evolution of pathogenicity to oceanic carbon cycling, the many and varied roles that bacteriophages play in microbial ecology and evolution have inspired increased interest within the scientific community.
Metabolic engineering is the practice of genetically optimizing metabolic and regulatory networks within cells to increase production and/or recovery of certain substance from cells.
This volume describes the most common laboratory procedures for isolation, identification and characterization of polioviruses used in clinical and research laboratories.
Since its early days in the 1990s, the Quorum Sensing (QS) field has grown from a few dozen laboratories, investigating the pathways, proteins, and chemicals that facilitate signaling in bacteria, to hundreds of groups that have integrated evolutionary biology, computer science, mathematics, engineering, and metagenomics to create an ever-expanding and dynamic field.
This volume aims to describe a variety of techniques that reflects the wide range of research currently performed in the field of coronavirology, and begins with an overview of current understandings of coronavirus replication and pathogenesis to introduce specialists and non-specialists to the field.
This volume opens by covering two main types of approaches widely used to determine essential genes: single-gene knockouts and transposon mutagenesis, in both prokaryotes and Candida albicans.
Due to the rising threat of tuberculosis and other mycobacterial infections, methods to study the biology of the mycobacteria and to improve diagnostic, therapeutic and preventative reagents are still very much in need.
This volume covers microbiological, clinical and patophysiological aspects of sepsis and also provides general overview chapters with every chapter discussing the real clinical impact of the discussed diagnostic approaches.
The interest of investigators across a broad spectrum of scientific dis- plines has been steadily stimulated by the field of bacterial toxin research, an area that makes use of a large variety of biological, chemical, physicochemical, and medically oriented approaches.
Different from other resources, this volume offers a broad appeal to microbiologists, immunologists, and infectious disease specialists on Autoimmune Diseases.
This Volume presents key microscopy and imaging methods for revealing the structure and ultrastructure of environmental and experimental samples, of microbial communities and cultures, and of individual cells.
Different from other resources, this volume offers a broad appeal to microbiologists, immunologists, and infectious disease specialists on Autoimmune Diseases.
Metagenomics has proven to be a powerful tool for exploring the ecology, metabolic profiling, and comparison of complex microbial communities as well as its important applications in the mining of metagenomes for genes encoding novel biocatalysts and drug molecules for bioindustries.
The polymerase chain reaction (PCR) has become an increasingly important tool in microbial diagnosis in recent years, mainly because of its rapidity, high sensitivity, and specificity.
Filamentous fungi have long been known for their ability to produce an enormous range of unusual chemical compounds known as secondary metabolites, many of which have potentially useful antibiotic or pharmacological properties.
Rhinoviruses: Methods and Protocols highlights the numerous molecular, cellular and in vivo tools now available to conduct human rhinovirus (HRV) research in an effort to increase understanding of the clinical disease caused by HRVs as well as the functions of its individual proteins and its replication.
Despite major advances in HIV treatment, many areas require more study, in order to create efficacious, potent antiretrovirals that can suppress viral load completely and durably without toxic side effects, to define unknown drug targets and fine-tune known targets, and to better understand the interplay between viral and host factors.
