Scientific equipment, experiments and techniques

This volume aims to present a large panel of techniques for the study of Plant Cell Division.

This book provides a thorough introduction to widely used techniques for the study of the intersection between developmental biology and neuroscience, an exceptional area to address and investigate impacting biological questions.

This volume provides readers with a historical foundation in standard techniques and a comprehensive update on the latest methods used in making gene-modified mice.

This detailed volume contains embryo culture techniques that should inspire embryologists to consider comparative studies.

This detailed volume provides a single, valuable reference source formethods that definitively identify and accurately quantify apoptosis.

This second edition presents methods and protocols to aid readers in the design and execution of experiments used to define critical elements associated with innate immune system function.

Animal models have traditionally played a crucial role in improving our understanding of brain pathogenesis.

Cell culture methodologies have become standard procedures in most plant laboratories.

Diverse molecular, cellular, and environmental events must all come together to allow the successful formation of secondary cancers, metastases.

New techniques to study cell signaling and function can develop at a staggering pace; however, many approaches are as valid today as on the day they were established.

In the last 20 years, research activity using the zebrafish Danio rerio has increased dramatically, due in part to the ease of breeding and raising them, their genetic tractability, embryonic accessibility, and their imaging potential.

The successful previous volume on this topic provided a detailed benchwork manual for the most commonly used animal models of acute neurological injuries including cerebral ischemia, hemorrhage, vasospasm, and traumatic brain and spinal cord injuries.

As the drug discovery process shifts more and more toward specifically targeting pathways and molecules, model systems continue to increase in importance, and the mouse, with its versatility, ease of use, and remarkable similarity to the human genome, has clearly risen to the forefront of animal model studies.

Despite political and ethical controversies surrounding the study of human embryonic stem (hES) cells, new freedoms in regard to using them for research has allowed interest to remain high in understanding the regulatory mechanisms of stem cell self-renewal, their differentiation along various lineages, and their potential use in regenerative medicine.

A great fascination for biologists, the study of embryo development provides indispensable information concerning the origins of the various forms and structures that make up an organism, and our ever-increasing knowledge gained through the study of plant embryology promises to lead to the development of numerous useful applications.

In Developmental Biology of the Sea Urchin and Other Marine Invertebrates: Methods and Protocols, expert researchers in the field detail many of the methods which are now used to study sea urchins and other marine invertebrates in the laboratory.

Movement is the way that animals interact with their environment and is under the organization and complex control of the brain and spinal cord.

Our understanding of addiction and how it is treated has advanced remarkably over the past decades, and much of the progress is related directly to animal research.

Driven by methodological success in identifying reliable lineage markers, regulatory T cells have quickly been recognized as the most numerous subset of immune regulators in the body with critical functions in a wide array of immune responses.

As increasing global population and continuing economic development ensure the need for further production and cultivation of maize, the necessity of the application of transgenic technology to this model species and crop plant grows steadily.

Since the conception of this acclaimed series of volumes examining neural tissue culture, the expansion of neuroscience has continued to produce vital discoveries that utilize tissue culture methodologies.

A comprehensive collection of diverse techniques for the molecular and cellular manipulation of human embryonic stem (hES) cells.

Animal models of schizophrenia and other major psychiatric disorders have been sought for decades, and, as a result, we are now facing new vistas on pathophysiology that could lead to novel therapeutic approaches and even hint at possible preventive strategies.

In view of the numerous failures of clinical trials aimed at improving stroke therapy, the role and potential benefit of experimentally modeling focal cerebral ischemia in rodents has been debated.

The small fruit fly, Drosophila melanogaster, has for over a century now had a large impact on biological and biomedical research; however, our knowledge of the fly brain has lagged significantly behind our understanding of other aspects of its development, physiology, and function.

At some point in their careers, virtually every scientist and technician, as well as many medical professionals, regardless of their area of specialization have a need to utilize cell culture systems.

Sphingosine-1-phosphate is a bioactive lysophospholipid which has become, in recent years, the focus of much research interest as it has widespread developmental and physio-pathological actions, controlling events within the nervous, reproductive, gastrointestinal, vascular, respiratory, and immune systems, in addition to having a prominent role in cancer, early mammalian embryogenesis, and stem cells.

As the number of sequenced genomes continues to increase, understanding the functions of newly discovered molecules will require greater efficiency and further study within the context of live cells.

Grown exponentially by the genomic revolution, the use of the rat as a model of choice for physiological studies continues in popularity and at a much greater depth of understanding.

With the loss of work days, the price of health care and payments for compensation, litigation, and malpractice, and the overwhelming cost of human suffering, chronic pain syndromes affect humanity enormously on both an economic and personal level.

Fascinating biology occurs at epithelial interfaces, whether between organism and environment or within body compartments, and many diseases inflicting huge personal and societal burdens result from dysfunction of epithelial systems, e.

In Neuronal Cell Culture: Methods and Protocols, the latest aspects of the culture of neural cells are explored by experts in the field who also explain the practical and theoretical considerations of the techniques involved.

Despite the difficulty in comparing clinic-based human tests with animal model testing, there is still great value in pursuing translational approaches, as tests and treatment strategies might be developed to improve brain function in humans suffering from neurological conditions and knowledge obtained from human behavioral studies can be used to further improve the animal models of behavioral analysis.

The growth of the field of eating disorder research has led to a vast array of empirical articles, and the development of new animal models that can be used to study these disorders continues to stimulate new research.

The successful previous volume on this topic provided a detailed benchwork manual for the most commonly used animal models of acute neurological injuries including cerebral ischemia, hemorrhage, vasospasm, and traumatic brain and spinal cord injuries.

In 2010 the global area of transgenic crops reached 148 million hectares, an 87-fold increase since 1996, making it the most rapidly adopted technology in the history of modern agriculture.

As the research has continued, it has become increasingly clear that natural killer (NK) cells are critical sentinels of the innate immune response, playing important roles in protecting the body from numerous pathogens and cancer in addition to contributing to normal pregnancy and impacting the outcomes of transplantation.

In recent years, our ability to understand and manipulate epidermal cells has increased tremendously, opening significant new possibilities in both basic science research and in regenerative medicine, including would healing and transplantation.

This book is a collection of protocols, to provide novel techniques for the study of the biology of T lymphocytes.

Animal Cell Biotechnology: Methods and Protocols, Third Edition constitutes a comprehensive manual of state-of-the-art and new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture from lab to final production.

During the 20th century Xenopus has flourished as an accessible experimental model system for studying vertebrate biology.

The ability to detect and quantify apoptosis, to understand its biochemistry, and to identify its regulatory genes and proteins is crucial to biomedical research.

Given the vital and far-reaching applications of medicinal plant metabolites worldwide, the quality and consistency of the products as well as the very survival of various species are of the utmost importance.

In light of the critical contributions of macrophages and dendritic cells to diverse inflammatory diseases and to immunity and host defense, state-of-the-art approaches to the investigation of their behavior are essential.

Before the therapeutic potential of cell replacement therapy or the development of therapeutic drugs for stimulating the body's own regenerative ability to repair cells damaged by disease and injury can be fully realized, control of stem cell fate, immuno-rejection, and limited cell sources must be overcome.

Cell death is an essential process in development, and a major contributor to a wide range of human diseases.

Although the available models, whether at the cellular, tissue, or animal level, do not exactly represent the biology of human brain tumors, animal models can offer significant insights into these tumors, providing a better understanding of biological mechanisms underlying tumor generation, growth, angiogenesis, invasion, and metastasis.

Cancer is a devastating disease that affects millions of people in the world.

To prepare synchronized cells representing different stages of the cell cycle has been a great challenge for researchers across the globe.