Scientific equipment, experiments and techniques

This book deals with the quantitative (mensural) aspects of Electron Micrography (EM) [obtained by using both, Transmission (TEM) and Scanning (SEM) types] by applying Photogrammetric techniques.

This second edition provides state-of-the-art and novel methods on antibiotic isolation and purification, identification of antimicrobial killing mechanisms, as well as methods for the analysis and detection of microbial responses and adaptation strategies.

This detailed volume provides a comprehensive collection of protocols for epigenomic research, powering our ability to analyze epigenetic modifications across the entire genome.

This book compiles the relevant information related to genome editing tools and their roles in crop improvement.

This book elucidates the tissue structure and cell composition of the organs of zebrafish at the microscopic, ultrastructural and molecular levels.

This book describes a standardized method for classifying and reporting invasive endometrial malignancies via direct endometrial sampling.

Zebrafish (Danio rerio) play an integral role in biomedical research, enabling researchers to examine physiological mechanisms and pathways relevant to human pathogenesis and its therapy.

This volume provides a collection of state-of-the-art approaches addressing key aspects of multiplexed imaging.

This book discusses the application of machine learning in genomics.

THE DEFINITIVE CHEMICAL PROCESS INDUSTRY REFERENCE--FULLY REVISEDUpdated to reflect the latest developments in operational procedures for today's sophisticated chemical technologies, Chemical Technicians' Ready Reference Handbook, Fifth Edition, remains the undisputed classic in the field.

This volume presents techniques used by researchers from all branches of biology to study daily changes at a molecular level in many physiological systems.

This detailed volume focuses on genotyping and validation in addition to information on how to produce gene edited cells and animals for research.

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The ability to detect and quantify apoptosis, to understand its biochemistry, and to identify its regulatory genes and proteins is crucial to biomedical research.

The development of treatment strategies that can help patients with spinal cord injury to regain lost functions and an improved quality of life is a major medical challenge, and experimental spinal cord research has to meet these challenges by resolving fundamental problems, establishing a basis for possible novel treatment strategies of spinal cord injury, and motivating their clinical translation.

The successful previous volume on this topic provided a detailed benchwork manual for the most commonly used animal models of acute neurological injuries including cerebral ischemia, hemorrhage, vasospasm, and traumatic brain and spinal cord injuries.

This detailed book presents a technical overview and practical methodology of a variety of antibody array formats and technologies.

This volume provides readers with a historical foundation in standard techniques and a comprehensive update on the latest methods used in making gene-modified mice.

This book explores in depth a wide range of functional biomaterials-based systems for drug, gene delivery, and biomedical aspects.

This volume is essential for geneticists, molecular biologists, biochemists, and medical doctors interested in the use of mouse models in cancer research.

This atlas offers stunning color electron scanning micrographs and exceptional light microscopy pictures of capillaries, vessels and diverse histomorphological tissues and organs of Xenopus laevis.

The aim of this volume is to provide a step-by-step guide for implementing a selection of novel techniques in the lab.

A collection of new reviews and protocols from leading experts in cell cycle regulation, Cell Cycle Control: Mechanisms and Protocols, Second Edition presents a comprehensive guide to recent technical and theoretical advancements in the field.

Cell culture methodologies have become standard procedures in most plant laboratories.

This book serves as a brief introduction to phylogenetic trees and molecular evolution for biologists and biology students.

In light of the critical contributions of macrophages and dendritic cells to diverse inflammatory diseases and to immunity and host defense, state-of-the-art approaches to the investigation of their behavior are essential.

This manual provides insights into the theory and practical aspects of several biotechnological and biochemical techniques for plants, protozoa, nematodes, insects and fishes, as well as human samples.

Sphingosine-1-phosphate is a bioactive lysophospholipid which has become, in recent years, the focus of much research interest as it has widespread developmental and physio-pathological actions, controlling events within the nervous, reproductive, gastrointestinal, vascular, respiratory, and immune systems, in addition to having a prominent role in cancer, early mammalian embryogenesis, and stem cells.

Human cell culture is not a new topic, but the development of new molecular techniques and reagents which can be used to investigate cell function and the responsible intracellular mechanisms make it a continuing requirement.

This second edition presents methods and protocols to aid readers in the design and execution of experiments used to define critical elements associated with innate immune system function.

Given the vital and far-reaching applications of medicinal plant metabolites worldwide, the quality and consistency of the products as well as the very survival of various species are of the utmost importance.

This laboratory manual, published in cooperation with the International Society for Transgenic Technology (ISTT), provides almost all current methods that can be applied to the creation and analysis of genetically modified animals.

In the last 20 years, research activity using the zebrafish Danio rerio has increased dramatically, due in part to the ease of breeding and raising them, their genetic tractability, embryonic accessibility, and their imaging potential.

The small fruit fly, Drosophila melanogaster, has for over a century now had a large impact on biological and biomedical research; however, our knowledge of the fly brain has lagged significantly behind our understanding of other aspects of its development, physiology, and function.

This meticulous book explores the leading methodologies, techniques, and tools for microarray data analysis, given the difficulty of harnessing the enormous amount of data.

This updated book covers a wide range of techniques on RNA extraction, detection, quantification, visualization, and genome-wide profiling, from conventional methods to state-of-the-art high throughput approaches.

Receptor and Ion Channel Detection in the Brain provides state-of-the-art and up-to-date methodological information on molecular, neuroanatomical and functional techniques that are currently used to study neurotransmitter receptors and ion channels in the brain.

Cultured cells have combined accessibility and the ability to expand a homogeneous cell population from a relatively limited source, thus opening up a wealth of possibilities for researchers.

With many recent advances, cancer cell culture research is more important than ever before.

This volume provides current up-to-date protocols for preparing the ovary for various imaging techniques, genetic protocols for generating mutant clones, mosaic analysis and assessing cell death.

This volume details methods on several aspects of circadian research.

As the presence of genetically modified animal models in research laboratories has multiplied, the role of genetic factors in the pathogenesis of brain disorders has become particularly important.

This detailed second edition presents a wide variety of marine invertebrate model systems, from cephalochordata to holothurians, along with novel experimental protocols for taking advantage of their unique properties.

This volume provides clear and direct protocols to implement automated Design-Build-Test-Learn (DBTL) into synthetic biology research.

This detailed volume explores perspectives and methods using cell-free expression (CFE) to enable next-generation synthetic biology applications.

Almost daily, new technologies are being presented that move the field of human pluripotent stem cell research towards a future that may yield highly-effective, personalized medical treatments.

Driven by methodological success in identifying reliable lineage markers, regulatory T cells have quickly been recognized as the most numerous subset of immune regulators in the body with critical functions in a wide array of immune responses.

This book is the first comprehensive compilation of existing knowledge on the impact of cisgenic crops on biodiversity, environment, and ecosystem.

The 3rd edition of this textbook offers clear explanations of optical spectroscopic phenomena and shows how spectroscopic techniques are used in modern chemistry, biochemistry and biophysics.