This second edition presents methods and protocols to aid readers in the design and execution of experiments used to define critical elements associated with innate immune system function.
Given the vital and far-reaching applications of medicinal plant metabolites worldwide, the quality and consistency of the products as well as the very survival of various species are of the utmost importance.
This laboratory manual, published in cooperation with the International Society for Transgenic Technology (ISTT), provides almost all current methods that can be applied to the creation and analysis of genetically modified animals.
In the last 20 years, research activity using the zebrafish Danio rerio has increased dramatically, due in part to the ease of breeding and raising them, their genetic tractability, embryonic accessibility, and their imaging potential.
The small fruit fly, Drosophila melanogaster, has for over a century now had a large impact on biological and biomedical research; however, our knowledge of the fly brain has lagged significantly behind our understanding of other aspects of its development, physiology, and function.
This meticulous book explores the leading methodologies, techniques, and tools for microarray data analysis, given the difficulty of harnessing the enormous amount of data.
This updated book covers a wide range of techniques on RNA extraction, detection, quantification, visualization, and genome-wide profiling, from conventional methods to state-of-the-art high throughput approaches.
Receptor and Ion Channel Detection in the Brain provides state-of-the-art and up-to-date methodological information on molecular, neuroanatomical and functional techniques that are currently used to study neurotransmitter receptors and ion channels in the brain.
Cultured cells have combined accessibility and the ability to expand a homogeneous cell population from a relatively limited source, thus opening up a wealth of possibilities for researchers.
This volume provides current up-to-date protocols for preparing the ovary for various imaging techniques, genetic protocols for generating mutant clones, mosaic analysis and assessing cell death.
As the presence of genetically modified animal models in research laboratories has multiplied, the role of genetic factors in the pathogenesis of brain disorders has become particularly important.
This detailed second edition presents a wide variety of marine invertebrate model systems, from cephalochordata to holothurians, along with novel experimental protocols for taking advantage of their unique properties.
Almost daily, new technologies are being presented that move the field of human pluripotent stem cell research towards a future that may yield highly-effective, personalized medical treatments.
Driven by methodological success in identifying reliable lineage markers, regulatory T cells have quickly been recognized as the most numerous subset of immune regulators in the body with critical functions in a wide array of immune responses.
The 3rd edition of this textbook offers clear explanations of optical spectroscopic phenomena and shows how spectroscopic techniques are used in modern chemistry, biochemistry and biophysics.
Mitosis: Methods and Protocols provides state-of-the-art overviews on the most important approaches currently used in mitosis research spanning from the analysis of single molecules in isolation to their utilization within the complex environment of the cell.
Science Sifting is designed primarily as a textbook for students interested in research and as a general reference book for existing career scientists.
Understanding the molecular and cellular mechanisms underlying the development of specific neural circuits is not just an intellectual curiosity but also central to our ability to develop therapeutic approaches to repair damaged pathways in the future.
This book comprehensively covers in an easy-to-understand language, the principles and working of advanced analytical techniques used in Dairy Chemistry research.
As the research has continued, it has become increasingly clear that natural killer (NK) cells are critical sentinels of the innate immune response, playing important roles in protecting the body from numerous pathogens and cancer in addition to contributing to normal pregnancy and impacting the outcomes of transplantation.
Before the therapeutic potential of cell replacement therapy or the development of therapeutic drugs for stimulating the body's own regenerative ability to repair cells damaged by disease and injury can be fully realized, control of stem cell fate, immuno-rejection, and limited cell sources must be overcome.
New techniques to study cell signaling and function can develop at a staggering pace; however, many approaches are as valid today as on the day they were established.
New high throughput techniques in neuroscience and psychiatry have enhanced the development of experimental, customizable animal models that are predictive of human neuropsychiatric pathology and give vital insights on the mechanisms and pathways involved.
This volume describes cell culture protocols for the maintenance, propagation, manipulation, and analysis of primary explanted cells from various mouse organ systems.
In 2010 the global area of transgenic crops reached 148 million hectares, an 87-fold increase since 1996, making it the most rapidly adopted technology in the history of modern agriculture.
This volume reviews available mouse models of atherosclerosis, basic aspects of disease initiation and progression, and the visualization of atherosclerotic lesions with invasive and non-invasive techniques that are widely used both in basic research and in the clinic.
Regulatory B Cells: Methods and Protocols present the current experimental set-ups and methodologies useful for the identification and characterization of B cells with suppressive functions and for the study of their biological and immunological properties.
This fully updated book brings together protocols to arm stem cell biologists with tools and approaches to continue uncovering the intricacies and regulatory mechanisms underlying stem cell biology.
This book comprises a collection of chapters describing topics from traditional radiation cytogenetic analysis methods to the modern fluorescence based-analysis and high throughput automatic analysis methods.
This book provides both historical perspective and practical information to support researchers either currently involved in genomic research on rats or planning to begin such a project.
As autism is associated with many rare diseases, this extensive bench book examines how no one single model can be used; multiple organism models are needed, each one corresponding to one of the diseases or to one aspect of a disease.
Our understanding of addiction and how it is treated has advanced remarkably over the past decades, and much of the progress is related directly to animal research.
Although embryonic stem cells currently enjoy the public limelight and show great pr- ise for cell based medical therapies, it is the adult stem cells which are responsible for the body's natural ability to fght disease, heal and recover, or fail and succumb to various maladies.
This volume provides protocols describing the isolation and culture of diverse cell types stemming from the skin and the use of these cells and cell constructs for wound healing, bioengineering applications, and translational medicine purposes.
Since the conception of this acclaimed series of volumes examining neural tissue culture, the expansion of neuroscience has continued to produce vital discoveries that utilize tissue culture methodologies.
