Monoclonal Antibodies: Methods and Protocols, Second Edition expands upon the previous edition with current, detailed modern approaches to isolate and characterize monoclonal antibodies against carefully selected epitopes.
This book delves into the complexities of melanoma, offering an in-depth examination of the disease alongside the latest advancements in diagnosis and treatment.
In Eosinophil: Methods and Protocols, experts in the field of eosinophil biology comprehensively provide detailed methodological insight into the study of this fascinating cell.
The action of antimicrobial peptides (AMPs), ranging from direct killing of invading pathogens to immune response modulation and other complex biological responses, has stimulated research and clinical interest for more than two decades, but the area is still burgeoning due to emerging discoveries in the functions, roles, and regulation of AMPs, thus making the study of antimicrobial peptides a multi-disciplinary and rapidly evolving field.
The HLA molecules are important regulators of the immune response through mediating antigen presentation and interaction between key immune mediating cells.
Immunology has made significant progress in the past decade, driven forward by rapidly advancing technology and a renewed interest in the vast realm of innate immunity.
It s not the dream that matters, it s the telling of the dream the words you choose, the risks you take in externalising your mindThis is a dreamlike portrait of a body in struggle to connect with itself and others.
This expert volume provides insights into the technological fundamentals together with a comprehensive overview of the potentialities of peptide microarray technology in basic research and clinical assays.
The first libraries of complementary DNA (cDNA) clones were con- structed in the mid-to-late 1970s using RNA-dependent DNA polymerase (reverse transcriptase) to convert poly A* mRNA into double-stranded cDNA suitable for insertion into prokaryotic vectors.
The chemokines family of small proteins are involved in numerous b- logical processes ranging from hematopoiesis, angiogenesis, and basal l- kocyte trafficking to the extravasation and tissue infiltration of leukocytes in response to inflammatory agents, tissue damage, and bacterial or viral infection.
Complement Systems: Methods and Protocols is composed of 32 individual chapters that describe a variety of protocols to purify and analyze the activity of the individual complement components or pathways.
The purpose of T Cell Protocols: Development and Activation is to c- lect a series of protocols, particularly those that have been developed within the past few years, to help investigators master new techniques (or improve existing ones) for the study of T-cell Biology.
Since the publication of the popular first edition, genomic methods have become more accessible, allowing antibiotic researchers to probe not only the sequence of antibiotic resistance determinants but the mechanism whereby they are expressed and regulated.
T-Helper Cells: Methods and Protocols presents a broad selection of cutting edge protocols that will enable the reader to capture the unique features of TH cells with tools developed for the isolation of TH cells from various tissues and subsequent analysis of their functional properties in vitro, ex vivo and in vivo.
The purpose of T Cell Protocols: Development and Activation is to c- lect a series of protocols, particularly those that have been developed within the past few years, to help investigators master new techniques (or improve existing ones) for the study of T-cell Biology.
As the research has continued, it has become increasingly clear that natural killer (NK) cells are critical sentinels of the innate immune response, playing important roles in protecting the body from numerous pathogens and cancer in addition to contributing to normal pregnancy and impacting the outcomes of transplantation.
For over forty years, mesenchymal stem cells (MSCs) have been scrutinized and studied, garnering much attention due to their broad therapeutic efficacy.
A continuously evolving technique, immunotherapy for the treatment of cancers now incorporates the use of immune cells infused during bone marrow transplants as well as approaches like cell and gene therapy, while stem cell-based therapies, tissue engineering, and targeting have also contributed to the latest successes in pre-clinical immunotherapy studies.
Unlike detecting constitutively expressed targets, immunohistochemical detection of labile, low abundance, and short-lived signal transduction molecules can be a very difficult task.
This book intends to introduce the basic concepts of B cell activation and its latest advances in biological research and disease treatment applications, including the structure and classification of B cell receptors, the process and mechanism of antibody-mediated B cell activation, and tumour immunity and monoclonal antibody therapies.
This book elucidates the diagnostic criteria and concept of asthma-COPD overlap (ACO) and provides the latest information on its epidemiology, pathophysiology, diagnostic criteria, and treatment options.
Clostridium difficile, a major nosocomial pathogen shown to be a primary cause of antibiotic-associated disease, has emerged as a highly transmissible and frequently antibiotic-resistant organism, causing a considerable burden on health care systems worldwide.
Immunoelectron microscopy is a key technique that bridges the information gap between biochemistry, molecular biology, and ultrastructural studies placing macromolecular functions within a cellular context.
Despite major advances in HIV treatment, many areas require more study, in order to create efficacious, potent antiretrovirals that can suppress viral load completely and durably without toxic side effects, to define unknown drug targets and fine-tune known targets, and to better understand the interplay between viral and host factors.
This volume combines protocols that encompass the true variety of investigation done on superantigens in the fields of microbiology, immunology, molecular biology, biochemistry, and cellular biology, with a strong focus on disease models utilized to determine the role of superantigens in human disease.
This volume details methods to detect Rhinovirus (RV) in clinical samples, propagation and purification of virus, in vivo and in vitro model systems, and the newer molecular tools that are useful for the studying the RV infection and pathogenesis.
Cytokines are pleiotropic regulatory proteins involved in essentially all biological processes and associated with a wide variety of diseases, including inflammatory disorders as well as many types of cancer and leukemia.
In light of the critical contributions of macrophages and dendritic cells to diverse inflammatory diseases and to immunity and host defense, state-of-the-art approaches to the investigation of their behavior are essential.
Immunotoxins represent a new class of human therapeutics that have widespread applications and a potential that has not yet been fully recognized since they were first conceived of by Paul Ehrlich in 1906.
The HLA molecules are important regulators of the immune response through mediating antigen presentation and interaction between key immune mediating cells.
In this first book dedicated entirely to the ELISPOT, a critical enzyme-linked immunospot assay used widely in biomedical research, recognized experts with first-hand experience detail how to design, perform, and analyze these assays.
This volume summarizes current cutting-edge methods related to carbohydrate-based vaccines, from the identification of a suitable carbohydrate antigen via the preparation of glycoconjugate vaccines to the characterization of vaccine candidates for their use in pre-clinical and clinical studies.
Volume Allergic Diseases is a comprehensive, multi-authored reference work, offering a broad appeal to microbiologists, immunologists, and infectious disease specialists.
This handbook, now in a new, second edition, is an essential resource for scientists with an interest in the role of glycosyltransferases and related genes involved in the biosynthesis of glycoproteins, glycolipids, and proteoglycans.
