Despite the availability of an effective vaccine, there are still 400 million people, worldwide who are chronically infected with hepatitis B virus (HBV).
There is now a range of cytotoxic drugs that have considerable clinical usefulness in producing responses in tumors and even, in a small proportion of cases, cure.
Enzyme-linked immunospot assay (ELISPOT) has been known for some time as a unique state-of-the-art technique for studying the cytokine-secreting activity of immune system cells, and it appears to be one of the fast growing applications in biomedical research, becoming an indispensable tool in vaccine development, HIV research, transplantation studies, and cancer and allergy research.
A collection of state-of-the-art molecular methods for studying antifungal resistance, for discovering and evaluating both new and existing antifungal drugs, and for understanding the host response and immunotherapy of such agents.
Leading basic researchers and clinical scientists describe in detail a wide variety of established and cutting-edge techniques they have developed to study the lifecycle and biological properties of the human papillomavirus.
If there is one aspect of current cancer research that represents a major ch- lenge in both novice and experienced researchers, it is the rapid advance in our understanding of the disease.
Since the first edition of Vaccinia Virus and Poxvirology: Methods and Protocols was published, a number of important events related to poxvirology have occurred, such as FDA approval of a culture-based live smallpox vaccine and the vaccination of large numbers of U.
Target discovery is a field that has existed for several years but is so vibrant today because of the recent progress in our understanding of the molecular mechanisms of many human diseases and the technical advances in target identification and validation.
From the early days when RNA interference was a strange artifact in worms to the 2006 Noble Prize received by Fire and Mello and the current clinical trials, the field of RNA interference has grown at a breakneck pace.
Avian Influenza Virus, Second Edition aims to provide the essential methods used in working with animal influenza viruses, and to compile more advanced information that will guide the user in designing influenza studies.
This volume describes the most common laboratory procedures for isolation, identification and characterization of polioviruses used in clinical and research laboratories.
Regulatory B Cells: Methods and Protocols present the current experimental set-ups and methodologies useful for the identification and characterization of B cells with suppressive functions and for the study of their biological and immunological properties.
Salmonella: Methods and Protocols presents detailed methods on a variety of aspects of Salmonella research, focusing on those which provide landmarks for future discovery.
Cells respond to environmental cues through a complex and dynamic network of signaling pathways that normally maintain a critical balance between cellular proliferation, differentiation, senescence, and death.
Continued refinement of wide-spread access to transgenic technology has allowed for new animal models have been developed that exhibit features of autoimmune disease have been developed that exhibit features of autoimmune disease.
Cancer Genomics and Proteomics: Methods and Protocols, Second Edition includes methods for the analyses of cancer genome and proteome that have illuminated us about the changes in cancer cells.
Meningococcal septicemia and meningitis continue to be important causes of devastating illness, death, and long-term disability in both developed and resource-poor countries of the world.
Lymphomas are lymphoid malignancies derived from B or T lymphocytes, and their study has been and still is paradigmatic for many aspects of cancer research.
Molecular Genetic Pathology, Second Edition presents up-to-date material containing fundamental information relevant to the clinical practice of molecular genetic pathology.
This collection seeks to elucidate the practical methods necessary for successful adjuvant development, with a particular focus on the synthesis, formulation, manufacturing, and characterization aspects involved.
The purpose of T Cell Protocols: Development and Activation is to c- lect a series of protocols, particularly those that have been developed within the past few years, to help investigators master new techniques (or improve existing ones) for the study of T-cell Biology.
Thisvolume summarizes current techniques; ranging from protocols for virus growth,isolation, quantification and generation of recombinant RSV virus to proceduresfor the efficient characterization of the host immune response to RSVinfection.
The discovery of Epstein-Barr virus (EBV) by Epstein, Achong, and Barr, reported in 1964 (Lancet 1:702-703), was stimulated by Denis Burkitt's rec- nition of a novel African childhood lymphoma and his postulation that an infectious agent was involved in the tumor's etiology (Nature194:232-234, 1962).
An authoritative collection of optimal techniques for producing and characterizing the immunologically active cells and effector molecules now gaining wide use in the clinical treatment of patients.
Enzyme-linked immunospot assay (ELISPOT) has been known for some time as a unique state-of-the-art technique for studying the cytokine-secreting activity of immune system cells, and it appears to be one of the fast growing applications in biomedical research, becoming an indispensable tool in vaccine development, HIV research, transplantation studies, and cancer and allergy research.
The third edition of thisvolume is aimed at providing both beginners and more experienced researchers achoice of methods to isolate and analyze dendritic cells(DC).
Unlike detecting constitutively expressed targets, immunohistochemical detection of labile, low abundance, and short-lived signal transduction molecules can be a very difficult task.
Given the vital importance of immune system research, the gathering of clear, consistent, and informative protocols involving the study of dendritic cells is paramount.
Vaccine research and development is advancing at an unprecedented pace, with an increasing emphasis on rational design based upon a fundamental und- standing of the underlying molecular mechanisms.
At the intersection of experimental and computational sciences, the second edition of Immunoinformatics provides biological insights as well as a simpler way to implement approaches and algorithms in the immunoinformatics research domain.
