Medical research

This volume not only discusses various common biobanking topics, it also delves into less-discussed subjects such as what is needed to start a biobank, training of new biobanking personnel, and ethnic representation in biospecimen research.

Thissecond edition expands upon the previous volume with new and updated chapters.

This book provides a practical guide to current methods for profiling and interpreting genomic alterations in tumors.

This volume provides methods to study bone morphogenetic proteins (BMPs) and BMP modulators in cell culture, in both invertebrate and vertebrate animal models, and for therapeutic applications.

This volume explores experimental approaches used to study Duchenne muscular dystrophy (DMD), an X-linked degenerative skeletal muscle disease caused by mutations in the dystrophin gene.

This volume provides key updates on several methods from the first edition as well as including new novel techniques to address the most recent technological developments and their applications in retinal research Chapters guide readers through gene identification approaches, detailed protocols to generate functional retinal pigment epithelium cells, mouse retina and other animal models, fundus imaging and angiography, and cell-based treatment approaches.

This volume provides a comprehensive collection of  classical and cutting edge protocols and techniques to examine the normal development and physiological functions of the gastrointestinal system and to model the most common digestive diseases.

This volume explores disease diagnosis and therapy in developing theranostics.

Infection by flaviviruses such as dengue virus serotypes (DENV 1-4), Japanese encephalitis virus (JEV), tick-borne encephalitis virus (TBE), yellow fever virus (YFV) and West Nile virus (WNV) impact millions of lives and cause tens of thousands of mortalities each year.

This detailed book explores methods to isolate, characterize, and investigate key lung innate immune cells.

Avian Influenza Virus, Second Edition aims to provide the essential methods used in working with animal influenza viruses, and to compile more advanced information that will guide the user in designing influenza studies.

This book explores the vital importance of T-cell differentiation in areas as wide-ranging as pathological analysis, drug development, and cell therapy of human T-cells.

This volume covers methods for determination of autoantibodies in rheumatic connective tissue diseases and organ-specific diseases.

This detailed volume introduces the reader to current methods in glaucoma research, in particular those that make use of animal disease models.

This book provides methodological information on establishing reliable and reproducible experimental models of cardiovascular diseases.

This collection thoroughly explores the dynamic and ever-developing field of hemostasis and thrombosis diagnostics and research.

The aim of this volume is to provide a wide range of techniques and aspects of mouse retinal phenotyping and characterization.

Somuchofwhatweknowaboutthepathogenesisofhumandiseasehascomefromthe systematic and careful study of histological material.

Presenting an area of research that intersects with and integrates diverse disciplines, including genomics, epigenetics, proteomics, and cellular biology, among others, Formalin-Fixed Paraffin-Embedded Tissues: Methods and Protocols collects contributions from expert researchers in order to provide practical guidelines to this complex study.

In recent years, allergy research has focused on the causes and mechanisms of allergy.

Ranging from the evolution of pathogenicity to oceanic carbon cycling, the many and varied roles that bacteriophages play in microbial ecology and evolution have inspired increased interest within the scientific community.

Diabetes is now reaching epidemic proportions, and the associated complications of this disease can be disabling and even life-threatening.

Continuous regeneration of the cornea is necessary to maintain this tissue in the transparent state that is essential for vision.

Over the past decades, knowledge about the cellular and molecular basis underlying the visual process has remarkably increased.

As the drug discovery process shifts more and more toward specifically targeting pathways and molecules, model systems continue to increase in importance, and the mouse, with its versatility, ease of use, and remarkable similarity to the human genome, has clearly risen to the forefront of animal model studies.

Clostridium difficile, a major nosocomial pathogen shown to be a primary cause of antibiotic-associated disease, has emerged as a highly transmissible and frequently antibiotic-resistant organism, causing a considerable burden on health care systems worldwide.

Tissue engineering and regenerative medicine represents a wide array of cell, biomaterial and cell/biomaterial based approaches focusing on the repair, augmentation, and regeneration of diseases tissues and organs.

As experimentation and clinical trials with first generation anti-angiogenic agents have yielded results and our understanding of the biology and physiology of blood and lymphatic vessels has increased, a new angiogenesis volume swiftly became a necessity.

Protein engineering is a fascinating mixture of molecular biology, protein structure analysis, computation, and biochemistry, with the goal of developing useful or valuable proteins.

Monoclonal Antibodies: Methods and Protocols, Second Edition expands upon the previous edition with current, detailed modern approaches to isolate and characterize monoclonal antibodies against carefully selected epitopes.

The pharmaceutical industry has faced many significant challenges since the early 1990s.

Since the conception of this acclaimed series of volumes examining neural tissue culture, the expansion of neuroscience has continued to produce vital discoveries that utilize tissue culture methodologies.

Drug delivery systems (DDS) are an important component of drug development and therapeutics.

Great disparities exist between organisms with regard to the relative ease of chromosomal mutagenesis and manipulation.

RNA interference (RNAi), in which RNA silences RNA, is the most recent discovery to revolutionize the study of biology.

Over the last decade the development of new molecular biology tools, advanced microscopy, live imaging and systems biology approaches have revolutionized our conception of how embryonic development proceeds.

In the last decade, a large number of major discoveries have shed light on the molecular mechanisms of lymphocyte migration and the anatomy of immune responses.

Microbiologists, medical mycologists, immunologists, and biochemists are increasingly working together to focus on the processes involved in the progression and treatment of fungal disease.

Hormone measurement is necessary for the diagnosis of a wide range of clinical conditions and is essential for monitoring the effectiveness of treatment.

Psychoneuroimmunology (PNI) has developed rapidly in the last four decades.

The sustained skin research efforts over the past decades has led to the accumulation of a significant collection of information on skin structure and physiology as well as on the pathogenesis of cutaneous diseases.

Despite the many milestones in cystic fibrosis (CF) research, progress toward curing the disease has been slow, and it is increasingly difficult to grasp and use the already wide and still growing range of diverse methods currently employed to study CF so as to understand it in its multidisciplinary nature.

In recent decades, infectious diseases, once believed to be fairly contained, have become a vital, resurgent field of research.

Androgens play a critical role in the development and maintenance of the male reproductive system and affect important physiological processes and pathological conditions, including the homeostasis of the normal prostate and prostate cancer.

Due to the rising threat of tuberculosis and other mycobacterial infections, methods to study the biology of the mycobacteria and to improve diagnostic, therapeutic and preventative reagents are still very much in need.

Our understanding of the apoptotic pathway and its regulation has improved significantly in the last 10 years.

The next revolution in molecular medicine is the application of molecular profiling to individualized patient therapy.

Recent critical advances in the field of immunotoxicology have led to incredible contributions to basic science, including crucial improvements in human risk assessment.

The liver is responsible for a wide range of critical functions essential to life, and is composed of several different cell types.