Medical research

As experimentation and clinical trials with first generation anti-angiogenic agents have yielded results and our understanding of the biology and physiology of blood and lymphatic vessels has increased, a new angiogenesis volume swiftly became a necessity.

Protein engineering is a fascinating mixture of molecular biology, protein structure analysis, computation, and biochemistry, with the goal of developing useful or valuable proteins.

Monoclonal Antibodies: Methods and Protocols, Second Edition expands upon the previous edition with current, detailed modern approaches to isolate and characterize monoclonal antibodies against carefully selected epitopes.

The pharmaceutical industry has faced many significant challenges since the early 1990s.

Since the conception of this acclaimed series of volumes examining neural tissue culture, the expansion of neuroscience has continued to produce vital discoveries that utilize tissue culture methodologies.

Drug delivery systems (DDS) are an important component of drug development and therapeutics.

Great disparities exist between organisms with regard to the relative ease of chromosomal mutagenesis and manipulation.

RNA interference (RNAi), in which RNA silences RNA, is the most recent discovery to revolutionize the study of biology.

Over the last decade the development of new molecular biology tools, advanced microscopy, live imaging and systems biology approaches have revolutionized our conception of how embryonic development proceeds.

In the last decade, a large number of major discoveries have shed light on the molecular mechanisms of lymphocyte migration and the anatomy of immune responses.

Microbiologists, medical mycologists, immunologists, and biochemists are increasingly working together to focus on the processes involved in the progression and treatment of fungal disease.

Hormone measurement is necessary for the diagnosis of a wide range of clinical conditions and is essential for monitoring the effectiveness of treatment.

Psychoneuroimmunology (PNI) has developed rapidly in the last four decades.

The sustained skin research efforts over the past decades has led to the accumulation of a significant collection of information on skin structure and physiology as well as on the pathogenesis of cutaneous diseases.

Despite the many milestones in cystic fibrosis (CF) research, progress toward curing the disease has been slow, and it is increasingly difficult to grasp and use the already wide and still growing range of diverse methods currently employed to study CF so as to understand it in its multidisciplinary nature.

In recent decades, infectious diseases, once believed to be fairly contained, have become a vital, resurgent field of research.

Androgens play a critical role in the development and maintenance of the male reproductive system and affect important physiological processes and pathological conditions, including the homeostasis of the normal prostate and prostate cancer.

Due to the rising threat of tuberculosis and other mycobacterial infections, methods to study the biology of the mycobacteria and to improve diagnostic, therapeutic and preventative reagents are still very much in need.

Our understanding of the apoptotic pathway and its regulation has improved significantly in the last 10 years.

The next revolution in molecular medicine is the application of molecular profiling to individualized patient therapy.

Recent critical advances in the field of immunotoxicology have led to incredible contributions to basic science, including crucial improvements in human risk assessment.

The liver is responsible for a wide range of critical functions essential to life, and is composed of several different cell types.

Presenting the most up-to-date techniques for the detection, genotyping, and investigation of methicillin-resistant S.

A versatile collection of readily reproducible cell-cell interaction assays for uncovering cellular interactions at the molecular level, both in vitro and in vivo.

According to the most recent clinical oncology data, one out of seven newly diagnosed malignancies worldwide result from infection and chronic inflammation in conjunction with cancer.

The aim of Single Cell Diagnostics: Methods and Protocols is for all readers to extend their knowledge and expertise in analysis of single cells.

Methods in Membrane Lipids presents a compendium of methodologies for the study of membrane lipids, varying from traditional lab bench experimentation to computer simulation and theoretical models.

Research in the pharmaceutical sciences and medicinal chemistry has taken an important new direction in the past two decades with a focus on large molecules, especially peptides and proteins, as well as DNA therapeutics.

Enzyme-linked immunospot assay (ELISPOT) has been known for some time as a unique state-of-the-art technique for studying the cytokine-secreting activity of immune system cells, and it appears to be one of the fast growing applications in biomedical research, becoming an indispensable tool in vaccine development, HIV research, transplantation studies, and cancer and allergy research.

Recently, important new findings in the polyamine field and a variety of new experimental systems have revolutionized the study of these ubiquitous cellular components, essential for normal growth and development.

Biological interactions of visible light with photosensitizers have been studied for over a century while controlled clinical applications of light and photosensitizers to treat solid tumors, known as photodynamic therapy, have been evolving since the mid 1970's.

Autophagy and phagocytosis are distinct yet partially morphologically similar processes.

Since the publication of the popular first edition, the explosion of DNA sequence information, the access to bioinformatics and mutation databases coupled with the ability to readily detect and confirm mutations has cemented the role of molecular diagnostics in medicine and, in particular, mutation detection by the polymerase chain reaction (PCR).

The first of two companion books addressed the biology and clinical aspects of ovarian cancer.

sCongenital heart disease is the leading cause of infant death and affects approximately one in every 100 babies born in the United States.

Many unexplored complex cellular and organismal adaptations occur in response to the stress of alcohol exposure, and its contribution to the development of chronic diseases, such as osteoporosis, heart disease and diabetes, is particularly relevant today, given the increased incidence of these diseases in our aging population.

The use of proteomics to study complex diseases such as cardiovascular disease, the leading cause of death in developed countries, has grown exponentially in recent years.

Microarray Technology, Volumes 1 and 2, present information in designing and fabricating arrays and binding studies with biological analytes while providing the reader with a broad description of microarray technology tools and their potential applications.

In recent years, the study of bone cells and tissues at the cellular and molecular levels in a variety of models has revolutionized the field.

As the expense of treating a growing number of end-stage kidney disease patients increases, greater attention has been paid to prevention and early treatment.

Immunologists today are interested in all of the diverse cell-types involved in host defense and have a deeper appreciation of the importance of innate immune mechanisms as a first line of protection against pathogens.

In Wound Regeneration and Repair: Methods and Protocols, expert researchers in the field detail classical and cutting-edge methods for studying wound healing and regeneration.

From the early days when RNA interference was a strange artifact in worms to the 2006 Noble Prize received by Fire and Mello and the current clinical trials, the field of RNA interference has grown at a breakneck pace.

During the past decade as the data on gene sequences and expression patterns rapidly accumulated, cell-free protein synthesis technology has also experienced a revolution, becoming a powerful tool for the preparation of proteins for their functional and structural analysis.

Increased knowledge on the pathogenesis of hematologic diseases has been translated into diagnostic and prognostic applications.

Monoclonal Antibodies: Methods and Protocols examines a collection of state-of-the-art methods that employ monoclonal antibodies in a clinical setting with opening chapters focusing on the gold standard method for generating mouse monoclonal antibodies through hybridoma technology, future methods for engineering recombinant and humanized antibodies, methods for engineering soluble Fc fusion protein, and the use of antibodies and flow cytometry in the quantification of cell signaling proteins.

Molecular biology has transformed prenatal diagnosis because it permits an accurate diagnosis to be made from very small quantities of fetal material, even single cells.

In recent years, there have been major advances in the concepts and methodologies used in the study of retinal development at both cellular and molecular levels.

Embryonic stem cells and adult stem cells are the two major types of stem cells that have been used for experimental and clinical studies.