Medical genetics

It is now 10 years since the first edition of YAC Protocols was published in 1996.

This volume details protocols that can be used for generation of knockout animals.

Microarray Technology, Volumes 1 and 2, present information in designing and fabricating arrays and binding studies with biological analytes while providing the reader with a broad description of microarray technology tools and their potential applications.

This volume focuses on RNAs interacting with chromatin and their function.

In Quantitative Trait Loci: Methods and Protocols, a panel of highly experienced statistical geneticists demonstrate in a step-by-step fashion how to successfully analyze quantitative trait data using a variety of methods and software for the detection and fine mapping of quantitative trait loci (QTL).

Transcription Factor Regulatory Methods details various techniques ranging from cutting-edge to general techniques use to study transcription factor regulatory networks.

RNA interference (RNAi), in which RNA silences RNA, is the most recent discovery to revolutionize the study of biology.

This volume provides clear and direct protocols to implement automated Design-Build-Test-Learn (DBTL) into synthetic biology research.

This volume provides a collection of protocols for the most common experimental methods used for engineering Yarrowia lipolytica.

This volume details the most important methods used for studying prokaryotic non-coding RNAs and their protein accomplices.

Plants are amazing organisms to study, some are important sources for pharmaceuticals, and others can help to elucidate molecular mechanisms required for a plant's development and its interactions with the biotic or abiotic environment.

Now in its second edition, this atlas serves as an easy-to-use diagnostic guide for the analysis of the human karyotype.

This volume aims at providing state-of-the-art protocols detailing ribosome display, cDNA display, phage display, yeast surface display, and mammalian display.

The past decade has seen the emergence of a new field of scientific inquiry at the intersection of phylogenetics and genomics: phylogenomics.

In the past decade, molecular biology has been transformed from the art of cloning a single gene to a statistical science measuring and calculating properties of entire genomes.

Over the past few years, DNA methylation technologies and our knowledge of DNA methylation patterns have been advancing at a breathtaking pace.

This volume provides experimental and bioinformatics approaches related to different aspects of gene expression analysis.

Animal Endo-SiRNAs: Methods and Protocols presents a variety of approaches to investigate endo-siRNAs.

In this volume expert researchers in the field detail many of the methods which are now commonly used to study RNA.

Innate DNA and RNA Recognition: Method and Protocols presents validated experimental strategies to dissect nucleic acid sensing in-vitro and in-vivo sources.

This completely revised and updated second edition to integrates the many new technologies and insights now available for the diagnosis of genetic diseases.

Despite the many milestones in cystic fibrosis (CF) research, progress towards curing the disease has been slow, and it is increasingly difficult to grasp and use the already wide and still growing range of diverse methods currently employed to study CF so as to understand it in its multidisciplinary nature.

This volume presents the latest protocols for both laboratory andbioinformatics based analyses in the field of marine genomics.

This volume provides readers with an introduction to all the methods and major approaches now being used to study the structural and functional development of the brain.

Chemogenomics aims toward the systematic identification of small molecules that interact with the products of the genome and modulate their biological function.

Synthetic mRNA is an attractive tool for mammalian cell reprogramming that can be used in basic research, as well as in clinical applications.

Great disparities exist between organisms with regard to the relative ease of chromosomal mutagenesis and manipulation.

In the early years of microarray technology, efforts were directed mainly at profiling expressed genes, while recently the microarray platform has been adapted into diverse applications directed toward the investigation of the physical genome.

This volume presents a comprehensive collection of detailed state-of-the-art protocols for gapmer-mediated RNA knockdown from leaders in the field.

Fluorescence in situ Hybridization (FISH) belongs to that special category of well-established molecular biology techniques that, since their inception a few decades ago, have succeeded in keeping a prominent position within the constantly expanding list of laboratory pro- dures for biomedical research and clinical diagnostics.

This volume focuses on cytological, biochemical, and molecular biological methods to identify and examine the function of each nuclear body, with an emphasis on the analysis of long non-coding RNAs.

The new techniques of molecular cytogenetics, mainly fluorescence in situ hybridization (FISH) of DNA probes to metaphase chromosomes or interphase nuclei, have been developed in the past two decades.

Together with early theoretical work in population genetics, the debate on sources of genetic makeup initiated by proponents of the neutral theory made a solid contribution to the spectacular growth in statistical methodologies for molecular evolution.

Due to their novel concepts and extraordinary high-throughput sequencing capacity, the "e;next generation sequencing"e; methods allow scientists to grasp system-wide landscapes of the complex molecular events taking place in various biological systems, including microorganisms and microbial communities.

In the last few years, significant advances have been made in the area of gene therapy for both genetic and acquired diseases.

This volume explores the applications of reporter gene technology and the methodologies needed for their effective implementation.

DNA microarray technology has revolutionized research in the past decade.

This volume provides readers with a wide collection of the latest and readily reproducible technical protocols available in the field ofnon-viral gene delivery vectors.

Capillary Electrophoresis: Methods and Protocols presents a selection of current capillary electrophoresis methods used to separate representative types of molecules and particles and in combination with different detection techniques.

Blood samples have consistently proven to be a key source of genetic material for a wide variety of diagnostic or research purposes.

Studies related to recombinant gene expression have brought new advance such as the emergence of the "e;omics"e; technologies.

This volume provides an overview of zygotic genome activation (ZGA) and the use of recent tools to elucidate the events that occur during ZGA.

The aim of this volume is to make computer programs for analyzing human genetic data more easily accessible to the beginner.

This volume provides a collection of methods and protocols detailing extracellular RNA and hopes to be a useful resource for those interested in this exciting and emerging field.

This volume explores methods and protocols for detecting epistasis from genetic data.

This volume introduces different concepts and methods of detecting RNA in biological material in a variety of model systems.

This third edition is comprised of well-established protocols that are considered the gold standard in the field as well as new methodologies for mitochondrial DNA analysis.