This book provides an understanding of the consequences of induced proteins in the toxicological response of cells to chemical and radiation damage to DNA and will be helpful in creating proper mathematical models for extrapolation to low doses and assessing human exposure or cellular injury.
This volume explores the latest methods used to study and define serpin molecular structure, basic protease inhibition, serpin targets, and the roles of serpin in biology and disease using animal models.
G Protein-Coupled Receptor Genetics: Research and Methods in the Post-Genomic Era features practical techniques inspired by the fast moving GPCR field.
The marvelous microbes that made life on Earth possible and support our very existenceFor almost four billion years, microbes had the primordial oceans all to themselves.
Small molecule microarrays (SMM) were introduced in 1999 and within just a short span of time, have established themselves as a vibrant next generation platform for high-throughput screening.
This volume presents a historical perspective on Morpholinos, an overview of good Morpholino practices, techniques for controlling Morpholino activity with light, techniques for modulating microRNA activity in zebrafish embryos, probing genes during fin regeneration, methods for determining to structure of gene networks during development, electroporation, bacterial knockdowns, pretargeting, diagnostic applications of Morpholinos, techniques for delivering Morpholinos in utero to developing embryos, and methods offering rapid, hands-free, label-free and inexpensive assays of Morpholino concentrations in biological extracts.
This book provides an up-to-date overview of key areas of ageing research and bridges the gap between the subcellular events and the reality of ageing as seen in clinical practice.
This detailed collection covers how the biological functions of histone deacetylases (HDACs) and histone acetyltransferases (HATs) can be detected in various experimental settings, both in vivo and in vitro.
In this highly anticipated update of the extremely successful Protein Targeting Protocols, experts from around the world provide the latest protocols on for isolating different organelles and the localization of particular proteins using a variety of methods such as light, confocal, and electron microscopy.
This volume explores various methodologies to study biochemical, molecular, and cellular biology aspects of some processes regulated by protein SUMOylation.
This detailed book expands upon the previous edition with a collection of methods for those performing experimental work on small GTPases of the Rho family.
Control of Cell Cycle and Cell Proliferation, Volume 135 in the Advances in Protein Chemistry and Structural Biology series, presents chapters on a variety topics, including Exploiting pivotal mechanisms behind the senescence-like cell cycle arrest, Viral infection on through Cell Cycle Regulation, Analyzing drug resistant mutation in CDK4 gene and identification of potential inhibitors through structure based virtual screening approach, Controlling cell proliferation by targeting CDK6 using drug repurposing approach, The role of the nucleolus in regulating cell cycle, Chromatin regulators in DNA replication and genome stability maintenance during S-phase, Role of macrophage in cancer cell progression and targeted immunotherapies, and much more.
This volume focuses on applications of split inteins, and the progress that has been made in the past 5 years on discovery and engineering of fast and more efficient split inteins.
Over the past thirty years, many elegant genetic and biochemical approaches have been combined in order to advance the study of protein secretion and the necessary navigation through cell membranes, yet, despite this progress, less than two hundred membrane protein structures are known, nowhere near the complete inventory that the discovered protein export systems suggest.
This detailed volume gathers basic and advanced methods and protocols from in vitro assays and in vivo models to address the molecular and functional aspects of tau physiopathology.
Cell-free protein expression promises to narrow the technological gap between DNA and protein technologies and provide a platform for broad application of synthetic biology principles in the Life Sciences.
Atomic Force Microscopy for Nanoscale Biophysics: From Single Molecules to Living Cells summarizes the applications of atomic force microscopy for the investigation of biomolecules and cells.
The Cardiovascular Glycocalyx in Health and Disease, Volume 91 gives updates on the glycocalyx function through the cardiovascular system and its integration in whole system physiology.
Originally published in 1990, this volume addresses issues surrounding global ecological changes and sustainability of present patterns of urbanisation and industrialisation.
Serial Analysis of Gene Expression (SAGE): Digital Gene Expression Profiling facilitates the introduction of SAGE into the laboratory, and provides a framework for interpreting and comparing data derived from SAGE experiments.
This volume introduces the reader to the field of enzyme stabilization and the different theories of enzyme stabilization, including the use of immobilization as a stabilization technique.
This volume aims to provide protocols on a wide range of biochemical methods, analytical approaches, and bioinformatics tools developed to analyze the proteome.
This detailed volume explores methods of examining gangliosides, membrane amphiphilic lipids that are deeply involved in the modulation of the overall properties of cellular membranes, as well as of the functions of membrane-associated proteins.
This volume focuses on explorative activity-based proteomics, biomedical applications of activity-based proteomics, and chemical strategies in activity-based proteomics providing a concise overview of activity-based protein profiling.
Unlike detecting constitutively expressed targets, immunohistochemical detection of labile, low abundance, and short-lived signal transduction molecules can be a very difficult task.
This volume provides a wide range of methods and protocols detailing various protein structures as platforms for building architectures with targeted application.
Hormones and Epigenetics, Volume 122, the latest release in the Vitamins and Hormones series, highlights new advances in the field, with this new volume presenting timely, interesting chapters written by an international board of authors.
Epigenetics in Health and Disease, Volume 197 in the Progress in Molecular Biology and Translational Science series, highlights new advances in the field, with this new volume presenting interesting chapters on An Introduction to epigenetics in health and disease, State-of-the-art techniques for epigenetics study, Computational biology in epigenetics, Artificial intelligence and machine learning in epigenetics, CRISPR-Cas systems in epigenetics modification for health, Mechanisms of DNA methylation and histone modifications, Non-coding RNA in epigenetics, Role of DNA Methylation in cardiovascular diseases, Role of epigenetics in renal diseases, and much more.
This detailed volume explores numerous histochemical techniques through a series of lab-tested protocols for the detection of specific molecules or metabolic processes, both at light and electron microscopy.
Featuring experimental approaches that shed light on the complexity of Ras GTPase biological functions, Ras Signaling: Methods and Protocols contains general overviews and detailed applications of both well-established and recently developed research techniques, including biochemical, biophysical, molecular biology, genetic and behavioral approaches, advanced high resolution fluorescence and electron microscopy imaging and "e;omics"e; technologies.
Volume 69 in the internationally acclaimed Advances in Clinical Chemistry contains chapters authored by world renowned clinical laboratory scientists, physicians and research scientists.
Addressing the numerous gaps in current information, Target Organ Toxicology in Marine and Freshwater Teleosts is an essential resource for researchers and professionals in aquatic toxicology and environmental risk assessment.
This book highlights the advances in essential oil research, from the plant physiology perspective to large-scale production, including bioanalytical methods and industrial applications.
