Cell membranes are not, as once believed, inert structures designed to contain the cell contents, but are in fact dynamic structures that are as me- bolically active as the cytosol and other cellular compartments they surround.
Interest in epitope mapping, or finding out where antibodies bind to their antigens, is by no means restricted to immunologists, but is shared by biolo- gists from a wide range of disciplines in which antibodies are used as molecu- lar reagents.
The continued successes of large- and small-scale genome sequencing projects are increasing the number of genomic targets available for drug d- covery at an exponential rate.
As the technology base for the preparation of increasingly c- plex peptides has improved, the methods for their purification and ana- sis have also been improved and supplemented.
Immobihzatron of enzymes, cells, and organelles has expanded greatly in the past 30 years as the advantages of immobilization have been evaluated and utilized in analyttcal, biotransformation, and medical applications.
The development of PCR, which enables extremely small amounts of DNA to be amplified, led to the rapid development of a multiplicity of a- lytical procedures that permit use of this new resource for the analysis of genetic variation and for the detection of disease-causing mutations.
The purpose of Ribozyme Protocols is to provide a helpful compilation of protocols that will be of use-^not only to those with some experience of ribozymes-^but also to those wishing to use ribozymes for the first time.
The synthesis of proteins from 20 or so constituent amino acids according to a strictly defined code with an accuracy of better than 1 in 10,000 at most loca- tions is arguably the most complex task performed by cells.
Since the discovery that protein kinase C (PKC) transduces the ab- dance of signals that result in phospholipid hydrolysis, this enzyme has been at the forefront of research in signal transduction.
As a scientist with an interest in proteins you will, at some time in your career, isolate an enzyme that turns out to be yellow-or perhaps you already have.
Lorette Javois' timely new 2nd edition revises and updates her widely acclaimed collection of step-by-step immunocytochemical methods, one that is now used in many biological and biomedical research programs.
The intent of this work is to bring together in a single volume the techniques that are most widely used in the study of protein stability and protein folding.
The development of PCR, which enables extremely small amounts of DNA to be amplified, led to the rapid development of a multiplicity of a- lytical procedures to utilize this new resource for analysis of genetic variation and for the detection of disease causing mutations.
Throughout the more than 20 years that have followed the beginnings of capillary electrophoresis (CE), its application to the analysis of proteins and peptides has continued to be reliable, versatile, and productive.
