Mammalian oocytes occupy a critical nexus in reproduction as they not only contribute half the genetic makeup of the embryo but also provide virtually all of the cytoplasmic building blocks required for sustaining embryogenesis.
Structural genomics is a newly emerging field that has arisen following the successful footsteps of the major sequencing efforts generally bundled under the heading "e;genomics"e;.
Although our understanding of the structure and activities of the cell nucleus and of the nanomachines which it contains is increasing rapidly, much remains to be learned.
Neuroproteomics: Methods and Protocols presents experimental details for applying proteomics to the study of the central nervous system (CNS) and its dysfunction through trauma and disease.
Since the publication of the first edition, the number of unique heritable mtDNA mutations recognized as being associated with bioenergetic dysfunction, cell death and disease has grown.
A landmark work covering the major aspects of the science, technology and application of membrane operations and related fields, from basic phenomena to the most advanced applications and future perspectives.
In addition to their invaluable role in the fight against infection, Toll-Like Receptors (TLRs) in an under- or over-active state can lead to the pathogenesis of disease, making these receptors a key focal point for many research laboratories.
Animal Cell Biotechnology: Methods and Protocols, Second Edition constitutes a comprehensive manual of state-of-the-art and new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture considering the whole cascade from lab to final production.
Once believed to be involved mainly with energetics, including the production of ATP, knowledge of the role of redox in the control of cellular activity has been expanded over recent years.
This volume captures the rapid developments in the field of induced pluripotent stem (iPS) cells, which have provided novel opportunities and approaches both for better understanding a number of human diseases and for developing new platforms for drug development and screening for such diseases.
Since the publication of the first edition, the number of unique heritable mtDNA mutations recognized as being associated with bioenergetic dysfunction, cell death and disease has grown.
Dictyostelium discoideum is a simple but fascinating eukaryotic microorg- ism, whose natural habitat is deciduous forest soil and decaying leaves, where the amoebae feed on bacteria and grow as independent single cells.
Now in two volumes, this completely updated and expanded edition of Embryonic Stem Cells: Methods and Protocols provides a diverse collection of readily reproducible cellular and molecular protocols for the manipulation of nonhuman embryonic stem cells.
As a high throughput method for analyzing gene function, cell-based microarrays have proven to be of vital importance, allowing high throughput analysis of over expression and knock down of proteins.
Interest in a specialized microenvironment or "e;niche"e; regulating hemopoietic stem cell function has been steadily growing since the idea was first proposed by Ray Schofield over three decades ago.
Human Fertility: Methods and Protocols is intended for all practitioners of reproductive medicine and ART, as well as for embryologists and reproductive, developmental, cell and molecular biologists and others in the biomedical sciences.
Hands-on researchers review the principles behind successful miniaturization and describe the key techniques for miniaturizing large-scale biochemical and bioanalytical methods for microchip analysis.
In the second edition of Cell-Cell Interactions: Methods and Protocols, expert researchers in the field detail a collection of protocols to examine interactions between cells.
In Apoptosis and Cancer: Methods and Protocols, Second Edition, expert researches in the field detail the performance of molecular and cellular biology techniques for studying and detecting the activation of the apoptotic pathway.
This book addresses the literature gap by providing both theoretical foundations and practical insights into in vitro plant secondary metabolite production.
Whether the question is one of basic cell survival, or whether it is being used to correlate cell number to some other factor such as matrix synthesis, an estimate of cell viability is universally required.
Neuroproteomics: Methods and Protocols presents experimental details for applying proteomics to the study of the central nervous system (CNS) and its dysfunction through trauma and disease.
Dictyostelium discoideum is a simple but fascinating eukaryotic microorg- ism, whose natural habitat is deciduous forest soil and decaying leaves, where the amoebae feed on bacteria and grow as independent single cells.
Animal Cell Biotechnology: Methods and Protocols, Third Edition constitutes a comprehensive manual of state-of-the-art and new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture from lab to final production.
Biomimetics and Stem Cells: Methods and Protocols collects a series of approaches to demonstrate the role and value of biomimetics for the better understanding of stem cell behavior and the acceleration of their application in regenerative medicine.
PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis.
The aim of Circadian Rhythms is to provide a resource that can be adopted by several types of users: those who are new to circadian biology, those who are already active in the field but are interested in learning new techniques and researchers who are considering moving to a new a model system or undertaking comparative studies and would like to consult protocols applied to different organisms before starting the study of new species.
This volume is the result of an explosion ofmolecular-based research on Cilia, which began with the discovery of theuniversality of intraflagellar transport (IFT) and ciliary genomics/proteomics.
This second edition expands on the first edition with new chapters describing methods for studyingcell movement, molecular components involved in chemotaxis, spatiotemporaldynamics of signaling components, and quantitative modeling, as well as severalupdated chapters from the first edition.
A highly anticipated update of the previous edition, In Vitro Transcription and Translation Protocols, Second Edition, provides molecular biology laboratories with the most powerful techniques for exploiting in vitro transcription and translation systems.
Reflecting over three decades of advances, Epidermal Cells: Methods and Protocols, Third Edition underscores these advances in our understanding of epidermal biology with updated and entirely new protocols that compliment and extend the earlier edition.
