Cellular biology (cytology)

Bacterial Artificial Chromosomes, Second Edition expands upon the previous edition with current, detailed methods developed for working with BACs.

This volume describes cell culture protocols for the maintenance, propagation, manipulation, and analysis of primary explanted cells from various mouse organ systems.

The volume provides comprehensive, state-of-the-art experimental techniques that are now available to dissect the molecular mechanisms of regulation and function of cohesin and the related factor condensin in vitro and in vivo across different model organisms, as well as in human cells.

This volume brings together the skills and protocols of numerous laboratories that are at the heart of investigation into the biology of Tfh cells in both mice and humans.

This volume details established experimental protocols on the isolation, characterization, and detection of extracellular matrix (ECM) molecules as well as methods to study the activity and role of ECM components on various biological functions, the formation of exosomes, and tissue surrogates.

This volume presents the latest collection of immunophenotypic techniques and applications used in research and clinical settings.

This volume provides leading-edge protocols in the study of the molecular and cellular biology of muscle stem cells.

Receptor Tyrosine Kinases: Methods and Protocols explores on the most widely studied of the protein classes, known for their central role in several cellular functions and in a variety of human pathologies.

The main aim of this fourth volume is to complement the first three volumes published in 2004 and 2012 by adding advanced methodologies and perspectives.

Focused on one of the most important and fascinating fields of modern biology, Exocytosis and Endocytosis, Second Edition presents a comprehensive collection of protocols for cutting edge, recently developed methods of studying exocytosis and endocytosis in vitro and in vivo.

This detailed book provides a wide range of techniques, from those that have been used extensively since the very first investigations into neural crest cells to those that are currently cutting-edge, in order to explore the development of neural crest cells in human, mice, rat, chick, quail, medaka, and shark.

Human Fertility: Methods and Protocols is intended for all practitioners of reproductive medicine and ART, as well as for embryologists and reproductive, developmental, cell and molecular biologists and others in the biomedical sciences.

This volume provides an up-to-date compilationof current methodological approaches utilized for the exploration of nucleolarstructure and function.

Tissue Morphogenesis: Methods and Protocols highlights major techniques, both experimental and computational, for the study of tissue morphogenesis, divided into several sections, with specific focus on techniques to image, manipulate, model and analyze tissue morphogenesis.

This updated volume explores a wide variety of clinical applications of PCR such as detecting DNA methylation, detection of viruses and protozoa in infectious diseases, estimation of gene copy number aberrations, primer extension coupled with mass spectroscopy, and high throughput NGS techniques.

This practical, hands-on volume examines the use of decellularized tissues and organs as biologically-active scaffolds by providing numerous approaches from experts in the field.

This volume provides a comprehensive look at the current fabrication methods, materials, cell patterning techniques, and practical application of live-cell microarrays.

This book comprises a collection of chapters describing topics from traditional radiation cytogenetic analysis methods to the modern fluorescence based-analysis and high throughput automatic analysis methods.

This volume features up-to-date protocols for the isolation, preservation, and validation of various cell sources comprising large and small animal models, examining the impact of cell transplantation on acute and chronic liver diseases.

Presentingstate-of-the-art protocols to study gap junctions, this detailed book firstfocuses on the use of methods and tools to investigate the different aspects ofconnexin expression and gap junction regulation.

Updated and revised, this thorough volume is organized such that it begins with techniques related to the study of chromatin structure.

This volume represents a valuable and readily reproducible collection of established and emerging techniques for neuronal cell death research.

Notch Signaling: Methods and Protocols focuses on molecular, biochemical and bioinformatics aspects of Notch signaling.

This second edition expands on the first edition with new chapters describing methods for studyingcell movement, molecular components involved in chemotaxis, spatiotemporaldynamics of signaling components, and quantitative modeling, as well as severalupdated chapters from the first edition.

This detailed volume provides thorough protocols describing how to use genetics to study mouse and zebrafish erythropoiesis in whole animal models and for genetically manipulating cultured mouse and human erythroid cells.

Regulatory B Cells: Methods and Protocols present the current experimental set-ups and methodologies useful for the identification and characterization of B cells with suppressive functions and for the study of their biological and immunological properties.

This detailed collection provides an accessible compendium of up-to-date methods focused on the study of RNAi and small regulatory miRNAs in stem cells.

This extensive volume explores areas of intense activity related to the very early commitment of stem cells to particular lineages and the progression of differentiation to mature cell stages.

This second edition provides updated and new chapters on selected genetic, molecular, biochemical, and cell biological techniques.

This volume captures the rapid developments in the field of induced pluripotent stem (iPS) cells, which have provided novel opportunities and approaches both for better understanding a number of human diseases and for developing new platforms for drug development and screening for such diseases.

This second edition volume expands on the previous edition with chapters discussing the latest developments and research initiatives in mitochondrial functions.

This volume discusses methods for the study of multipotent and pluripotentstem cells of the hair follicle.

This detailed book explores the most current techniques to study systems and epithelial cell culture.

This volume provides a comprehensive and technical presentation of numerous aspects of reproductive cell tissue cryopreservation, and presents readers with current procedures and detailed discussions of novel techniques and the latest innovations.

This thorough edition explores numerous methodological approaches that can be extremely informative for our understanding of the developmental toxicant mechanisms, characterization of developmental outcomes, and development of potential interventions that are clinically relevant.

This volume explores the latest techniques and approaches used to study this emerging form of cell death.

This book provides an overview of established 3D cell culture assays from leaders in the field.

This volume presents examples of how ANNs are applied in biological sciences and related areas.

This detailed volume presents a wide range of techniques that go beyond the standard assays typically used to assess Myogenesis.

Vascular Morphogenesis: Methods and Protocols provides a range of techniques for studying vascular morphogenesis in vivo and in vitro, reflecting advances in the field.

This volume discusses the requirements, advantages, and limitations of studying cell culture.

This volume details protocols on detection and analysis of fusion events in various cell types in vitro and in vivo.

This second edition explores membrane trafficking research that has been at the frontier of cell and molecular biology.

This volume presents an assortment of traditional and emerging experimental procedures relevant to Schwann cell research.

This detailed book collects new and updated techniques that will help to improve our understanding of the mechanisms underlying stem cell-derived repair through stem cells of the epidermal and dermal lineages, which have led to the isolation of numerous stem cell-like sub-populations from the epidermis and dermis.

This volume examines established methods and protocols to isolate and characterize extracellular vesicles (EVs) and their composition, among other techniques including purification, imaging, biofluid-specific and cell-specific isolation and downstream genomic and proteomic profiling.

This volume covers the latest technological advances in the characterization of the biosynthesis and functions of Rab GTPases and their regulation by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs).

This volume describes research methodologies and approaches used to study the Linker of Nucleoskeleton and Cytoskeleton (LINC) complex and its cellular functions.

This volume has been assembled for scientists interested in basic and applied biomedical research directed toward understanding the development, genetics and function of cardiomyocytes.

This volume provides a cross-section of RNA exosome research protocols, applied to a diversity of model organisms.