This volume describes the most common laboratory procedures for isolation, identification and characterization of polioviruses used in clinical and research laboratories.
Horizontal gene transfer (HGT) events encompass processes as varied as the exchange of genetic material between microbes coexisting in the same environment, between symbiotic bacteria and their eukaryotic hosts, and the evolution of organelles by symbiosis, in which whole genomes are acquired.
Adenovirus Methods and Protocols, Second Edition, now in two volumes, is an essential resource for adenovirus (Ad) researchers beginning in the field, and an inspirational starting point for researchers looking to branch into new areas of Ad study.
Due to the highly collaborative nature of investigators working in the field, we have rapidly advanced our understanding of Staphylococcus epidermidis and other staphylococci in the last two decades.
Given the popularity and utility of Saccharomyces cerevisiae, yeast-based functional genomics and proteomics technologies, developed over the past decade, have contributed greatly to our understanding of bacterial, yeast, fly, worm and human gene functions.
The huge potential for gene therapy to cure a wide range of diseases has led to high expectations and a great increase in research efforts in this area, particularly in the study of delivery via viral vectors, widely considered to be more efficient than DNA transfection.
Sexually transmitted infections (STI) continue to be a major cause of morbidity and mortality, both in developed industrial countries as well as in the developing world.
Sexually transmitted infections (STI) continue to be a major cause of morbidity and mortality, both in developed industrial countries as well as in the developing world.
Adenovirus Methods and Protocols, Second Edition, now in two volumes, is an essential resource for adenovirus (Ad) researchers beginning in the field, and an inspirational starting point for researchers looking to branch into new areas of Ad study.
This volume is designed to be a resource of proven techniques and approaches for probing the activities of bacterial, eukaryotic, and archaeal RNA polymerases.
Studies related to pathogen-mediated virus resistance in plants were instrumental in providing some of the historical observations which ultimately led to the vital discovery of double-stranded RNA (dsRNA)-induced gene silencing or RNA interference (RNAi), which has since revolutionized research on plant-virus interactions.
Although antiviral drugs have been successfully developed for some viral diseases, there remains a clear, unmet medical need to develop novel antiviral agents for the control and management of many viruses that currently have no or limited treatment options as well as a need to overcome the limitations associated with the existing antiviral drugs, such as adverse effects and emergence of drug-resistant mutations.
This Volume presents generic protocols for wet experimental and computer-based systems and synthetic biology approaches relevant to the field of hydrocarbon and lipid microbiology.
Homologous recombination is important in various aspects of DNA metabolism, including damage repair, replication, telomere maintenance, and meiosis, and yeast genetics has successfully provided a framework for the mechanism of homologous recombination.
Due to their novel concepts and extraordinary high-throughput sequencing capacity, the "e;next generation sequencing"e; methods allow scientists to grasp system-wide landscapes of the complex molecular events taking place in various biological systems, including microorganisms and microbial communities.
Protocols in Molecular Parasitology offers a wide range of experi- mental protocols, each written by experts, to research workers interested in exploring the molecular aspects of parasitology.
The closing years of the 19th century and the start of the 20th century witnessed the emergence of microbiology and immunology as discrete sci- tific disciplines, and in the work of Roux and Yersin, perhaps the first benefits of their synergy-immunotherapy against bacterial infection.
With the significant medical role that human cytomegalovirus plays in human disease (from acute disease following primary infection, to chronic disease due to life-long viral persistence), the need to better understand human cytomegalovirus biology and pathogenesis is needed.
Salmonella: Methods and Protocols, Second Edition expands upon the previous edition with current, detailed methods on different aspects and landmarks advancing knowledge on salmonella research.
Baculovirus Expression Protocols is a detailed guide for using the baculovirus expression vector system (BEVS) and/or insect cells to produce recombinant proteins.
Over the last decade, interest in mycoplasmas has been greatly sti- lated by the spread of contagious bovine pleuropneumonia (CBPP), caused by Mycoplasma mycoides subsp.
Following the considerable success of the first edition of Plant Virology Protocols, this exciting new edition covers the many new techniques that are now applied to the examination and understanding of plant viruses.
Host-Bacteria Interactions: Methods and Protocols details cutting edge protocols that cover aspects of the investigation of host bacteria interactions using mammalian and novel non mammalian infection models, cell biology, OMICS and bacterial genetics.
Filamentous fungi have long been known for their ability to produce an enormous range of unusual chemical compounds known as secondary metabolites, many of which have potentially useful antibiotic or pharmacological properties.
In addition to research and discovery, yeast surface display technology has found applications in industrial processes such as biofuel production and environmental pollutant absorption and degradation.
The methods included in Environmental Microbiology: Methods and Pro- cols can be placed in the categories "e;Communities and Biofilms,"e; "e;Fermented Milks,"e; "e;Recovery and Determination of Nucleic Acids,"e; and the review s- tion, containing chapters on the endophytic bacterium, Bacillus mojavensis, the engineering of bacteria to enhance their ability to carry out bioremediation of aromatic compounds, using the hemoglobin gene from a strain of Vitreoscilla 23 spp.
This volume bringstogether the most widely used and important protocols currently being employedin researching and understanding bacterial cell wall homeostasis.
Herpes Simplex Virus: Methods and Protocols provides a wide collection of protocols employed in various levels of herpes virus research, including basic protocols on growing viruses in cell culture and cloning, manipulating and preparing viral DNA.
Leading experts in enzyme manipulation describe in detail their cutting-edge techniques for the screening, evolution, production, immobilization, and application of enzymes.
The discovery of wide-spread RNA-based regulation in bacteria has led to new evaluations of the importance of bacterial regulatory RNA in every aspect of bacterial physiology.
Identifying Helicobacter infection as the leading cause of peptic ulcer disease and gastric cancer has dramatically altered the treatment of these disease states.
Systems Biology aims at deciphering the genotype-phenotype relationships at the levels of genes, transcripts (RNAs), peptides, proteins, metabolites, and environmental factors participating in complex cellular networks in order to reveal the mechanisms and principles governing the behavior of complex biological systems.
Due to the rising threat of tuberculosis and other mycobacterial infections, methods to study the biology of the mycobacteria and to improve diagnostic, therapeutic and preventative reagents are still very much in need.
