Immunology

This third edition volume expands upon the previous editions with new research topics and numerous leading-edge HIV protocols.

This volume presents a comprehensive collection of methods that have been instrumental to the current understanding of bacterial persisters.

In this volume, expert researchers detail protocols for evaluating the protean effects of immunosenescence on innate and adaptive immune function, including approaches to the analysis of antigen receptor repertoire.

This expert volume provides insights into the technological fundamentals together with a comprehensive overview of the potentialities of peptide microarray technology in basic research and clinical assays.

This volume combines protocols that encompass the true variety of investigation done on superantigens in the fields of microbiology, immunology, molecular biology, biochemistry, and cellular biology, with a strong focus on disease models utilized to determine the role of superantigens in human disease.

This volume presents standard approaches and the most recent technical advances used to study innate immune activation.

This volume summarizes current cutting-edge methods related to carbohydrate-based vaccines, from the identification of a suitable carbohydrate antigen via the preparation of glycoconjugate vaccines to the characterization of vaccine candidates for their use in pre-clinical and clinical studies.

This book provides researchers the opportunity to investigate type-2-associated diseases in their laboratories.

This second volume expands upon the previous edition with new research and objectives in immunoregulation and immune tolerance.

This third edition volume expands on the previous editions with more detailed research on the characterization of antibody antigen interactions between different users with different requirements.

This detailed volume utilizes our current understanding of the structural basis of multidrug recognition and multidrug efflux mechanisms to provide protocols involving these vital intrinsic membrane proteins widely distributed in bacteria.

This book details up-to-date and cutting edge compilation of protocols in mass cytometry.

This volume looks at the role of natural antibodies in pathogen elimination, cell survival, inflammation, cancer, and autoimmunity.

This volume looks at--and discusses the techniques of--a range of areas in biology in which macrophage migration inhibitory factor (MIF) is studied.

This volume provides comprehensive explanations and detailed examples of different antibody libraries, along with novel approaches for antibody discovery.

This detailed volume presents a set of protocols useful for researchers in the field of recombinant immunoglobulin and alternative scaffold engineering, aptamer development, and generation of molecularly imprinted polymers (MIPs).

This book details the most comprehensive, up-to -date, and cutting-edge protocols used in wet and dry labs to investigate the viral communities harbored within and on the human body.

This second edition provides 21 new chapters on methods used in laboratories for investigating the physiology and molecular genetics of the pathogen Clostridium difficile.

This volume provides a collection of methodologies for basic research, clinical diagnosis, and treatment pertaining to food allergens, including food allergen production, purification, characterization, detection, quantification, and bioinformatics approaches to modern food allergen studies.

This book explores well-established and emerging conjugation strategies that are relevant for proteins used in the field of precision medicine, focusing on techniques that are suitable for antibodies, antibody-fragments such as Fabs, scFvs, or nanobodies, scaffold proteins such as FN3 or DARPin, peptides, or model proteins.

This text provides a practical guide providing step-by-step protocol to design and develop vaccines.

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This book provides a selection of recently developed methods and protocols in bacterial glycomics to aid in bettering our understanding of the structures and functions of bacterial polysaccharides, their attachments to proteins and lipids, their role in biofilm formation, as well as their biosynthesis.

This volume covers a diverse collection of protocols currently being used by the Toxoplasma research community, and also looks at innovative methods that are pushing the boundaries of possibilities.

This detailed new edition provides complete and easy access to a variety of antibody engineering techniques.

This volume provides readers with methods and protocols for understanding the development of recombinant viruses and their use as vaccines platforms.

This detailed volume focuses on the development of mycosis vaccines via the most common etiological agents of mycoses, such as Aspergillus and Candida species, followed by Cryptococcus, dimorphic fungi, and members of Mucoraceae family of fungi.

This second edition provides updated and new chapters on T-Cell trafficking.

This second edition volume expands on the previous edition with a discussion of new and updated methods used to study the Herpes Simplex Virus (HSV), along with a look at the latest developing technologies such as next generation sequencing, CRISPR/Cas9 engineering, and the use of BioID to identify protein-protein interactions.

This volume explores the various methods used to study tertiary lymphoid structures (TLS) in pathological situations.

This collection seeks to elucidate the practical methods necessary for successful adjuvant development, with a particular focus on the synthesis, formulation, manufacturing, and characterization aspects involved.

This extensive volume covers basic and advanced aspects of peptide antibody production, characterization and uses.

In Basophils and Mast Cells: Methods and Protocols, experts in this challenging field explore techniques to research these cells from the most practical point of view.

This detailed volume describes a series of techniques that are essential for evaluating the efficacy of new checkpoint blockade therapies as well as understanding the mechanisms behind the therapies that have already been approved.

This volume explores the rapidly evolving field of HLA typing and its use in both the laboratory setting and in silico methods.

This volume provides key methods and protocols from laboratories engaged in germinal centers (GC) research with the expectation of stimulating further research, and to aid scientists in the study of GC biology and pathology.

This volume focuses on malaria vaccinology, and introduces researchers to a complete overview of the protocols and tools used by molecular and cellular malariologists.

This volume covers methods for determination of autoantibodies in rheumatic connective tissue diseases and organ-specific diseases.

This second edition volume expands on the previous edition with new sections describing the characterization of peptides bound to major histocompatibility complexes (MHC) on the surface of the cell.

This volume details the most updated concepts and experimental protocols developed by leading researchers in the field.

T-Helper Cells: Methods and Protocols presents a broad selection of cutting edge protocols that will enable the reader to capture the unique features of TH cells with tools developed for the isolation of TH cells from various tissues and subsequent analysis of their functional properties in vitro, ex vivo and in vivo.

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 This volume looks at key methodologies that are commonly used across antibody drug conjugates (ADCs) programs.

This second edition presents methods and protocols to aid readers in the design and execution of experiments used to define critical elements associated with innate immune system function.

The chemokines family of small proteins are involved in numerous b- logical processes ranging from hematopoiesis, angiogenesis, and basal l- kocyte trafficking to the extravasation and tissue infiltration of leukocytes in response to inflammatory agents, tissue damage, and bacterial or viral infection.

Antigen processing and presentation, as a field, explores a broad range of protein interactions and functions, both intracellular (in the cytoplasm and in the endoplasmic reticulum) and at the cell surface (between T cells and MHC molecules).

The worldwide impact of infection with human immunodeficiency virus type 1 (HIV- is reflected in the cumulative number ofHIV- 1 infections, which is now predicted to exceed 40 million by the year 2000---equivalent to the n- ber of humans who perished in World War II.

Interleukins are a family of proteins that regulate the maturation, diff- entiation, or activation of cells involved in immunity and inflammation, and belong to a broader family termed cytokines.

The principle that antibodies can be used as cytochemical agents provided they are tagged with suitable markers has been evident for over 50 years.