Cytokines are pleiotropic regulatory proteins involved in essentially all biological processes and associated with a wide variety of diseases, including inflammatory disorders as well as many types of cancer and leukemia.
This second edition of Eosinophils: Methods and Protocols updates several techniques from the first edition together with novel in vitro and in vivo-based methodologies.
After decades of research in clinical transplantation, new techniques have been developed that permit a further understanding of the immune mechanisms underlying immune recognition of allografts and a more accurate and thorough evaluation of compatibility between donors and recipients.
In this volume, expert researchers detail protocols for evaluating the protean effects of immunosenescence on innate and adaptive immune function, including approaches to the analysis of antigen receptor repertoire.
This volume explores immune cell receptors that are used in the detection of microbes, either by binding directly to non-self molecules or through indirectly sensing microbe-associated cellular disturbances.
In light of the critical contributions of macrophages and dendritic cells to diverse inflammatory diseases and to immunity and host defense, state-of-the-art approaches to the investigation of their behavior are essential.
The core of this three-volume book deals with damage-associated molecular patterns abbreviated "e;DAMPs"e;, which are unique molecules that save life and fight for survival of all organisms on this planet by triggering robust inflammatory/immune defense responses upon any injury, including those caused by pathogens such as viruses and bacteria.
The discovery of Epstein-Barr virus (EBV) by Epstein, Achong, and Barr, reported in 1964 (Lancet 1:702-703), was stimulated by Denis Burkitt's rec- nition of a novel African childhood lymphoma and his postulation that an infectious agent was involved in the tumor's etiology (Nature194:232-234, 1962).
This second edition presents methods and protocols to aid readers in the design and execution of experiments used to define critical elements associated with innate immune system function.
This timely book discusses antimicrobial drug resistance, specifically, the resistance against the beta-lactam class of antibiotics by Gram-Negative bacteria.
Vaccine research and development is advancing at an unprecedented pace, with an increasing emphasis on rational design based upon a fundamental und- standing of the underlying molecular mechanisms.
This book reviews the relationship between receptors, carbohydrate moieties, and pathogenic surfaces and lectins' pathophysiology of immune responses and examines the mechanisms of action of the molecules for the treatment potentials.
An authoritative collection of optimal techniques for producing and characterizing the immunologically active cells and effector molecules now gaining wide use in the clinical treatment of patients.
This book serves as a guide for identifying and applying commonly used cell-based translational assays as well as for assessing the therapeutic potential of new immuno-oncology therapeutics and advancing their mechanism of action.
Clostridium difficile, a major nosocomial pathogen shown to be a primary cause of antibiotic-associated disease, has emerged as a highly transmissible and frequently antibiotic-resistant organism, causing a considerable burden on health care systems worldwide.
Spanning from discoveries in fundamental immunology to industrial and commercial concerns, the study of vaccine adjuvants has developed into an exciting area of work with great, vital potential in innovating techniques in which adjuvants may steer the immune system towards the responses required by unmet vaccination needs.
Interleukins are a family of proteins that regulate the maturation, diff- entiation, or activation of cells involved in immunity and inflammation, and belong to a broader family termed cytokines.
Soon after the first description of monoclonal antibodies in 1976, there was enormous interest in the clinical application of antibodies, especially in the context of cancer.
The first libraries of complementary DNA (cDNA) clones were con- structed in the mid-to-late 1970s using RNA-dependent DNA polymerase (reverse transcriptase) to convert poly A* mRNA into double-stranded cDNA suitable for insertion into prokaryotic vectors.
The third edition of thisvolume is aimed at providing both beginners and more experienced researchers achoice of methods to isolate and analyze dendritic cells(DC).
Immunoelectron microscopy is a key technique that bridges the information gap between biochemistry, molecular biology, and ultrastructural studies placing macromolecular functions within a cellular context.
This volume summarizes current cutting-edge methods related to carbohydrate-based vaccines, from the identification of a suitable carbohydrate antigen via the preparation of glycoconjugate vaccines to the characterization of vaccine candidates for their use in pre-clinical and clinical studies.
Driven by methodological success in identifying reliable lineage markers, regulatory T cells have quickly been recognized as the most numerous subset of immune regulators in the body with critical functions in a wide array of immune responses.
The rapidly growing field of antibody research is the result of many advancing technologies allowing current developments to take advantage of molecular engineering to create tailor-made antibodies.
As the research has continued, it has become increasingly clear that natural killer (NK) cells are critical sentinels of the innate immune response, playing important roles in protecting the body from numerous pathogens and cancer in addition to contributing to normal pregnancy and impacting the outcomes of transplantation.
Immunology has made significant progress in the past decade, driven forward by rapidly advancing technology and a renewed interest in the vast realm of innate immunity.
This volume combines protocols that encompass the true variety of investigation done on superantigens in the fields of microbiology, immunology, molecular biology, biochemistry, and cellular biology, with a strong focus on disease models utilized to determine the role of superantigens in human disease.
Hundreds of eponyms are used within the field of immunology-Petri dish, Crohn's disease, Bence Jones protein, Kupffer cells, Freund's adjuvant, Ouchterlony immunodiffusion, to name just a few-but most of us don't know much about the individuals who gave their names to these terms.
