Diseases and disorders

This third edition volume expands upon the previous editions with new research topics and numerous leading-edge HIV protocols.

This volume presents a comprehensive collection of methods that have been instrumental to the current understanding of bacterial persisters.

Cancer Genomics and Proteomics: Methods and Protocols, Second Edition includes methods for the analyses of cancer genome and proteome that have illuminated us about the changes in cancer cells.

This volume provides an overview of molecular biology tools for pathogen detection and characterization.

In this volume, expert researchers detail protocols for evaluating the protean effects of immunosenescence on innate and adaptive immune function, including approaches to the analysis of antigen receptor repertoire.

This expert volume provides insights into the technological fundamentals together with a comprehensive overview of the potentialities of peptide microarray technology in basic research and clinical assays.

This text provides a practical guide providing step-by-step protocol to design and develop vaccines.

This volume combines protocols that encompass the true variety of investigation done on superantigens in the fields of microbiology, immunology, molecular biology, biochemistry, and cellular biology, with a strong focus on disease models utilized to determine the role of superantigens in human disease.

This volume details basic principles of experimental and computational methods for the study of microRNAs in cancer research and, therefore, provides a firm grounding for those who wish to develop further applications.

This volume explores the latest developments in the study of the mechanisms, diagnostics, screening methods, and therapeutics of colorectal cancer.

This detailed volume explores the methods used for most of the recent approaches to suicide gene therapy of cancer, which exploits promoters that are specific to cancer cells, thereby ensuring (or greatly increasing the likelihood) that the therapeutic gene is expressed only in cancer cells.

This second edition volume expands on the previous edition with descriptions of recent developments in the field.

This volume provides a thorough guide of the study of multiple myeloma (MM)-from sample acquisition to the performance of molecular tests, and also discusses difficulties that arise during quality control and interpretation of results.

This third edition volume expands on the previous editions with discussions on the latest developments in 2D- and 3D-organoid cultures that allow for the execution of precision medicine, and in vitro studies of tumor cell growth and invasion.

This third edition volume expands on the previous editions with an update on the latest techniques used for the detection, genotyping, and investigating pathogenesis of Staphylococcus aureus in vitro and in vivo.

Herpes Simplex Virus: Methods and Protocols provides a wide collection of protocols employed in various levels of herpes virus research, including basic protocols on growing viruses in cell culture and cloning, manipulating and preparing viral DNA.

This volume presents standard approaches and the most recent technical advances used to study innate immune activation.

This volume summarizes current cutting-edge methods related to carbohydrate-based vaccines, from the identification of a suitable carbohydrate antigen via the preparation of glycoconjugate vaccines to the characterization of vaccine candidates for their use in pre-clinical and clinical studies.

Rhinoviruses: Methods and Protocols highlights the numerous molecular, cellular and in vivo tools now available to conduct human rhinovirus (HRV) research in an effort to increase understanding of the clinical disease caused by HRVs as well as the functions of its individual proteins and its replication.

This volume describes up-to-date techniques used in understanding the molecular biology of acute myeloid leukemia (AML) and addressing advances in diagnosis, classification, prognostication, and therapeutic strategies to potentially impact overall patient survival.

This book provides researchers the opportunity to investigate type-2-associated diseases in their laboratories.

This second volume expands upon the previous edition with new research and objectives in immunoregulation and immune tolerance.

This volume explores the latest techniques used to study Leishmania and other trypanosomatidae.

This vital book collects key methods that have supported advancements in the field of ebolavirus molecular biology given the pressing need for the advancement of techniques for diagnostics, the development of vaccines and antivirals, and for furthering our understanding of ebolavirus biology.

This third edition volume expands on the previous editions with more detailed research on the characterization of antibody antigen interactions between different users with different requirements.

This book explores the methionine dependence of cancer and its effects on aging, a great story of science that is not widely known.

This volume presents methods and protocols on modern analysis of steroid receptor and prostate cancer function.

This book explores cutting-edge methods to work with the notoriously difficult, but highly prevalent, obligate intracellular pathogen, Chlamydia trachomatis.

This detailed volume utilizes our current understanding of the structural basis of multidrug recognition and multidrug efflux mechanisms to provide protocols involving these vital intrinsic membrane proteins widely distributed in bacteria.

This volume provides insight into recent developments on experimental and clinical strategies for cancer gene therapy.

This book details up-to-date and cutting edge compilation of protocols in mass cytometry.

This volume provides a thorough overview of the Wilms' Tumour Gene (WT1).

This detailed volume provides diagnosticians and researchers with practical methodologies and approaches to tackle animal coronaviruses.

Thisvolume covers multidisciplinary approaches on various aspects of ChikungunyaVirus (CHIKV) research that was brought together from leading laboratoriesacross the globe.

This volume looks at the role of natural antibodies in pathogen elimination, cell survival, inflammation, cancer, and autoimmunity.

This volume explores various approaches for enrichment, detection, isolation, and molecular profiling of circulating tumor cells (CTCs).

This book provides a toolkit of novel research approaches for investigators to study diabetic nephropathy, including critical experimental models from the fly to the fish, cells in culture, and in vivo mammalian approaches.

This volume looks at--and discusses the techniques of--a range of areas in biology in which macrophage migration inhibitory factor (MIF) is studied.

This volume provides comprehensive explanations and detailed examples of different antibody libraries, along with novel approaches for antibody discovery.

This detailed volume presents a set of protocols useful for researchers in the field of recombinant immunoglobulin and alternative scaffold engineering, aptamer development, and generation of molecularly imprinted polymers (MIPs).

This book details the most comprehensive, up-to -date, and cutting-edge protocols used in wet and dry labs to investigate the viral communities harbored within and on the human body.

This volume provides resources, ideas, and bench manuals for the study of breast cancer.

This detailed volume compiles the best methodologies and experimental techniques to profile and extract maximal data from brain tumor stem cells (BTSCs), the experimental paradigm for brain cancer research that offers insights into cancer stem cell populations that may drive not only tumor initiation but tumor recurrence and patient relapse.

This book aims to provide a guide for virologists, translational researchers, and clinicians in the field of cancer research by providing reference protocols and methodologies from vector development through clinical translation.

This book comprises protocols describing systems biology methodologies and computational tools, offering a variety of ways to analyze different types of high-throughput cancer data.

This volume describes the most common laboratory procedures for isolation, identification and characterization of polioviruses used in clinical and research laboratories.

This second edition provides 21 new chapters on methods used in laboratories for investigating the physiology and molecular genetics of the pathogen Clostridium difficile.

This volume provides a broad spectrum of research models, techniques, and protocols.

This volume provides a collection of methodologies for basic research, clinical diagnosis, and treatment pertaining to food allergens, including food allergen production, purification, characterization, detection, quantification, and bioinformatics approaches to modern food allergen studies.

This book explores well-established and emerging conjugation strategies that are relevant for proteins used in the field of precision medicine, focusing on techniques that are suitable for antibodies, antibody-fragments such as Fabs, scFvs, or nanobodies, scaffold proteins such as FN3 or DARPin, peptides, or model proteins.