Diseases and disorders

Meningococcal septicemia and meningitis continue to be important causes of devastating illness, death, and long-term disability in both developed and resource-poor countries of the world.

This book serves as a guide for identifying and applying commonly used cell-based translational assays as well as for assessing the therapeutic potential of new immuno-oncology therapeutics and advancing their mechanism of action.

This volume presents techniques needed for the study of long non-coding RNAs (lncRNAs) in cancer from their identification to functional characterization.

This book details up-to-date and cutting edge compilation of protocols in mass cytometry.

This third edition volume expands on the previous editions with discussions on the latest developments in 2D- and 3D-organoid cultures that allow for the execution of precision medicine, and in vitro studies of tumor cell growth and invasion.

Systemic Lupus Erythematosus: Methods and Protocols describe a number of genetic, biochemical and immunological techniques.

This manual treats both conceptual and practical aspects of gastrointestinal polyposis syndrome, which is characterized by dermatologic manifestations.

Cells respond to environmental cues through a complex and dynamic network of signaling pathways that normally maintain a critical balance between cellular proliferation, differentiation, senescence, and death.

This volume provides a thorough guide of the study of multiple myeloma (MM)-from sample acquisition to the performance of molecular tests, and also discusses difficulties that arise during quality control and interpretation of results.

Clostridium difficile, a major nosocomial pathogen shown to be a primary cause of antibiotic-associated disease, has emerged as a highly transmissible and frequently antibiotic-resistant organism, causing a considerable burden on health care systems worldwide.

Spanning from discoveries in fundamental immunology to industrial and commercial concerns, the study of vaccine adjuvants has developed into an exciting area of work with great, vital potential in innovating techniques in which adjuvants may steer the immune system towards the responses required by unmet vaccination needs.

This book provides insights into the care of cancer patients in the intensive care unit in a comprehensive manner.

The contents of Colorectal Cancer: Methods and Protocols aim to instruct investigators in all the key genetic, cellular, and molecular biological methods of analyzing colorectal tumors.

With many recent advances, cancer cell culture research is more important than ever before.

This book provides a philosophical account of the normative status of killing in Buddhism.

Rhinoviruses: Methods and Protocols highlights the numerous molecular, cellular and in vivo tools now available to conduct human rhinovirus (HRV) research in an effort to increase understanding of the clinical disease caused by HRVs as well as the functions of its individual proteins and its replication.

Interleukins are a family of proteins that regulate the maturation, diff- entiation, or activation of cells involved in immunity and inflammation, and belong to a broader family termed cytokines.

Since the first recognition of outbreaks of cerebrospinal or spotted fever at the end of the nineteenth and the beginning of the twentieth centuries, the menace of the meningococcus has been high on the list of public health prio- ties.

This volume explores the latest developments in the study of the mechanisms, diagnostics, screening methods, and therapeutics of colorectal cancer.

This review volume integrates the advances in cancer biology, molecular imaging techniques and imaging probes for visualization and quantitative measurement of anatomical, functional, and molecular profiles of cancer.

This volume presents state of the art of methods that can be useful for both basic and translational researchers to conduct chemoprevention preclinical studies.

Host-Bacteria Interactions: Methods and Protocols details cutting edge protocols that cover aspects of the investigation of host bacteria interactions using mammalian and novel non mammalian infection models, cell biology, OMICS and bacterial genetics.

John Sinclair and a panel of expert investigators present a comprehensive collection of cellular and molecular techniques for the analysis of cytomegalovirus (CMV) biology and its pathogenetic mechanisms.

This book provides an extensive overview of the role of imaging in the detection, diagnosis, management, and follow-up of tuberculosis.

Since the first report of an engineered oncolytic virus, there has been a continuing and steady increase of interest in the field, and while bench research remains vital for the translation of research in this field, its success depends on breakthroughs in clinical studies.

Soon after the first description of monoclonal antibodies in 1976, there was enormous interest in the clinical application of antibodies, especially in the context of cancer.

The first libraries of complementary DNA (cDNA) clones were con- structed in the mid-to-late 1970s using RNA-dependent DNA polymerase (reverse transcriptase) to convert poly A* mRNA into double-stranded cDNA suitable for insertion into prokaryotic vectors.

This volume discusses the latest developments in cellular, molecular, biochemical, and imaging assays to study the biology and functions of T-cells.

The third edition of thisvolume is aimed at providing both beginners and more experienced researchers achoice of methods to isolate and analyze dendritic cells(DC).

This book provides a comprehensive summary of the latest knowledge regarding functional foods and new therapeutic strategies for neurodegenerative disorders through explaining specific mechanisms for natural remedies and functional foods as well as alternative treatment and supplementary approaches for neurodegenerative diseases.

The process of metastasis formation is hugely complex, as described in the introductory chapter of this book, and this complexity has led us to compile two volumes of methods, from a vastly divergent background that attempts to encompass the whole spectrum of cancer biology.

This book introduces the basics of cellular and molecular biology on a basic level including detailed explanations and examples.

Biomarkers are molecular indicators of a biological status and, as biochemical species, can be interrogated to evaluate disease status and therapeutic interventions.

This book encompasses broad aspects of Cryptosporidium research with established methods that have been improved and expanded over the years as well as recent cutting-edge techniques.

This detailed volume explores numerous methods used in basic science laboratories to characterize cancer-related biomarkers, vital for better managing cancer burden, including cancer risk assessment, cancer diagnosis, determining cancer progression, and therapeutic response.

This second edition volume brings together the experiences of leading scientists in the discipline of cancer gene profiling.

Immunoelectron microscopy is a key technique that bridges the information gap between biochemistry, molecular biology, and ultrastructural studies placing macromolecular functions within a cellular context.

This volume describes a range of methods used in complement laboratories and how to interpret the data.

This volume summarizes current cutting-edge methods related to carbohydrate-based vaccines, from the identification of a suitable carbohydrate antigen via the preparation of glycoconjugate vaccines to the characterization of vaccine candidates for their use in pre-clinical and clinical studies.

A cutting-edge collection of readily reproducible techniques for the isolation, culture, and study of activation and signaling in human mast cells.

Bacterial infections affect world health today as a leading cause of morbidity and mortality.

This volume presents the latest collection of immunophenotypic techniques and applications used in research and clinical settings.

This volume looks at the newest methods, materials, equipment, and technologies developed to study the cell biology of the pneumococcus.

This second volume expands upon the previous edition with new research and objectives in immunoregulation and immune tolerance.

This edition provides a detailed, up-to-date overview of methods used in the field of immune tolerance.

A crisis is developing in the treatment of infectious diseases, with microorganisms frequently showing resistance to currently prescribed antibiotics.

Driven by methodological success in identifying reliable lineage markers, regulatory T cells have quickly been recognized as the most numerous subset of immune regulators in the body with critical functions in a wide array of immune responses.

The rapidly growing field of antibody research is the result of many advancing technologies allowing current developments to take advantage of molecular engineering to create tailor-made antibodies.

The Methods in Molecular Medicine series is intended as a resource for both novice and experienced investigators attempting to diversify their tech- logical base in research.

This volume provides methods to analyze the meningococcus and its interactions with biologically relevant host cells and sites, to interrogate the population structure and biology of the meningococcus that defines its capacity to cause disease, and to aid in vaccine development and surveillance.