Diseases and disorders

This volume explores the latest techniques used to study Leishmania and other trypanosomatidae.

Diverse molecular, cellular, and environmental events must all come together to allow the successful formation of secondary cancers, metastases.

Over the past two decades, spectacular advances have been made in our understanding of the molecular genetics of cancer, leading to the pursuit of identifying genes that, when mutated, result in an increased susceptibility to the disease.

There is now a range of cytotoxic drugs that have considerable clinical usefulness in producing responses in tumors and even, in a small proportion of cases, cure.

Proteases decisively contribute to cancer development and promotion by regulating the activities of growth factors/cytokines and signaling receptors, as well as the composition of the extracellular matrix, thereby suppressing cell death pathways and activating cell survival pathways.

Providing current diverse approaches and techniques used to study the immunoproteome, Immunoproteomics: Methods and Protocols collects chapters from key researchers that deliver information to be used in diagnostics, disease progression, and vaccine correlates of protection analysis, to name but a few.

This detailed volume presents a set of protocols useful for researchers in the field of recombinant immunoglobulin and alternative scaffold engineering, aptamer development, and generation of molecularly imprinted polymers (MIPs).

Ovarian cancer is the most lethal malignancy of the female reproductive system and is also the fifth leading cause of cancer death in women.

Despite various difficulties, the field of gene therapy, particularly with regard to cancer, has accumulated a tremendous amount of vital pre-clinical and clinical data.

Over the past several years, a high diversity of regulatory cells and suppressive molecules has taken centre stage in the field of immunoregulation.

This book comprises protocols describing systems biology methodologies and computational tools, offering a variety of ways to analyze different types of high-throughput cancer data.

Reports of influenza-like illnesses date back to the Middle Ages, and outbreaks of influenza likely afflicted humans long before that.

This book provides a selection of recently developed methods and protocols in bacterial glycomics to aid in bettering our understanding of the structures and functions of bacterial polysaccharides, their attachments to proteins and lipids, their role in biofilm formation, as well as their biosynthesis.

This vital collection provides a unique set of techniques to explore the clinical, pathological, and research aspects of the management of patients with esophageal adenocarcinoma.

This volume explores various approaches for enrichment, detection, isolation, and molecular profiling of circulating tumor cells (CTCs).

Many questions remain unresolved as researchers continue to characterize and define the nature of normal immune homeostasis and determine how these processes are dysregulated in immunodeficiency, as well as in autoimmune and lymphoproliferative disorders.

This book aims to provide a guide for virologists, translational researchers, and clinicians in the field of cancer research by providing reference protocols and methodologies from vector development through clinical translation.

This book details the most comprehensive, up-to -date, and cutting-edge protocols used in wet and dry labs to investigate the viral communities harbored within and on the human body.

This volume provides resources, ideas, and bench manuals for the study of breast cancer.

This detailed volume compiles the best methodologies and experimental techniques to profile and extract maximal data from brain tumor stem cells (BTSCs), the experimental paradigm for brain cancer research that offers insights into cancer stem cell populations that may drive not only tumor initiation but tumor recurrence and patient relapse.

Leading basic researchers and clinical scientists describe in detail a wide variety of established and cutting-edge techniques they have developed to study the lifecycle and biological properties of the human papillomavirus.

Recent outbreaks of swine influenza and avian influenza, along with the remaining and in some cases expanding threats from HIV, dengue virus, and the viruses causing hepatitis, have reinforced the need for rapid, accurate and cost-effective diagnosis of viral disease.

In recent decades, infectious diseases, once believed to be fairly contained, have become a vital, resurgent field of research.

This volume describes the most common laboratory procedures for isolation, identification and characterization of polioviruses used in clinical and research laboratories.

This volume provides a collection of methodologies for basic research, clinical diagnosis, and treatment pertaining to food allergens, including food allergen production, purification, characterization, detection, quantification, and bioinformatics approaches to modern food allergen studies.

This text provides a practical guide providing step-by-step protocol to design and develop vaccines.

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The purpose of T Cell Protocols: Development and Activation is to c- lect a series of protocols, particularly those that have been developed within the past few years, to help investigators master new techniques (or improve existing ones) for the study of T-cell Biology.

This volume covers a diverse collection of protocols currently being used by the Toxoplasma research community, and also looks at innovative methods that are pushing the boundaries of possibilities.

This volume aims to provide a range of methods and protocols for studying tumor angiogenesis in vitro and in vivo to reflect advances in the field.

The introduction of monoclonal antibodies revolutionized immunology.

This volume provides readers with methods and protocols for understanding the development of recombinant viruses and their use as vaccines platforms.

Psychoneuroimmunology (PNI) has developed rapidly in the last four decades.

Cancer Vaccines: Methods and Protocols explores the manipulation and modification of immune cells, the manipulation and modification of tumor cells as well as the manipulation of immune/tumor interactions and various delivery mechanisms, with the overall end goal of evoking a tumor-specific response and overcoming the immuno-evasive mechanisms employed by the tumor cells.

This book provides a practical guide to current methods for profiling and interpreting genomic alterations in tumors.

Our understanding of the apoptotic pathway and its regulation has improved significantly in the last 10 years.

This volume details common experimental approaches in studies designed to illuminate various processes used to study cancer metabolism.

Target discovery is a field that has existed for several years but is so vibrant today because of the recent progress in our understanding of the molecular mechanisms of many human diseases and the technical advances in target identification and validation.

Continued refinement of wide-spread access to transgenic technology has allowed for new animal models have been developed that exhibit features of autoimmune disease have been developed that exhibit features of autoimmune disease.

This second edition provides updated and new chapters on T-Cell trafficking.

Leading clinicians and scientists in solid organ transplantation review the current status of the field and describe cutting-edge techniques for detecting the immune response to the allografted organ.

Cancer Cell Signaling: Methods and Protocols, Second Edition brings together the most recent advances in cancer cell signaling knowledge along with recent progress in the development of novel strategies and experimental cell models to study human cancer.

This volume provides detailed descriptions of prevailing and novel techniques used by experts in the study of PTEN function in disease and biology.

This second edition volume expands on the previous edition with a discussion of new and updated methods used to study the Herpes Simplex Virus (HSV), along with a look at the latest developing technologies such as next generation sequencing, CRISPR/Cas9 engineering, and the use of BioID to identify protein-protein interactions.

This volume explores disease diagnosis and therapy in developing theranostics.

Infection by flaviviruses such as dengue virus serotypes (DENV 1-4), Japanese encephalitis virus (JEV), tick-borne encephalitis virus (TBE), yellow fever virus (YFV) and West Nile virus (WNV) impact millions of lives and cause tens of thousands of mortalities each year.

In Apoptosis and Cancer: Methods and Protocols, Second Edition, expert researches in the field detail the performance of molecular and cellular biology techniques for studying and detecting the activation of the apoptotic pathway.

This detailed volume gathers a comprehensive collection of methods, protocols, and procedures used for the identification, characterization, and selection of cancer stem cells.

More than ever, antibodies are being recognized as a major drug modality in a variety of diseases, including cancer, autoimmune diseases, infectious diseases, or even neurodegenerative disorders.

Despite major advances in HIV treatment, many areas require more study, in order to create efficacious, potent antiretrovirals that can suppress viral load completely and durably without toxic side effects, to define unknown drug targets and fine-tune known targets, and to better understand the interplay between viral and host factors.