Diseases and disorders

This volume provides an overview of the field and practical hints for vaccinologists in academia and industry.

At the intersection of experimental and computational sciences, the second edition of Immunoinformatics provides biological insights as well as a simpler way to implement approaches and algorithms in the immunoinformatics research domain.

This volume presents the latest collection of immunophenotypic techniques and applications used in research and clinical settings.

This volume covers the mechanisms of pRb inactivation detailing repressive mechanisms commonly associated to cancer, and representative of the experimentally relevant tests used in the establishment of cancer diagnosis and prognosis.

This second edition volume provides detailed protocols that address the challenges of signal-transduction IHC.

Systemic Lupus Erythematosus: Methods and Protocols describe a number of genetic, biochemical and immunological techniques.

Salmonella: Methods and Protocols, Second Edition expands upon the previous edition with current, detailed methods on different aspects and landmarks advancing knowledge on salmonella research.

This volume explores the still undiscovered secrets of tumor immunology and cancer immunology by discussing the methods and techniques that world-renowned experts in the field use in their laboratories.

This book provides researchers with widely used techniques for the study of virology, focusing on molecular biology and imaging to encourage mechanistic investigation of virus-host interactions.

This volume provides all the essential protocols that are currently used to study the immune synapse.

This volume details cutting-edge protocols on the characterization of the genome, epigenome, proteome, metabolome and single-cell transcriptome of tumors and tumor-derived cultures.

The second edition volume expands on the previous edition with new and updated chapters on the latest methods used for studying presentation of antigenic peptides produced in the standard processing pathways for MHC class I and II molecules.

This book aims to provide scientists with tools and well-researched protocols to enable their research and to facilitate further progress in this common leukemia.

This book covers core and emerging in vitro and in vivo protocols used to study how various components of the tumor microenvironment are established and subsequently interact with tumor cells to facilitate carcinogenesis.

DNA Vaccines: Methods and Protocols, Third Edition explores innovative approaches and technologies used to design, deliver, and enhance the efficacy of DNA vaccines.

Thisvolume summarizes current techniques; ranging from protocols for virus growth,isolation, quantification and generation of recombinant RSV virus to proceduresfor the efficient characterization of the host immune response to RSVinfection.

This volume is a valuable and timely resource for a broad audience with interests in basic and translational cancer biology, cancer drug development, as well as in the practice of personalized oncology.

This third edition volume expands on the previous editions with new protocols and up-to-date techniques to help researchers excel in this field.

While HIV-1 continues to be well-researched, this detailed volume draws attention to other members of the Retrovirus family, namely the Human T-lymphotropic Viruses (HTLVs), featuring the most updated technical information about HTLV determination and the methods to investigate their interaction with the host immune system and interfering pathogens.

This volume delivers a compendium of detailed protocols to the research community in order to aid in the investigation and discovery of Yersinia virulence mechanisms using important in vivo and in vitro infection models, which have led to major advances in the field and in our understanding of Yersinia pathogen-host interactions.

Host-Bacteria Interactions: Methods and Protocols details cutting edge protocols that cover aspects of the investigation of host bacteria interactions using mammalian and novel non mammalian infection models, cell biology, OMICS and bacterial genetics.

This volume contains collection of Natural Killer Cell methodologies relevant for both basic and translational research.

This second edition volume expands on the previous edition with descriptions of recent developments in the field.

This volume is a practical biochemical guide to the Enzyme-Linked Immunosorbent Assay (ELISA), used to detect a target substance in a liquid sample.

This edition provides a detailed, up-to-date overview of methods used in the field of immune tolerance.

This volume discusses certain epigenetic changes recognized in early carcinogenic lesions and different tumors, as well as factors that alter the epigenome and epigenetic profile such as diet, alcohol, immunity, circadian rhythm, and more.

Regulatory B Cells: Methods and Protocols present the current experimental set-ups and methodologies useful for the identification and characterization of B cells with suppressive functions and for the study of their biological and immunological properties.

This volume is a collection ofresearch methods, techniques, and approaches to investigate the molecularbiology of West Nile Virus (WNV).

In Eosinophil: Methods and Protocols, experts in the field of eosinophil biology comprehensively provide detailed methodological insight into the study of this fascinating cell.

This volume provides detailed molecular analysis of the associated pathways and cell types involved in Celiac Disease, and embraces new technologies such as next generation sequencing.

This volume details protocols that broadly cover many aspects of basic and translational research on Borrelia burgdorferi.

This detailed volume explores the most popular antigen production and delivery strategies that have been tested in veterinary species.

This third edition volume expands upon the previous editions with new research topics and numerous leading-edge HIV protocols.

This volume presents a comprehensive collection of methods that have been instrumental to the current understanding of bacterial persisters.

Cancer Genomics and Proteomics: Methods and Protocols, Second Edition includes methods for the analyses of cancer genome and proteome that have illuminated us about the changes in cancer cells.

This volume provides an overview of molecular biology tools for pathogen detection and characterization.

In this volume, expert researchers detail protocols for evaluating the protean effects of immunosenescence on innate and adaptive immune function, including approaches to the analysis of antigen receptor repertoire.

This expert volume provides insights into the technological fundamentals together with a comprehensive overview of the potentialities of peptide microarray technology in basic research and clinical assays.

This text provides a practical guide providing step-by-step protocol to design and develop vaccines.

This volume combines protocols that encompass the true variety of investigation done on superantigens in the fields of microbiology, immunology, molecular biology, biochemistry, and cellular biology, with a strong focus on disease models utilized to determine the role of superantigens in human disease.

This volume details basic principles of experimental and computational methods for the study of microRNAs in cancer research and, therefore, provides a firm grounding for those who wish to develop further applications.

This volume explores the latest developments in the study of the mechanisms, diagnostics, screening methods, and therapeutics of colorectal cancer.

This detailed volume explores the methods used for most of the recent approaches to suicide gene therapy of cancer, which exploits promoters that are specific to cancer cells, thereby ensuring (or greatly increasing the likelihood) that the therapeutic gene is expressed only in cancer cells.

This second edition volume expands on the previous edition with descriptions of recent developments in the field.

This volume provides a thorough guide of the study of multiple myeloma (MM)-from sample acquisition to the performance of molecular tests, and also discusses difficulties that arise during quality control and interpretation of results.

This third edition volume expands on the previous editions with discussions on the latest developments in 2D- and 3D-organoid cultures that allow for the execution of precision medicine, and in vitro studies of tumor cell growth and invasion.

This third edition volume expands on the previous editions with an update on the latest techniques used for the detection, genotyping, and investigating pathogenesis of Staphylococcus aureus in vitro and in vivo.

Herpes Simplex Virus: Methods and Protocols provides a wide collection of protocols employed in various levels of herpes virus research, including basic protocols on growing viruses in cell culture and cloning, manipulating and preparing viral DNA.

This volume presents standard approaches and the most recent technical advances used to study innate immune activation.

This volume summarizes current cutting-edge methods related to carbohydrate-based vaccines, from the identification of a suitable carbohydrate antigen via the preparation of glycoconjugate vaccines to the characterization of vaccine candidates for their use in pre-clinical and clinical studies.