This volume explores experimental approaches used to study Duchenne muscular dystrophy (DMD), an X-linked degenerative skeletal muscle disease caused by mutations in the dystrophin gene.
This volume provides methods to study bone morphogenetic proteins (BMPs) and BMP modulators in cell culture, in both invertebrate and vertebrate animal models, and for therapeutic applications.
Recent critical advances in the field of immunotoxicology have led to incredible contributions to basic science, including crucial improvements in human risk assessment.
Infection by flaviviruses such as dengue virus serotypes (DENV 1-4), Japanese encephalitis virus (JEV), tick-borne encephalitis virus (TBE), yellow fever virus (YFV) and West Nile virus (WNV) impact millions of lives and cause tens of thousands of mortalities each year.
The advancements of medical technology, improvements in medical care, and increased patients' life span make pain research and related drug development high priorities for both the research community and pharmaceutical companies.
Methods in Membrane Lipids presents a compendium of methodologies for the study of membrane lipids, varying from traditional lab bench experimentation to computer simulation and theoretical models.
According to the most recent clinical oncology data, one out of seven newly diagnosed malignancies worldwide result from infection and chronic inflammation in conjunction with cancer.
Over the past decades, the pathogenesis, diagnosis, treatment and prevention of cardiovascular diseases have been benefited significantly from intensive research activities.
Enzyme-linked immunospot assay (ELISPOT) has been known for some time as a unique state-of-the-art technique for studying the cytokine-secreting activity of immune system cells, and it appears to be one of the fast growing applications in biomedical research, becoming an indispensable tool in vaccine development, HIV research, transplantation studies, and cancer and allergy research.
Biological interactions of visible light with photosensitizers have been studied for over a century while controlled clinical applications of light and photosensitizers to treat solid tumors, known as photodynamic therapy, have been evolving since the mid 1970's.
The avian influenza (AI) virus, which is the type A influenza virus adapted to an avian host, has been an important pathogen for the poultry industry worldwide for many years.
Since the publication of the popular first edition, the explosion of DNA sequence information, the access to bioinformatics and mutation databases coupled with the ability to readily detect and confirm mutations has cemented the role of molecular diagnostics in medicine and, in particular, mutation detection by the polymerase chain reaction (PCR).
This book reviews the application of Nanobiotechnology in the development of Nanomedicine, while also discussing the latest trends and challenges in the clinical translation of Nanomedicine.
As the expense of treating a growing number of end-stage kidney disease patients increases, greater attention has been paid to prevention and early treatment.
This second edition expands upon the previous volume with additional emphasis on recent innovation in basic renal research and has a more holistic approach on associated disorders such as complications associated with peritoneal dialysis, ischemic acute kidney injury, the sympathetic nervous system, and vascular calcification.
Molecular biology has transformed prenatal diagnosis because it permits an accurate diagnosis to be made from very small quantities of fetal material, even single cells.
Reflecting the development of powerful new tools and high-throughput methods to analyze adenoviral particles and their interactions with host cells, the third edition of Adenovirus Methods and Protocols calls upon experts in the field to convey advances in molecular biology, genomics and proteomics, imaging, and bioinformatics.
Recent advances in molecular and cellular biology techniques have significantly improved our ability to detect, monitor, model and study the underlying molecular basis and pathogenesis of leukemia, yet we are still in an early discovery stage and much more work is needed in order to develop better strategies to diagnose, classify and treat this biologically and clinically diverse disease.
In recent years, molecular imaging techniques have grown to be invaluable tools for molecular biology research and, to a more modest extent, clinical medicine.
In past decades, the area of bioinformatics has proved to be both dynamic and vital, producing a wide spectrum of novel approaches and assuming an increasingly important role in modern bio-technological development.
In the last decade, a large number of major discoveries have shed light on the molecular mechanisms of lymphocyte migration and the anatomy of immune responses.
The sustained skin research efforts over the past decades has led to the accumulation of a significant collection of information on skin structure and physiology as well as on the pathogenesis of cutaneous diseases.
A versatile collection of readily reproducible cell-cell interaction assays for uncovering cellular interactions at the molecular level, both in vitro and in vivo.
Many unexplored complex cellular and organismal adaptations occur in response to the stress of alcohol exposure, and its contribution to the development of chronic diseases, such as osteoporosis, heart disease and diabetes, is particularly relevant today, given the increased incidence of these diseases in our aging population.
The use of proteomics to study complex diseases such as cardiovascular disease, the leading cause of death in developed countries, has grown exponentially in recent years.
This volume provides key updates on several methods from the first edition as well as including new novel techniques to address the most recent technological developments and their applications in retinal research Chapters guide readers through gene identification approaches, detailed protocols to generate functional retinal pigment epithelium cells, mouse retina and other animal models, fundus imaging and angiography, and cell-based treatment approaches.
Monoclonal Antibodies: Methods and Protocols examines a collection of state-of-the-art methods that employ monoclonal antibodies in a clinical setting with opening chapters focusing on the gold standard method for generating mouse monoclonal antibodies through hybridoma technology, future methods for engineering recombinant and humanized antibodies, methods for engineering soluble Fc fusion protein, and the use of antibodies and flow cytometry in the quantification of cell signaling proteins.
This book addresses the stabilization of vaccine powders by spray drying and provides an overview of the current state of the art on a laboratory and industrial scale.
This volume focuses on the computational modeling of cell signaling networks and the application of these models and model-based analysis to systems and personalized medicine.
This book covers classic epidemiological designs that use a reference/control group, including case-control, case-cohort, nested case-control and variations of these designs, such as stratified and two-stage designs.
This book covers classic epidemiological designs that use a reference/control group, including case-control, case-cohort, nested case-control and variations of these designs, such as stratified and two-stage designs.
This book highlights recent research investigating psychological and neural mechanisms contributing to dysfunctional cognition in people with schizophrenia.
This volume presents a broad collection of state-of-the-art methods to study the biology of thermogenic fat using in vitro cell culture and animal models.
This volume focuses on the computational modeling of cell signaling networks and the application of these models and model-based analysis to systems and personalized medicine.
