One of the major challenges currently facing the scientific community is to understand the function of the multitude of protein-coding genes that were revealed when the human genome was fully sequenced.
Over the past twenty years, the development of chromatin immunoprecipitation, or ChIP, assays has immensely enhanced the biological significance of the multifaceted DNA-binding proteins.
Cancer Genomics and Proteomics: Methods and Protocols, Second Edition includes methods for the analyses of cancer genome and proteome that have illuminated us about the changes in cancer cells.
MRSA Protocols for Methods in Molecular Biology provides a comprehensive collection of the most up-to-date techniques for the detection and investigation of MRSA.
Comparative Genomics, Volume 2, provides a collection of robust protocols for molecular biologists beginning to use comparative genomic analysis tools in a variety of areas.
Over the past two decades, spectacular advances have been made in our understanding of the molecular genetics of cancer, leading to the pursuit of identifying genes that, when mutated, result in an increased susceptibility to the disease.
In the past few years, the application of proteomics to examine the molecular mechanisms underlying (mal-)functioning of the nervous system and brain disorders has risen steeply, which in many cases has yielded novel insights.
Human strength testing has advanced as technology has evolved; from evaluating strength in a general and unspecialized way through to more scientific methods being applied to help performance and prevent injury.
In Polyadenylation: Methods and Protocols, expert researchers in the field detail many of the protocols which are now commonly used to study polyadenylation.
This volume provides a collection of cutting-edge strategies in siRNA delivery that were developed and refined over the years with tried-and-true methods.
Over the last two decades advances in genotyping technology, and the development of quantitative genetic analytical techniques have made it possible to dissect complex traits and link quantitative variation in traits to allelic variation on chromosomes or quantitative trait loci (QTLs).
Getting Pregnant in the 1980s: New Advances in Infertility Treatment and Sex Preselection provides a comprehensive guide to the rapidly evolving field of reproductive medicine during a transformative decade.
Immunoinformatics: Predicting Immunogenicity In Silico is a primer for researchers interested in this emerging and exciting technology and provides examples in the major areas within the field of immunoinformatics.
Hepatocytes account for approximately 80% of the liver mass and play a significant role in various aspects of liver physiopathology, exhibiting unrivaled complexity and diversity of functions.
The huge potential for gene therapy to cure a wide range of diseases has led to high expectations and a great increase in research efforts in this area, particularly in the study of delivery via viral vectors, widely considered to be more efficient than DNA transfection.
In Functional Analysis of DNA and Chromatin, expert researchers in the field provide an overview of standard and more recent methods for the functional analysis of the genetic material.
Genomic imprinting, the process by which the non-equivalence of the paternal and maternal genomes is established, has been fascinating us for over three decades and has provided many emerging scientists with the chance to hit their stride in a frontier posing many unexpected questions and even more surprising answers.
Since each human is genetically distinctive, responding differently to disease-causing factors as well as drugs, the field pharmacogenomics arose to develop personalized medicine, or medicine that deals with the complexity of the human body.
Arthritis Research: Methods and Protocols is a compendium of data pertinent to the methods and protocols that have contributed to recent advances in molecular medicine in general, but to the molecular basis of rheumatic disease in particular.
In 1996, we organized a workshop, inter alia, at the National Research Co- cil in Milan under the generous sponsorship of the European Science Foun- tion.
We are entering a particularly fruitful period in evolutionary genetics, as rapid technological progress transforms the investigation of genetic variation within and between species.
Providing a spectrum of models that is reflective of the various species that can be utilized in experimentation on disorders across a broad range of developmental disabilities, this volume collects expert contributions involved in investigation of the causes, outcomes, treatment, and prevention.
This volume expands on statistical analysis of genomic data by discussing cross-cutting groundwork material, public data repositories, common applications, and representative tools for operating on genomic data.
Once a tedious, highly skilled operation, reverse-transcription polymerase chain reaction (RT-PCR) has become a routine and invaluable technique used in most laboratories.
Directed Evolution Library Creation: Methods and Protocols, Second Edition presents user-friendly protocols for both proven strategies and cutting-edge approaches for the creation of mutant gene libraries for directed evolution.
Metagenomics has proven to be a powerful tool for exploring the ecology, metabolic profiling, and comparison of complex microbial communities as well as its important applications in the mining of metagenomes for genes encoding novel biocatalysts and drug molecules for bioindustries.
Central to the synthesis of proteins, the performance of catalysis, and many other physiological processes, the aberrant expression of which can be linked to human diseases including cancers, RNA has proven to be key target for therapeutics as well as a tool for therapy.
