Plants are amazing organisms to study, some are important sources for pharmaceuticals, and others can help to elucidate molecular mechanisms required for a plant's development and its interactions with the biotic or abiotic environment.
Immunologists today are interested in all of the diverse cell-types involved in host defense and have a deeper appreciation of the importance of innate immune mechanisms as a first line of protection against pathogens.
Together with early theoretical work in population genetics, the debate on sources of genetic makeup initiated by proponents of the neutral theory made a solid contribution to the spectacular growth in statistical methodologies for molecular evolution.
In Situ Hybridization Protocols, Fourth Edition contains 21 protocols that utilize the in situ hybridization technology to document or take advantage of the visualization of specific RNA molecules.
The goal of the characterization and discovery of G protein-coupled receptors, arguably the most important class of signaling molecules in humans and other vertebrates, has spawned numerous vital methodologies.
Continued refinement of wide-spread access to transgenic technology has allowed for new animal models have been developed that exhibit features of autoimmune disease have been developed that exhibit features of autoimmune disease.
A DNA barcode in its simplest definition is one or more short gene sequences taken from a standardized portion of the genome that is used to identify species through reference to DNA sequence libraries or databases.
Not only is the quantity of life science data expanding, but new types of biological data continue to be introduced as a result of technological development and a growing understanding of biological systems.
The study of functional glycomics requires the continuous development of rapid and sensitive methods for the identification of glycan structures and integration to structure-function relationships.
Recent work has revealed that stabilizing G-quadruplexes in telomeric DNA inhibits telomerase activity, providing impetus for the development of G-quartet-interacting drugs, while G-quartet-containing oligonucleotides have been recognized as a potent class of aptamers effective against STAT3 and other transcription factors implicated in oncogenesis, proving these guanine-quartets to be a vital and rich area for future study.
Arising from firm foundations laid by mathematical population genetics, clinical genetics, and statistical epidemiology, genetic epidemiology attempts to identify the many components of riskattributable to genes, environments, and interactions, and the course of its research can follow many diverse paths.
Research into ancient DNA began more than 25 years ago with the publication of short mitochondrial DNA sequence fragments from the quagga, an extinct relative of the zebra.
Activation, inhibition, or destruction of the nervous system or its component parts as a vital tool for the investigation of function has undergone remarkable development; indeed, new approaches have been developed that allow for these actions to be used as therapeutic tools.
The completion of a consensus draft sequence for the human genome was the starting point for more thorough investigations of individual genome variation.
Hepatocytes account for approximately 80% of the liver mass and play a significant role in various aspects of liver physiopathology, exhibiting unrivaled complexity and diversity of functions.
Capillary Electrophoresis: Methods and Protocols presents a selection of current capillary electrophoresis methods used to separate representative types of molecules and particles and in combination with different detection techniques.
The generation of genetically modified mice is absolutely crucial to gene function studies today, primarily because mice are genetically similar to man and because gene function studies in mice are in the context of a whole organism, making them particularly useful.
The new techniques of molecular cytogenetics, mainly fluorescence in situ hybridization (FISH) of DNA probes to metaphase chromosomes or interphase nuclei, have been developed in the past two decades.
The book is divided into four parts: the first part introduces the basic concepts of nicotinamide coenzymes, the synthesis of nicotinamide coenzymes, intracellular distribution, and an overview of the main functions and detection methods so that readers can quickly understand the general feature and function of nicotinamide coenzymes; the second part describes the involvement of nicotinamide coenzymes in intracellular signal transduction and their roles in cellular physiological and biochemical processes to explain exactly how nicotinamide coenzymes work in the processes and enable readers to deeply understand the principle of nicotinamide coenzymes playing an important function; the third part describes the role of nicotinamide coenzymes in the pathophysiological process of diseases, tries to illustrate the specific mechanisms of their association with the occurrence of the diseases, and points out the potential value in future translational medicine research; the fourth part introduces the production technology of nicotinamide coenzymes, including precursors, the process of industrial production, and the application of nicotinamide coenzymes and precursors in health industry, medicine, and life science research.
MRSA Protocols for Methods in Molecular Biology provides a comprehensive collection of the most up-to-date techniques for the detection and investigation of MRSA.
The primary purpose of this volume is to demonstrate the range of applications of the Pyrosequencing technology in research and diagnostics and to provide detailed protocols.
In miRNA Maturation: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study miRNA maturation.
Homologous recombination is important in various aspects of DNA metabolism, including damage repair, replication, telomere maintenance, and meiosis, and yeast genetics has successfully provided a framework for the mechanism of homologous recombination.
The tiny microRNAs (miRNAs) can have huge impacts on the regulation of a variety of genes and play crucial roles in the fundamental cellular processes.
MicroRNAs constitute a particularly important class of small RNAs given their abundance, broad phylogenetic conservation and strong regulatory effects, with plant miRNAs uniquely divulging their ancient evolutionary origins and their strong post-transcriptional regulatory effects.
Due to the vital biological importance of RNA and proteins functioning together within a cell, a protocol volume describing experimental procedures to study their interactions should find a home in many laboratories.
In the past decade, molecular biology has been transformed from the art of cloning a single gene to a statistical science measuring and calculating properties of entire genomes.
This manual offers detailed protocols for fluorescence in situ hybridization (FISH) and comparative genomic hybridization approaches, which have been successfully used to study various aspects of genomic behavior and alterations.
