Biochemistry

Internationally recognized biomedical scientists describe recent technological breakthroughs and demonstrate their use in successful experimental designs.

In this second edition of a widely used classic laboratory manual, leading experts utilize the tremendous progress and technological advances that have occurred to create a completely new collection of not only the major basic techniques, but also advanced protocols for yeast research and for using yeast as a host to study genes from other organisms.

As the expense of treating a growing number of end-stage kidney disease patients increases, greater attention has been paid to prevention and early treatment.

In Plant Metabolic Flux Analysis, expert researchers in the field provide detailed experimental procedures for each step of the flux quantification workflow.

Through all of the recent progress provided by high throughput DNA sequencing technologies, it has become clearer and clearer that the study of proteins and protein organelles will be the key to unlocking our ability to manipulate cells and intervene in human disease.

Although initially described as major regulators of cytoskeletal remodeling, Rho GTPases have been implicated in the establishment of polarity, endocytosis, vesicle trafficking, morphogenesis, cytokinesis, transcriptional activation, cell cycle progression, and apoptosis, to mention a few.

With the sequencing of the human genome complete, the field of proteomics, still in its early stages, has become an important and informative field of biomedical research.

Comparative Genomics, Volume 2, provides a collection of robust protocols for molecular biologists beginning to use comparative genomic analysis tools in a variety of areas.

Over the past thirty years, the development of the Western blot has revolutionized the fields of biomedical research and medical diagnostics.

Plant Proteomics: Methods and Protocols, Second Edition presents recent advances made in the field of proteomics and their application to plant biology and translational research.

Due to their importance to cell biology, DNA topology-altering enzymes, collectively DNA topoisomerases and gyrases, have been studied thoroughly from a biochemical perspective, yet the expansion of research at the molecular and cellular levels continues to show a great deal of promise.

A compendium of readily reproducible and novel methods to manipulate DNA viruses and characterize their varied biological properties.

The natural, biological, medical, and related sciences would not be what they are today without the microscope.

After the deciphering of the human genome and the genomes of many other organisms, the investigation of the function of gene products and their orchestral interplay is now one of the most important challenges in the life sciences.

In Fluorescent Protein-Based Biosensors: Methods and Protocols, experts in the field have assembled a series of protocols describing several methods in which fluorescent protein-based reporters can be used to gain unique insights into the regulation of cellular signal transduction.

Few problems in protein biochemistry have proven to be as challenging and recalcitrant as the molecular description of nitrogenase, the catalyst of one of the most remarkable chemical transformations in biological systems: the nucleotide-dependent reduction of atmospheric dinitrogen to bioavailable ammonia.

This book, split into two volumes, presents a broad coverage of the principles and recent developments of sample preparation and fractionation tools in Expression Proteomics in general and for two-dimensional electrophoresis (2-DE) in particular.

Amino Acid Analysis (AAA) is an integral part of analytical biochemistry.

In Flavins and Flavoproteins: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study flavins and flavoproteins.

Over the course of the past decade, there have been remarkable advances in the study of human pathogenic fungi.

Since the discovery of a collagen-degrading protease in the tadpole tail in 1962, matrix metalloproteinase research has led to the discovery of more than twenty distinct vertebrate MMPs, along with a variety of homologues from diverse organisms such as the sea urchin, plants, insects, and nematode worms.

Exciting work in the past decade has revealed commonalities and differences among individual cell fusion events.

This volume describes prominentmethodologies developed by laboratories that have been leading the field ofquantitative proteomics by mass spectrometry.

This detailed volume addresses recent developments in phosphoproteomic techniques with a particular focus on the plant system.

Caspases, Paracaspases, and Metacaspacses: Methods and Protocols is a collection of laboratory protocols covering current methods that are employed to measure and detect activities of these proteases in diverse biological systems, ranging from unicellular organisms to mammals.

Protein-protein interactions (PPIs) are strongly predictive of functional relationships among proteins in virtually all processes that take place in the living cell.

This volume provides an overview of experimental procedures and state-of art methods to investigate FOXOs.

As the use of high-throughput screening expands and creates more interest in the academic community, the need for detailed reference materials becomes ever more pressing.

The basic principle of electron crystallography is to calculate a 3D density map by combining the amplitudes obtained from electron diffraction patterns with the experimental phases calculated from images of two-dimensional crystals of membrane or soluble proteins.

In Post-Transcriptional Gene Regulation, renowned authors present current technical approaches to most aspects of post-transcriptional control and provide a useful and versatile laboratory bench resource.

Whether the pursuit is commercially motivated or purely academic, engineering a novel biological catalyst is an enticing challenge.

Chloroplasts are essential for the survival and flourishing of life on Earth.

The proteome consists of a complex mixture of proteins each of which need to be folded correctly in order to function for the health of the organism, and many of these proteins require molecular chaperones to reach the correct conformation and, in some cases, to remain in a folded form.

With the advent of proteomics came the development of technologies, primarily mass spectrometry, which allowed high-throughput identification of proteins in complex mixtures.

Quantum Dots captures many diverse applications enabling utility in biological detection.

Through many recent remarkable developments, perhaps the most significant advancements in the study of transcriptional regulation are the development of genome-wide approaches for measuring gene expression, exemplified by gene chips (chip), and chromatin immunoprecipitation assays (ChIP) for measuring in vivo protein-DNA interactions at any genomic loci.

Modification of target protein properties by reversible phosphorylation events has been found to be one of the most prominent cellular control processes in all organisms.

Major advances in molecular biology, alternative RNA splicing, protein processing, identification of gene control elements, transgenic animals and bioinformatics open up multiple research avenues and allow for better understanding of neuropeptide production and function.

Over the past few years, the power and potential of proteomics has become widely recognized.

Expert laboratorians noted for their optimized methods describe in step-by-step detail how to successfully carry out tissue-based laser microdissection in their own laboratory using the different systems available and to apply a wide range of sophisticated molecular technologies to the microdissected tissue samples.

Immunochemical techniques have been in use for many years with early examples of bacterial strain typing dating back to the 1940s.

Thousands of proteins have been identified to be acetylated.

Autophagy and phagocytosis are distinct yet partially morphologically similar processes.

Since the publication of the first edition, lentivirus vector-based technologies, through in vitro and in vivo gene transfer in eukaryotic animal cells, continue to offer the most promising opportunities for curing genetic disorders, as well as cancer and infectious diseases.

Pyrosequencing(R) Protocols presents detailed protocols for the multidisciplinary application of Pyrosequencing(R) technology, all written by world-renowned experts in the field.

This volume explores the considerable efforts that have been directed towards the development of G Protein-Coupled Receptors (GPCR) screening assays in order to disclose GPCR acting compounds, elucidate signaling mechanisms or evaluate compound's efficacy.

The use of proteomics to study complex diseases such as cardiovascular disease, the leading cause of death in developed countries, has grown exponentially in recent years.

This volume explores techniques used to detect lipids attached to proteins, to analyze the function of lipid modifications, and to characterize the enzymes that add and remove lipids from proteins.

Investigations into Calabar beans (the dried ripe seeds of Physostigma venenosum) and their alkaloidal components compose a classical scientific journey throughout some one-and-a-half centuries and not only represent a fascinating aspect of the history of medicine but which is, moreover, still ongoing at the forefront of chemical and medical discovery.