Biochemistry

In this volume, expert practitioners present a compilation of methods of functional data analysis (often referred to as "e;systems biology"e;) and its applications in drug discovery, medicine, and basic disease research.

This volume examines a wide array of vital technologies for advancing our understanding of the receptor-mediated actions of estrogen.

In this volume expert researchers detail in silico methods widely used to study peptides.

Due in part to the selective nature of telomerase inhibition as an anticancer approach, the field has expanded considerably in the past decade.

This volume explores three main aspects of nitric oxide (NO) research: NO treatment and detection, NO modifications, and NO detoxification.

After the identification of a potential protein drug, the next critical step is the production of sufficient authentic material for testing, characterization, and clinical trials, which, when successful, leads to the need for robust methodologies for large-scale production, purification, characterization, viral inactivation, and continued testing of the final protein product.

This second edition provides updated and new chapters that detail comprehensive overview of all the existing methods on analyzing protein arginylation.

Over the past decade, there have been a variety of fundamental insights into ribozymes which have changed the perspective on RNA catalysis.

Since the publication of the bestselling second edition of John Walker's widely acclaimed Protein Protocols Handbook, there have been continual methodological developments in the field of protein chemistry.

Epithelial mucins are large complex cell surface and secreted glycoproteins produced by mucosal epithelial cells.

Since the first edition, published in 2001, genomics research has taken great strides.

This volume of Methods in Molecular Biology focuses on techniques to determine the function of a gene.

A collection of cutting-edge techniques for studying ubiquitin-dependent protein degradation via the proteasome.

This third volume provides comprehensive protocols on pre-analytical, analytical, plasma, and serum proteomics.

Receptor Endocytosis and Signalling in Health and Disease, Part B, Volume 196 highlights the different aspects of receptor endocytosis and signaling, covering several receptors which are associated with different organs and that play a key role in normal functioning in the body, including somatostatin, Mas receptor, AMPA, Dopamine, 5-HT1-2, GABA, GPCR, nuclear receptor, Integrin, BCR, CRHR1, etc.

Microbial processes are involved in food, chemical, pharmaceutical, cosmetics, energy, and new-material industries.

Biochemical Pathways and Environmental Responses in Plants: Part C, Volume 683 in the Methods in Enzymology series, highlights advances in the field.

Water is well known to be an essential component of all biological systems.

Sulfurtransferases: Essential Enzymes for Life stands as the first comprehensive resource on this increasingly important class of enzymes.

Volume 24, entitled Targeted Metallo-Drugs: Design, Development, and Modes of Action, of the series Metal Ions in Life Sciences (MILS), fosters inter-disciplinary research in the vibrant field of Biological Inorganic Chemistry.

Bank filtration (BF) is a natural water treatment process which induces surface water to flow in response to a hydraulic gradient through soil/sediment and into a vertical or horizontal well.

Bank filtration (BF) is a natural water treatment process which induces surface water to flow in response to a hydraulic gradient through soil/sediment and into a vertical or horizontal well.

Volume 24, entitled Targeted Metallo-Drugs: Design, Development, and Modes of Action, of the series Metal Ions in Life Sciences (MILS), fosters inter-disciplinary research in the vibrant field of Biological Inorganic Chemistry.

With its gentle climate, rich soils and bountiful coastal waters, Kent is a land of plenty when it comes to food and drink.

Microbial processes are involved in food, chemical, pharmaceutical, cosmetics, energy, and new-material industries.

Biochemical Pathways and Environmental Responses in Plants: Part C, Volume 683 in the Methods in Enzymology series, highlights advances in the field.

Sulfurtransferases: Essential Enzymes for Life stands as the first comprehensive resource on this increasingly important class of enzymes.

This second edition volume expands all chapters of the previous edition, which have been enhanced to cover the most recent developments, the current state of method research, and applications.

This volume details the importance of multiple experimental techniques and computational methods needed to obtain the comprehensive picture of protein complex structure, dynamics and assembly afforded by the emerging field of integrative structural biology.

Integrated Methods in Protein Biochemistry: Part B, Volume 678 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of topics, including Precise modification of native proteins, purification, and analysis of bioconjugates, NanoBiT-based methods to monitor the activation and modulation of RTKs, The interplay of G-protein y subunit and PLC- enzyme in PIP2 hydrolysis and downstream signaling, Structure and function of bacterial secretion system, Tools and protocols for probing protein sumoylation, Spectroscopic analysis of cysteine dioxygenase: a mammalian thiol-dioxygenase, DeGlyPHER: MS-based analysis of viral spike N-glycoforms, and more.

Developments and Applications of Enzymes from Thermophilic Microorganisms extensively presents the industrial application of thermophilic/hyperthermophilic enzymes.

Contaminants of emerging concern in the marine environment: current challenges in marine pollution reviews the available data in relation to contaminants of emerging concern (CECs) in the marine environment: main sources, transport pathways, distribution in seawater and sediments, bioaccumulation, and biological effects.

This volume provides new technologies on NRPSs and related carrier protein dependent synthases, including polyketide synthases (PKS) and fatty acid synthases (FAS).

Epigenetics in Health and Disease, Volume 198 in the Progress in Molecular Biology and Translational Science series, highlights new advances in the field, with this new volume presenting interesting chapters on Computational biology in epigenetics, Artificial intelligence and machine learning in epigenetics, CRISPR-dCas9 Systems for Epigenetic Editing for Therapeutic Applications, Epigenetics in heredity disease, Epigenetics in cancer development, diagnosis and therapy, Microbiota and epigenetics: health impact, Histone deacetylase (HDACs) Inhibitors: Clinical applications, Early epigenetic markers for precision medicine, Epigenetics of neurological diseases, Epigenetic Regulons in Alzheimer's disease, and Epigenetics in epilepsy.

Modifications and Targeting of Protein Termini, Volume 684 in the Methods in Enzymology series serial highlights new advances in the field, with this new volume presenting interesting chapters pn a variety of timely topics, including Optimizing purification and activity assays of N-terminal methyltransferase complexes, In vitro reconstitution of hierarchical steps of Arg/N-degron pathway, Identification of N-degrons and N-recognins by using peptide-pull-downs combined with quantitative proteomics, A decoupled Virotrap approach to study the interactomes of N-terminal proteoforms, Monitoring ADO-dependent proteolysis in cells using fluorescent reporter proteins, Site-specific a-N-terminal methylation on peptides through chemical and enzymatic methods, and more.

This updated volume reflects new and evolved techniques to study detection, profiling, and manipulation of microRNAs (miRNAs) in plants and animals.

This volume is a comprehensive and up-to-date collection of strategies, reproducible methods, and protocols for the in-depth analysis of Proteoglycans (PGs) and their glycan part, the GAGs.

A readily reproducible collection of established and emerging techniques for studying the interaction between proteins and ligands, including biochemical/bulk techniques, structure analysis, spectroscopy, single-molecule studies, and theoretical/computational tools.

This volume provides new technologies on NRPSs and related carrier protein dependent synthases, including polyketide synthases (PKS) and fatty acid synthases (FAS).

Co-chaperones are important mediators of the outcome of chaperone assisted protein homeostasis, which is the dynamic integration of the processes of protein folding, degradation and translocation to ensure that cellular function is finely tuned in space and time.

Epigenetics in Health and Disease, Volume 198 in the Progress in Molecular Biology and Translational Science series, highlights new advances in the field, with this new volume presenting interesting chapters on Computational biology in epigenetics, Artificial intelligence and machine learning in epigenetics, CRISPR-dCas9 Systems for Epigenetic Editing for Therapeutic Applications, Epigenetics in heredity disease, Epigenetics in cancer development, diagnosis and therapy, Microbiota and epigenetics: health impact, Histone deacetylase (HDACs) Inhibitors: Clinical applications, Early epigenetic markers for precision medicine, Epigenetics of neurological diseases, Epigenetic Regulons in Alzheimer's disease, and Epigenetics in epilepsy.

Modifications and Targeting of Protein Termini, Volume 684 in the Methods in Enzymology series serial highlights new advances in the field, with this new volume presenting interesting chapters pn a variety of timely topics, including Optimizing purification and activity assays of N-terminal methyltransferase complexes, In vitro reconstitution of hierarchical steps of Arg/N-degron pathway, Identification of N-degrons and N-recognins by using peptide-pull-downs combined with quantitative proteomics, A decoupled Virotrap approach to study the interactomes of N-terminal proteoforms, Monitoring ADO-dependent proteolysis in cells using fluorescent reporter proteins, Site-specific a-N-terminal methylation on peptides through chemical and enzymatic methods, and more.

This detailed volume explores poly(ADP-ribose) polymerases (PARPs) in the biology of eukaryotes and their relevance to human health.

This edited book provides the first comprehensive overview on conventional and emerging processing technologies for the extraction and purification of proteins and/or peptides from plant sources with a special focus on subsequent product development.

This book provides a state-of-the-art overview of key areas of subcellular aging research in human cells.

Scattering Methods in Structural Biology, Part B, Volume 676 in the Methods in Enzymology serial, highlights advances in the field, presenting chapters on Quality controls, Refining biomolecular structures and ensembles by SAXS-driven molecular dynamics simulations, Data analysis and modelling of small-angle scattering data with contrast variation, Observing protein degradation in solution by the PAN-20S proteasome complex: state-of-the-art and future perspectives of TR-SANS as a complementary tool to NMR, crystallography and Cryo-EM, Extracting structural insights from chemically-specific soft X-ray scattering, Reconstruction of 3D density of biological macromolecules from solution scattering, ATSAS- present state and new developments in computational methods, and much more.

Advances in Clinical Chemistry, Volume 71, is the latest installment in this internationally acclaimed series.

Written by authors representing the diverse field of clinical chemistry and diagnostics, reviews in Advances in Clinical Chemistry cover a range of cutting-eduge research ranging from basic biochemical exploration to microarray technology.

Advances in Clinical Chemistry, Volume 107, the latest installment in this internationally acclaimed series, contains chapters authored by world-renowned clinical laboratory scientists, physicians and research scientists.

Advances in Clinical Chemistry, Volume 78, the latest installment in this internationally acclaimed series, contains chapters authored by world-renowned clinical laboratory scientists, physicians, and research scientists.