Biochemistry

All three peroxisome proliferator-activated receptor (PPAR) subtypes share a high degree of structural homology but differ in function, tissue distribution and ligand specificity.

This book explores well-established and emerging conjugation strategies that are relevant for proteins used in the field of precision medicine, focusing on techniques that are suitable for antibodies, antibody-fragments such as Fabs, scFvs, or nanobodies, scaffold proteins such as FN3 or DARPin, peptides, or model proteins.

Exemplifying and illustrating recent exciting advances in PNA chemistry, the second edition of Peptide Nucleic Acids: Methods and Protocols serves as a vital complement to the first edition of the book.

Environmental genomics seeks to predict how an organism or organisms will respond, at the genetic level, to changes in their external environment.

Exploring the 2-D gel mapping field, the chapters in this book are separated into four different categories: Part I talks about 2-D maps reproducibility and maps modeling; Part II describes the image analysis tools that provide spot volume datasets; Part III is about the statistical methods applied to spot volume datasets to identify candidate biomarkers; and Part IV discusses differential analysis from direct image analysis tools.

This detailed new edition delves into recently developed protocols for those working on cell nucleus research, including some of the less well-explored areas of study.

It is now well established that jasmonates, originally identified as the major component of jasmine scent, play a universal role in the plant kingdom and are involved in the regulation of diverse aspects of plant biology, including growth, development, metabolism, and interaction with the environment.

This book presents a compilation of methods that detail improved protein and peptide sample preparation and identification.

Knowledge of the three-dimensional structure of a protein is absolutely required for the complete understanding of its function.

Amino Acid Analysis (AAA) is an integral part of analytical biochemistry.

Senior scientists Marilena Aquino de Muro and Ralph Rapley have brought together an outstanding collection of time-tested protocols for designing and using genes probes in a wide variety of applications.

The hematopoietic stem cell (HSC) field has rapidly grown in the past several years as new technologies have been developed and the older tried and true methods have been used in new ways.

Protocols books specializing in measuring free radical and antioxidant biomarkers began to be published in 1998.

The number of protein sequences grows each year, yet the number of structures deposited in the Protein Data Bank remains relatively small.

This volume focuses on screening and profiling kinase inhibitors or activators.

In the past several years significant attention has been given to the analysis of the properties and functions of lateral microdomains (rafts) in biological membranes.

The transfer of hereditary information from genes to proteins is one of the essential pr- esses in all living organisms on our planet.

SELDI is distinct from other TOF-MS technologies in that it couples features of chromatography and mass spectrometry, facilitating analyte enrichment and sample cleanup on an array surface.

In this volume expert researchers in the field detail the operations of microchip capillary electrophoresis.

This volume explores the latest synthetic procedures for producing receptor-specific retinoids and rexinoids, molecular biology methods, and new technologies to demonstrate the therapeutic activities of molecules.

The orchid family is one of the largest families of flowering plants known for their beauty and economic importance.

Advanced Protocols in Oxidative Stress III continues the thread of the first two books by covering technology ranging from a portable hand-held detector for remote analysis of antioxidant capacity to sophisticated technology such as shotgun lipidomics, mitochondrial imaging, nano sensors, fluorescent probes, chromatographic fingerprints, computational models and bio statistical applications.

In recent years there has been a tremendous increase in our understanding of the functioning of the cell at the molecular level.

This thorough book aims to present the methods that have enabled the success of peptides and proteins in a wide variety of applications.

The introduction of high-performance liquid chromatography (HPLC) to the analysis of peptides and proteins some 25 years ago revolutionized the biological sciences by enabling the rapid and sensitive analysis of peptide and protein structure through the exquisite speed, sensitivity, and resolution that can be easily obtained.

Over the last ten years, Green Fluorescent Proteins, along with the other spectral variants, have emerged from near obscurity to become a powerful and versatile tool in scientific research.

While many cytokines are known for their inflammatory action, there is a growing interest in the tissue-protective effects of some cytokines.

This detailed volume describes cutting-edge techniques in three distinct and complementary areas of contemporary kinase biology research.

Proteomics is a rapidly expanding investigation platform in cardiovascular medicine.

There is a demand for analytical methods that are able to discriminate between enantiomers in order to analyze the enantiomeric purity of compounds from natural or chemical sources not only in pharmaceutical sciences but in any field on bioactive compounds including chemistry, biology, biochemistry, forensic, and environmental sciences and many others.

A collection of standard and cutting-edge techniques for using Xenopus oocytes and oocytes/egg extracts to reconstitute biological and cellular processes.

It is increasingly clear that signal transduction is a highly organized and integrated process.

Peptide Characterization and Application Protocols is dedicated entirely to the characterization of peptides and their applications for the study of biochemical systems and the contributing authors are all leaders in the field of peptide research.

Plant Isoprenoids: Methods and Protocols is a collection of detailed techniques that will be a useful tool for a wide range of plant biologists, as well as for scientists of other fields interested in plant isoprenoids.

This book compiles key protocols instrumental to the study of high-throughput protein production and purification which have been refined and simplified over the years and are now ready to be transferred to any laboratory.

MicroRNAs constitute a particularly important class of small RNAs given their abundance, broad phylogenetic conservation and strong regulatory effects, with plant miRNAs uniquely divulging their ancient evolutionary origins and their strong post-transcriptional regulatory effects.

This book aims to provide a guide to researchers on how to work with Transient Receptor Potential (TRP) channels, describing current methodologies and protocols, and highlighting the challenges ahead that will require the development of new technologies.

Mitogen-activated protein kinase (MAPK) signaling cascades are a group of protein kinases that play a central role in the intracellular transmission of extracellular signals.

This volume presents valuable techniques for studying the class of ligand-activated transcription factors known as nuclear receptors.

Due to their versatility, along with the diminishing costs of library synthesis and the growth of commercial support, peptide microarrays will likely expand beyond being just a research tool into an adaptable and powerful platform to be harnessed for wider drug discovery and point-of-care applications.

Given the versatile utility of the determinination of epitopes, beneficial to a wide variety of scientists from immunologists to structural biologists to biotechnologists, the need for a thorough, state-of-the-art collection of experimental protocols is clear.

The past two decades have seen an explosion in the number of research articles relating to both the physiological and pathological responses evoked by nitric oxide generation.

This book provides a comprehensive overview of and state-of-art information on the production and application of second- and third-generation bioplastics, such as polylactic acid (PLA) and polyhydroxyalkanoates (PHAs).

The book is aimed at providing an exposure to some important topics which are generally not covered adequately in formal courses in biotechnology.

This book is a compendium of knowledge about nanomaterials and strategies for bioremediation over hazardous environmental pollutants.

This book provides a comprehensive overview of and state-of-art information on the production and application of second- and third-generation bioplastics, such as polylactic acid (PLA) and polyhydroxyalkanoates (PHAs).

Abiotic stresses such as drought, high salt, cold, heat, UV radiation, heavy metal pollution, etc.

Abiotic stresses such as drought, high salt, cold, heat, UV radiation, heavy metal pollution, etc.