Biomimetics and Stem Cells: Methods and Protocols collects a series of approaches to demonstrate the role and value of biomimetics for the better understanding of stem cell behavior and the acceleration of their application in regenerative medicine.
This book provides a thorough introduction to widely used techniques for the study of the intersection between developmental biology and neuroscience, an exceptional area to address and investigate impacting biological questions.
This volume provides readers with a historical foundation in standard techniques and a comprehensive update on the latest methods used in making gene-modified mice.
Stem Cell Transcriptional Networks: Methods and Protocols collects techniques used to increase our understanding of the underlying transcriptional programs of stem cells that promote self-renewal and differentiation.
In Animal Models for Stem Cell Therapy: Methods and Protocols, expert researchers in the field detail disease models of hepatic, cardiovascular, neurological diseases, connective and contractile tissue.
This volume explores the diversity in progenitor cell biology methods and uniquely describes techniques for isolating, generating, and characterizing progenitor cells from either tissue or embryonic stem cells.
Updating and building upon previous editions, Hematopoietic Stem Cell Protocols, Third Edition provides up-to-date protocols from leading stem cell researchers.
This book explores the vital importance of T-cell differentiation in areas as wide-ranging as pathological analysis, drug development, and cell therapy of human T-cells.
This updated collection addresses the intense efforts that have been underway to characterize the cellular-molecular-biochemical elements of not only the hematopoietic stem cell niche but the niches for other stem cells.
This volume collects a series of protocols describing the kinds of infrastructures, training, and standard operating procedures currently available to actualize the potential of stem cells for regenerative therapies.
One of the striking findings of modern developmental biology has been the high degree of conservation of signaling and developmental mechanisms amongst different animal species.
Assisted reproductive technologies have had a profound impact on biomedical research through transgenic animals, food supply and production, as wells as genetic gain of domestic species, and treatment of human infertility.
Because of the huge potential of human embryonic stem (hES) cells, especially the newly developed human induced pluripotent stem (hiPS) cells, in disease treatment and life quality improvement, enormous efforts have been made to develop new methodologies to translate lab discoveries in stem cell research into bed-side clinical technologies.
A collection of standard and cutting-edge techniques for using Xenopus oocytes and oocytes/egg extracts to reconstitute biological and cellular processes.
Mesenchymal Stem Cells have seen an unprecedented level of interest in the last decade, primarily due to their relative ease of isolation, the large numbers of cells present in the adult, and the ability to propagate these cells in culture.
Despite political and ethical controversies surrounding the study of human embryonic stem (hES) cells, new freedoms in regard to using them for research has allowed interest to remain high in understanding the regulatory mechanisms of stem cell self-renewal, their differentiation along various lineages, and their potential use in regenerative medicine.
In Developmental Biology of the Sea Urchin and Other Marine Invertebrates: Methods and Protocols, expert researchers in the field detail many of the methods which are now used to study sea urchins and other marine invertebrates in the laboratory.
Due to their vital involvement in a wide variety of housekeeping and specialized cellular functions, exocytosis and endocytosis remain among the most popular subjects in biology and biomedical sciences.
Imaging and Tracking Stem Cells: Methods and Protocols gathers representative protocols related to the vital techniques of stem cell imaging and lineage tracing, including that of live cells, both in vivo and in vitro.
Only in recent times has the possibility of growing and implanting replacement teeth, made from one's own cells, moved into the realm of realistic possibilities; however, the molecular and cellular mechanisms of tooth development must be studied in a range of vertebrates, from zebrafish to mice, so that evolutionarily conserved network kernels, which will define the cellular states of generic vertebrate tooth development, can be recognized.
The germline is unique in mammals as it is the only cell lineage that undergoes mitosis, meiosis, and differentiation, making these cells amenable to in-depth genomic and mechanistic studies.
In Molecular Embryology: Methods and Protocols, Second Edition, expert investigators provide a comprehensive guide to the cutting-edge methods used across the dramatically growing field of vertebrate molecular embryology.
Recent stem cell research has revealed that miRNA and RNAi-mediated gene regulation is one of the vital determinates controlling the state of cell differentiation, with the small RNAs serving as key elements involved in regulatory network control of pluripotent cell fate determination.
Micropropagation is a reliable technology applied commercially worldwide for large-scale plant multiplication, germplasm conservation, pathogen elimination, genetic manipulations and supply of selected plants.
Considerable advances have taken place since the initial isolation and characterization of human embryonic stem (HES) cells; however, significant challenges remain before their potential for restoration and regeneration processes in patients can be realized.
As regenerative medicine involves replacing diseased cells, tissues or organs, or repairing tissues in vivo, the manipulation of stem cells underlie its goals.
Embryonic stem cells (ESCs) offer an unlimited self-renewing capacity, as opposed to the limits of adult stem cells; therefore, ESCs represent an almost bottomless resource for regenerative medicine and tissue engineering approaches.
In the dramatic and rapidly developing field of neural transplantation for CNS repair, the most powerful contributor has been the vital research focusing on stem cells.
Before the therapeutic potential of cell replacement therapy or the development of therapeutic drugs for stimulating the body's own regenerative ability to repair cells damaged by disease and injury can be fully realized, control of stem cell fate, immuno-rejection, and limited cell sources must be overcome.
In recent years, there have been major advances in the concepts and methodologies used in the study of retinal development at both cellular and molecular levels.
Almost daily, new technologies are being presented that move the field of human pluripotent stem cell research towards a future that may yield highly-effective, personalized medical treatments.
Due to its efficacy in animal models, cellular therapy using human hepatocytes is being evaluated worldwide as an alternative to organ transplantation in patients with liver-based metabolic disease and acute liver failure.
The migration of stem cells has been found to be critical during early development for the organization of the embryonic body as well as during adult life with tissue homeostasis and regeneration of organ function.
Stem Cell Nanotechnology: Methods and Protocols gathers several representative protocols related to the emerging interest in nanotechnology as it relates to stem cell biology.
Over time, it has become clear that changes in stem cells do occur during aging, not only in their number but also in their relationship to their microenvironment and their functionality as reflected in changes to their metabolome.
The discovery of stem and progenitor cells in the adult mammalian CNS challenged the long standing "e;no new neuron"e; doctrine and opened the door to the potential for cell replacement therapy.
