Life sciences: general issues

In Flower Development: Methods and Protocols, researchers in the field detail protocols for experimental approaches that are currently used to study the formation of flowers, from genetic methods and phenotypic analyses, to genome-wide experiments, modeling, and system-wide approaches.

Electroporation gene therapy, or gene electrotransfer, has evolved greatly over the last few decades as a result of the remarkable progress in genetic sequencing, gene array analysis, gene cloning, gene expression detection, DNA manufacture and discovery and synthesis of siRNA.

This thorough book provides a collection of techniques used in the emerging field of computational chemogenomics, which is an integration of chemoinformatics, bioinformatics, computer science, statistics, automated pattern recognition and modeling, database usage with data retrieval, and systems integration.

This volume presents a list of cutting-edge protocols for the study of CRISPR-Cas defense systems and their applications at the genomic, genetic, biochemical and structural levels.

Laser Scanning Microscopy and Quantitative Image Analysis of Neuronal Tissue brings together contributions from research institutions around the world covering pioneering applications in laser scanning microscopy and quantitative image analysis and providing information about the power and limitations of this quickly developing field.

This volume describes different computational methods encompassing ligand-based approaches (QSAR, pharmcophore), structure-based approaches (homology modeling, docking, molecular dynamics simulation), and combined approaches (virtual screening) with applications in anti-Alzheimer drug design.

This detailed book contains chapters using unbiased approaches such as chemical and transposon mutagenesis, as well as a protocol for allelic exchange, to make targeted mutants in Staphylococcus, as generating mutants of this bacteria can be accomplished in many ways, depending on the intended changes.

The recent advent of molecular technologies has changed the way plant breeders identify and select their germplasm as genetic variation can now be assessed at the DNA level.

This book presents a comprehensive toolkit of versatile techniques for studying microglia under different experimental settings along with a brief summary of knowledge, accumulated in microglial research over the last decades.

The volume aims at providing an overview of cutting-edge methods and protocols used in neuroscience and neurological research.

This book addresses cutting-edge techniques for researching transposon mutagenesis, an approach for identifying individual gene contributions to the phenotypic characteristics of a particular microorganism.

Molecular Modeling of Proteins, Second Edition provides a theoretical background of various methods available and enables non-specialists to apply methods to their problems by including updated chapters and new material not covered in the first edition.

Mouse Genetics: Methods and Protocols provide selected mouse genetic techniques and their application in modeling varieties of human diseases.

This volume presents methods for the analysis of genomic variability in vertebrate neurons and broadens our knowledge in the ways we understand the brain and its neurons.

This volume includes in-depth, hands-on protocols for detecting various developmental defects in Drosophila.

This second edition volume expands on the previous edition with a look at the latest advances in techniques to study yeast and its core set of interactions, modules, architectures, and network dynamics that are common in all eukaryotes.

This volume explores the recent advancements in biomolecular simulations of proteins, small molecules, and nucleic acids, with a primary focus on classical molecular dynamics (MD) simulations at atomistic, coarse-grained, and quantum/ab-initio levels.

Neurovascular Coupling Methods brings the reader up to date with the current state-of-the-art techniques in measuring blood flow in the brain, with chapters describing different techniques or combinations of techniques, applied to specific species in either healthy or abnormal brains.

Alzheimer's disease (AD) and many other neurodegenerative disorders are multifactorial in nature, involving a combination of genomic, epigenomic, network dynamic and environmental factors.

This thorough book covers the most recent proteomics techniques, databases, bioinformatics tools, and computational approaches that are used for the identification and functional annotation of proteins and their structure.

Exploring the 2-D gel mapping field, the chapters in this book are separated into four different categories: Part I talks about 2-D maps reproducibility and maps modeling; Part II describes the image analysis tools that provide spot volume datasets; Part III is about the statistical methods applied to spot volume datasets to identify candidate biomarkers; and Part IV discusses differential analysis from direct image analysis tools.

This detailed volume will focus on the phenomenon of RNA interference by providing comprehensive coverage of various techniques for in vivo micro/siRNA imaging including the design and synthesis of specific imaging agents and tools, the development of imaging methodologies, and their interpretation.

This volume details computational techniques for analyses of a wide range of biological contexts, providing an overview of the most up-to-date techniques used in the field.

This second edition provides updated and expanded chapters that critically address the issues or rodent stroke modeling, from choosing the model and outcome measures, designing the experiment, conducting and analyzing it, to reporting it in a scientific publication.

This volume provides readers with a comprehensive review of various gene silencing methodologies and its applications.

Rapid changes and significant progress have been made in the Agrobacterium field, such as genetically transforming plants for both basic research purposes and agricultural development.

This volume provides a clear and detailed roadmap of how to design and execute a gene therapy experiment in order to obtain consistent results.

This volume introduces bioinformatics research methods for proteins, with special focus on protein post-translational modifications (PTMs) and networks.

This second edition provides updated and expanded chapters covering a broad sampling of useful and current methods in the rapidly developing and expanding field of bioinformatics.

This volume guides readers through the field of systems medicine by defining the terminology, and describing how established computational methods form bioinformatics and systems biology can be taken forward to an integrative systems medicine approach.

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This volume describes the methods of both in vivo and in vitro electroporation using ferrets, rats, mice, chickens, and zebrafish.

This fully updated book collects numerous data mining techniques, reflecting the acceleration and diversity of the development of data-driven approaches to the life sciences.

This volume explores the latest updates on microscopy approaches and techniques used by scientists studying in the field of gene expression imaging.

This volume details several important databases and data mining tools.

This volume describes techniques to make synaptosomes, and discusses numerous ways that synaptosomes are used to study and answer diverse questions about synapses.

This volume discusses the latest techniques and methodologies used in the neurotrophin field to study the physiology of the Brain-Derived Neurotrophic Factor (BDNF).

This volume details methods that will aid in the selection of promoter sequences and vector components and methods for the assembly and testing of synthetic promoters with examples of their application.

This volume focuses on methodological aspects of systems biology in order to provide a new theoretical approach with a robust and tailored experimental support.

This second edition provides updated and expanded chapters covering a broad sampling of useful and current methods in the rapidly developing and expanding field of bioinformatics.

To foster a better understanding of dopamine receptor functionality, this detailed volume creates an interface between updated classical methods and new emerging technologies heretofore not available to new or seasoned researchers.

This volume represents a valuable and readily reproducible collection of established and emerging techniques for neuronal cell death research.

This detailed volume collects protocols for experimentation into how neurons connect to produce the extraordinary functionalities of the nervous system.

This volume presents the latest methods and approaches used to better characterize the properties of the structural human brain, and also explores various dysfunctional and non-dysfunctional neuroanatomical variations.

The second edition of this volume provides up-to-date methods on the main methodological aspects of functional MRI (fMRI), applying fMRI to the study of central nervous system, and future evolutions of fMRI techniques.

This second edition volume expands on the previous edition with updates on the latest techniques used in the field of proteomics.

This book provides a comprehensive, interdisciplinary collection of the main, up-to-date methods, tools, and techniques for microarray data analysis, covering the necessary steps for the acquisition of the data, its preprocessing, and its posterior analysis.

This volume provides a collection of protocols for the study of DNA-DNA contact maps, replication profiles, transcription rates, RNA secondary structures, protein-RNA interactions, ribosome profiling and quantitative proteomes and metabolomes.

This volume examines different approaches toward understanding the function of microRNA (miRNA) and the tools used to regulate microRNA expression in nervous systems.

This volume on metabonomics provides detailed information on the procedures involved in nuclear magnetic resonance (NMR) spectroscopy, gas chromatography-mass spectrometry (GS-MS), liquid chromatography-mass spectrometry (LC-MS), and capillary electrophoresis-mass spectrometry (CE-MS).