This volume is directed at individuals interested in the field of neuroscience who are novices or experts in the use of transmission electron microscopy.
This volume provides current methods to analyze the properties of designer receptors exclusively activated by designer drugs (DREADDs) in vitro and to measure the biological responses of DREADD activation in different neuronal populations in vivo.
This book provides methods and clear protocols for the various technologies available to detect, characterize, and study Citrus tristeza virus (CTV), a member of the genus Closterovirus, family Closteroviridae.
This book provides definitive methods to perform stable isotope probing (SIP) experiments, covering a wide spectrum of stable isotope techniques used in microbial ecology, such as methods to target and analyze labeled DNA, rRNA, mRNA, protein, and PLFA.
This thorough volume provides an in-depth introduction to and discussion of microRNAs (miRs) and their targets, miR functions, and computational techniques applied in miR research, thus serving the need for a comprehensive book focusing on miR target genes, miR regulation mechanisms, miR functions performed in various human diseases, and miR databases/knowledgebases.
This fully updated second edition explores protocols that address the most challenging aspects of experimental work in ancient DNA, such as preparing ancient samples for DNA extraction, the DNA extraction itself, and transforming extracted ancient DNA molecules for sequencing library preparation.
This volume offers detailed step-by-step instructions to allow beginners and experts alike to run appropriate copy number variants (CNV) detection software on a dataset of choice and discern between false positive noise and true positive CNV signals.
Receptor and Ion Channel Detection in the Brain provides state-of-the-art and up-to-date methodological information on molecular, neuroanatomical and functional techniques that are currently used to study neurotransmitter receptors and ion channels in the brain.
This book provides current glycoinformatics methods and protocols used to support the determination of carbohydrate structures in biological samples as well as carbohydrate structure databases, the interaction of carbohydrates with proteins, and theoretical and experimental methods to study their three-dimensional structure and dynamics.
This book brings together recent methods and theoretical approaches developed to dissect the activity and function of bacterial and eukaryotic Structural Maintenance of Chromosome (SMC) proteins.
This volume covers various aspects of co-immunoprecipitation (co-IP) methods and its relevant use in studying protein-protein interactions (PPIs) in health and diseases of the Central Nervous System.
This book focuses on recent developments in docking simulations for target proteins with chapters on specific techniques or applications for docking simulations, including the major docking programs.
This volume provides an overview of RNA bioinformatics methodologies, including basic strategies to predict secondary and tertiary structures, and novel algorithms based on massive RNA sequencing.
This volume aims to explore the latest developments in adeno-associated viral and lentiviral vectors as well as the gene therapy strategies for the most common neurological disorders, followed by chapters that include step-by-step guides to viral vector-based gene delivery in animal models used in the authors' laboratories.
This detailed volume gathers together a broad variety of methods essential to the investigation of the biology of astrocytes and their multifaceted roles in both healthy and diseased brains.
Yeast Metabolic Engineering: Methods and Protocols provides the widely established basic tools used in yeast metabolic engineering, while describing in deeper detail novel and innovative methods that have valuable potential to improve metabolic engineering strategies in industrial biotechnology applications.
This volume collects protocols and procedures utilizing cellular, tissue, and whole animal models that can be applied to the investigation of neurotrophic factors and other agents impacting on these systems.
As autism is associated with many rare diseases, this extensive bench book examines how no one single model can be used; multiple organism models are needed, each one corresponding to one of the diseases or to one aspect of a disease.
In Clinical Bioinformatics, Second Edition, leading experts in the field provide a series of articles focusing on software applications used to translate information into outcomes of clinical relevance.
This volume discusses the importance of imaging, fluid, and genetic biomarkers in detecting the earliest pre-symptomatic stages of Alzheimer's disease (AD).
This volume covers methodologies, ranging from the molecular level to the network level, used to study receptor-receptor interactions in heteroreceptor complexes inside the central nervous system.
This detailed volume reflects the considerable new developments that have taken place in the area of applied neurogenomics, particularly with the rapid progress in next generation sequencing.
This volume focuses on various approaches to studying long non-coding RNAs (lncRNAs), including techniques for finding lncRNAs, localization, and observing their functions.
Quantitative Real-Time PCR: Methods and Protocols focuses on different applications of qPCR ranging from microbiological detections (both viral and bacterial) to pathological applications.
This volume explores the latest available wet-lab techniques and computational methods to study in-cell small-molecule behavior and interactions with their targets.
This book provides both historical perspective and practical information to support researchers either currently involved in genomic research on rats or planning to begin such a project.
This volume provides readers with an introduction to all the methods and major approaches now being used to study the structural and functional development of the brain.
This volume provides methods for characterization of muscarinic receptor in crystallography studies that advanced our understanding of structural properties and activation mechanism of muscarinic receptors and are cornerstone in molecular modeling and computer based approaches to study muscarinic receptors.
This detailed volume assembles comprehensive protocols to assist with the study of structural, molecular, cell biological, and in vivo facets of GPCRs, and to enable the development of experimental tools for screening novel GPCR drugs.
This detailed book provides a laboratory manual and guidebook for the selection, implementation, and interpretation of a wide range of techniques in contemporary use in leading laboratories engaged in Huntington's disease (HD) research worldwide.
Providing a spectrum of models that is reflective of the various species that can be utilized in experimentation on disorders across a broad range of developmental disabilities, this volume collects expert contributions involved in investigation of the causes, outcomes, treatment, and prevention.
