This collection explores detailed experimental protocols necessary for setting up a variety of in vitro cytochrome P450 (CYP) assays that are vital in selecting drug candidates in a drug discovery pipeline.
This book serves as a guide for identifying and applying commonly used cell-based translational assays as well as for assessing the therapeutic potential of new immuno-oncology therapeutics and advancing their mechanism of action.
This detailed book explores protocols with the aim of testing aerobic exercise, resistance training, special diets, additives and natural products, which have led to new insights into the physiological and molecular aspects of health and disease.
This fully updated volume presents a wide range of methods for synthesis, surface modification, characterization and application of nano-sized materials (nanoparticles) in the life science and medical fields, with a focus on drug delivery and diagnostics.
This book focuses on ion mobility-mass spectrometry (IM-MS) and informatics approaches to improve traditional analysis of molecules by providing fundamentals and protocols for exploiting the potential of state-of-the-art IM-MS technology for the most common analytical applications.
This book highlights plastic pollution, especially microplastics, and the consequences on the environment and on the human diet through food chain/web transmission and contamination through processing and packaging of various human food items including water and beverages.
This detailed volume covers conventional MS-based "e;shotgun lipidomics"e; by which samples are introduced by infusion or loop injection, as well as LC-MS-based lipidomics, which are becoming increasingly important due to the ever-increasing demand for a complete and precise lipid analysis of the complex and diversified lipids in nature.
This book provides a comprehensive overview of the major biochemical analytical techniques, with detailed descriptions of the instrumentation and applications.
This second edition volume expands on the previous edition with updated research and techniques to help laboratory workers design and implement a successful purification strategy, emphasize critical aspects on practical problems, and answers questions encountered at the lab bench.
This fully updated edition targets not only those assays directly involved in the discovery of GPCR-active compounds but also those involved in cell-based experiments designed to study physiological responses.
This volume focuses on computational modeling of the ecotoxicity of chemicals and presents applications of quantitative structure-activity relationship models (QSARs) in the predictive toxicology field in a regulatory context.
This volume presents methods used for the analysis of glycoproteins at different levels-intact, subunit, glycopeptide, and monosaccharide--, and discusses and solves most analytical challenges that a scientist working on glycoproteins may come across.
This thorough book explores some of the most important methods and concepts affecting the quantitative analysis of the transport, targeting, and disposition of chemicals within cells, which in turn impact the macroscopic pharmacokinetics of chemical agents in the whole organism.
This book aims to provide a guide to researchers on how to work with Transient Receptor Potential (TRP) channels, describing current methodologies and protocols, and highlighting the challenges ahead that will require the development of new technologies.
The aim of this edition is to introduce the beginner to the basics of affinity chromatography and provide practical knowledge for the development of affinity separation protocols.
This detailed book focuses on analytical separations by chromatographic and electrophoretic techniques, providing some overview along with numerous practically-oriented applications of the most important analytical techniques in chiral separation sciences.
This second edition volume expands all chapters of the previous edition, which have been enhanced to cover the most recent developments, the current state of method research, and applications.
This volume describes prominentmethodologies developed by laboratories that have been leading the field ofquantitative proteomics by mass spectrometry.
This volume presents new developments in the field of mass spectrometry imaging, covering imaging, software, data analysis, new instrumentations, and new methodological approaches.
This book provides current glycoinformatics methods and protocols used to support the determination of carbohydrate structures in biological samples as well as carbohydrate structure databases, the interaction of carbohydrates with proteins, and theoretical and experimental methods to study their three-dimensional structure and dynamics.
This book covers strategies to improve cell permeability, intestinal permeability, and metabolic stability, which are the typical liabilities associated with cyclic peptides, to enhance protein-protein recognition, and to build upon nature's cyclic peptides and macrocycles.
This book explores cutting-edge methods to work with the notoriously difficult, but highly prevalent, obligate intracellular pathogen, Chlamydia trachomatis.
This book highlights the potential of e-device technology to serve as a successful platform for multiplexed sensing, along with the methods for device fabrication, calibration, and assays in multiple applications.
This volume covers the structures, properties, and functions of G-quadruplexes in a wide range of biological disciplines, including therapeutic intervention and biomaterial application.
This volume explores techniques used to detect lipids attached to proteins, to analyze the function of lipid modifications, and to characterize the enzymes that add and remove lipids from proteins.
This volume details protocols on formulation, surface modification, characterization, and application of a variety of pharmaceutical nanocarriers such as micelles, nanoparticles, dendrimers, carbon dots, polymersomes, and others.
The aim of this new edition is to provide detailed information on each topic and present novel ideas and views that can influence future developments in mass spectrometry-based proteomics.
This detailed book explores techniques commonly used for research into drug repurposing, a well-known strategy to find alternative indications for drugs which have already undergone toxicology and pharma-kinetic studies but have failed later stages during the development, via computational methods.
This third edition volume expands on the previous editions with new topics that cover drug discovery through translational bioinformatics, informatics, clinical research informatics, as well as clinical informatics.
New insights into modern medicine and systems biology are enabled by innovative protocols and advanced technologies in mass spectrometry-based proteomics.
This comprehensive volume collects a repertoire of techniques for the adoption and exploitation of RNA interference (RNAi) as a fertile strategy to develop the bio-drugs of future in the vital field of cancer research.
This book provides broad coverage of nuclear magnetic resonance (NMR) spectroscopy-based methods and applications for the analysis of metabolites in a wide range of biological samples, from biofluids, cells, animal models, human, to plants and foods.
The volume brings together some of the best experts in the field of modern metabolomics to discuss various techniques used today to study specific metabolite classes, and metabolomics in bacterial systems and mammalian systems.
If the development of techniques for the labeling of a polypeptide- terminus and for repetitive N-terminal sequencing mark the beginning of the science of protein structure determination, then the field has just about reached its half century.
