Over the last 15 years, there has been renewed interest in supercritical fluids owing to their unique properties and relatively low environmental impact.
Driving further the research on mammalian alkaline phosphatase structure and function, Phosphatase Modulators collects expert contributions into one "e;how to"e; manual for basic scientists interested in initiating a drug discovery effort.
Over the past years, the chem(o)informatics field has further evolved and new application areas have opened up, for example, in the broadly defined area of chemical biology.
The advent of non-invasive imaging technology, such as magnetic resonance imaging (MRI), has allowed biologists and clinicians to make great strides in unraveling the secrets of the brain.
Due to the significant contributions of carbohydrates to the functional diversity of the cell, the challenging study of the glycome has expanded beyond the research of carbohydrate experts and into the wider scope of the life sciences.
Proteins are the functional units of the cellular machinery and they provide significant information regarding the molecular basis of health and disease.
SELDI is distinct from other TOF-MS technologies in that it couples features of chromatography and mass spectrometry, facilitating analyte enrichment and sample cleanup on an array surface.
As the emerging field of proteomics continues to expand at an extremely rapid rate, the relative quantification of proteins, targeted by their function, becomes its greatest challenge.
Drug research has been greatly transformed by the "e;omics revolution"e; and advances in computational tools, combinatorial chemistry, and high throughput screening techniques (HTS).
With significant developments in the areas of chromatography and spectroscopy as well as the unique inherent chemical diversity of natural products, vital in drug research, natural products research has gained new momentum.
Since each human is genetically distinctive, responding differently to disease-causing factors as well as drugs, the field pharmacogenomics arose to develop personalized medicine, or medicine that deals with the complexity of the human body.
Expanding upon the research elucidated by the first volume of this collection, Advanced Protocols in Oxidative Stress II presents thirty additional cutting-edge chapters focusing on novel techniques for detecting ROS/RNS, unique AOX technology and applications, gene expression and biostatistics for evaluating OS-derived experimental data.
Capillary electrophoresis (CE) is a powerful and rapid tool for performing complex analyses of a number of different molecular species ranging from small inorganic ions to large nucleic acid fragments and proteins.
Glycosyltransferases (GTs) are essential for the biosynthesis of complex glycoconjugates and are powerful tools to study the functions of complex glycans in health, development and disease.
There is a demand for analytical methods that are able to discriminate between enantiomers in order to analyze the enantiomeric purity of compounds from natural or chemical sources not only in pharmaceutical sciences but in any field on bioactive compounds including chemistry, biology, biochemistry, forensic, and environmental sciences and many others.
As mass spectrometric methods now offer a level of specificity and sensitivity unrealized by spectrophotometric- and immunoassay-based methods, mass spectrometry has entered the clinical laboratory where it is being used for a wide range of applications.
The field of protein NMR spectroscopy has rapidly expanded into new areas of biochemistry, molecular biology and cell biology research that were impossible to study as recently as ten years ago.
Classical natural product chemistry is transitioning to modern day metabolomics as a result of the advent of comprehensive analytical platforms and sensitive analytical instrumentation.
Capillary electrophoresis (CE) is a relatively new separation technique suitable for handling small amounts of sample very important in bioanalytical research and in various clinical, diagnostic, genetic, and forensic applications.
The study of functional glycomics requires the continuous development of rapid and sensitive methods for the identification of glycan structures and integration to structure-function relationships.
With the development of new quantitative strategies and powerful bioinformatics tools to cope with the analysis of the large amounts of data generated in proteomics experiments, liquid chromatography with tandem mass spectrometry (LC-MS/MS) is making possible the analysis of proteins on a global scale, meaning that proteomics can now start competing with cDNA microarrays for the analysis of whole genomes.
Microtubules are essential components of the cytoskeleton, and play critical roles in a variety of cell processes, including cell shaping, intracellular tracking, cell division, and cell migration.
Now a routine tool in biomedical and life science research, live cell imaging has made major progress enabling this core biochemical, cell, and molecular biology technique to become even more powerful, versatile, and affordable.
Forensic DNA profiling procedures are mainly based on high resolution and high throughput capillary electrophoresis separation and detection systems of PCR amplicons obtained from DNA genomic markers with different inheritance patterns.
The last decade has seen the confluence of several enabling technologies that have allowed protein crystallographic methods to live up to their true potential.
Despite being known and studied for years, peptides have never before attracted enough attention to necessitate the invention of the term "e;peptidomics"e; in order to specify the study of the complement of peptides from a cell, organelle, tissue or organism.
Since the publishing of the first edition, the methodologies and instrumentation involved in the field of mass spectrometry-based proteomics has improved considerably.
Chemogenomics aims toward the systematic identification of small molecules that interact with the products of the genome and modulate their biological function.
Reflecting the expanding field's need for reliable protocols, Fluorescence Spectroscopy and Microscopy: Methods and Protocols offers techniques from a worldwide team of experts on this versatile and vital subject.
In DNA Electrophoresis: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study DNA using electrophoresis as the major approach.
Leading chemists and engineers concisely explain the principles behind microchip capillary electrophoresis and demonstrate its use in a variety of biochemical applications, ranging from the analysis of DNA, proteins, and peptides to single cell analysis and measuring the impact of surface modification on flow in microfluidic channels.
