Diseases and disorders

This volume provides readers with methods and protocols for understanding the development of recombinant viruses and their use as vaccines platforms.

Psychoneuroimmunology (PNI) has developed rapidly in the last four decades.

Cancer Vaccines: Methods and Protocols explores the manipulation and modification of immune cells, the manipulation and modification of tumor cells as well as the manipulation of immune/tumor interactions and various delivery mechanisms, with the overall end goal of evoking a tumor-specific response and overcoming the immuno-evasive mechanisms employed by the tumor cells.

This book provides a practical guide to current methods for profiling and interpreting genomic alterations in tumors.

Our understanding of the apoptotic pathway and its regulation has improved significantly in the last 10 years.

This volume details common experimental approaches in studies designed to illuminate various processes used to study cancer metabolism.

Target discovery is a field that has existed for several years but is so vibrant today because of the recent progress in our understanding of the molecular mechanisms of many human diseases and the technical advances in target identification and validation.

Continued refinement of wide-spread access to transgenic technology has allowed for new animal models have been developed that exhibit features of autoimmune disease have been developed that exhibit features of autoimmune disease.

This second edition provides updated and new chapters on T-Cell trafficking.

Leading clinicians and scientists in solid organ transplantation review the current status of the field and describe cutting-edge techniques for detecting the immune response to the allografted organ.

Cancer Cell Signaling: Methods and Protocols, Second Edition brings together the most recent advances in cancer cell signaling knowledge along with recent progress in the development of novel strategies and experimental cell models to study human cancer.

This volume provides detailed descriptions of prevailing and novel techniques used by experts in the study of PTEN function in disease and biology.

This second edition volume expands on the previous edition with a discussion of new and updated methods used to study the Herpes Simplex Virus (HSV), along with a look at the latest developing technologies such as next generation sequencing, CRISPR/Cas9 engineering, and the use of BioID to identify protein-protein interactions.

This volume explores disease diagnosis and therapy in developing theranostics.

Infection by flaviviruses such as dengue virus serotypes (DENV 1-4), Japanese encephalitis virus (JEV), tick-borne encephalitis virus (TBE), yellow fever virus (YFV) and West Nile virus (WNV) impact millions of lives and cause tens of thousands of mortalities each year.

In Apoptosis and Cancer: Methods and Protocols, Second Edition, expert researches in the field detail the performance of molecular and cellular biology techniques for studying and detecting the activation of the apoptotic pathway.

This detailed volume gathers a comprehensive collection of methods, protocols, and procedures used for the identification, characterization, and selection of cancer stem cells.

More than ever, antibodies are being recognized as a major drug modality in a variety of diseases, including cancer, autoimmune diseases, infectious diseases, or even neurodegenerative disorders.

Despite major advances in HIV treatment, many areas require more study, in order to create efficacious, potent antiretrovirals that can suppress viral load completely and durably without toxic side effects, to define unknown drug targets and fine-tune known targets, and to better understand the interplay between viral and host factors.

Prion Protein Protocols brings together a collection of current protocols in the field of mammalian prion disease research.

The epigenetic regulation plays an important role in normal development and maintenance of tissue specific genes expression in humans and the disturbance of these patterns lead to changes involved in tumor formation.

According to the most recent clinical oncology data, one out of seven newly diagnosed malignancies worldwide result from infection and chronic inflammation in conjunction with cancer.

Population studies and epidemiology facilitate the discovery of genetic and environmental determinants of cancer and the development of new approaches to cancer control and prevention, therefore they play a central role in the creation of health policies.

Neisseria meningitidis is a major causative agent, worldwide, of potentially life-threatening meningitis and septicaemia, which carry with them a high mortality rate and permanent physical and neurological issues for survivors.

Intracellular checkpoint controls constitute a network of signal transd- tion pathways that protect cells from external stresses and internal errors.

The innate immune system represents a critical arm of the immune response by providing immediate and robust host defense; however, human studies of its function are often limited by ethical, logistical, and technical obstacles.

Enzyme-linked immunospot assay (ELISPOT) has been known for some time as a unique state-of-the-art technique for studying the cytokine-secreting activity of immune system cells, and it appears to be one of the fast growing applications in biomedical research, becoming an indispensable tool in vaccine development, HIV research, transplantation studies, and cancer and allergy research.

The avian influenza (AI) virus, which is the type A influenza virus adapted to an avian host, has been an important pathogen for the poultry industry worldwide for many years.

Populations of the western world are now healthier and enjoying higher life expectancy than ever.

The development of the hybridoma technology created the possibility to obtain unlimited amounts of monoclonal antibodies (mAb) with high specificity and affinity for any target and to introduce mAbs in a wide range of applications; however, the bulky size of mAbs, costly production, and cumbersome engineering hampered regularly their streamlined development in some applications.

In addition to their invaluable role in the fight against infection, Toll-Like Receptors (TLRs) in an under- or over-active state can lead to the pathogenesis of disease, making these receptors a key focal point for many research laboratories.

Glycovirology Protocols reviews the increasing importance of glycosylation to the field of virology, as well as virus replication.

This book presents a comprehensive toolkit of versatile techniques for studying microglia under different experimental settings along with a brief summary of knowledge, accumulated in microglial research over the last decades.

This book provides the immune oncology (IO) community with a deeper understanding of the scope of the biomarker methods to potentially improve the outcome from immunotherapy.

Thisvolume summarizes current techniques; ranging from protocols for virus growth,isolation, quantification and generation of recombinant RSV virus to proceduresfor the efficient characterization of the host immune response to RSVinfection.

Cancer is a devastating disease that affects millions of people in the world.

Over 2000 years ago in China, antibodies elicited by early forms of vaccination likely played a major role in the protection of the population from infectious agents.

A collection of state-of-the-art molecular methods for studying antifungal resistance, for discovering and evaluating both new and existing antifungal drugs, and for understanding the host response and immunotherapy of such agents.

A compendium of readily reproducible and novel methods to manipulate DNA viruses and characterize their varied biological properties.

Over the last few years, the methods for analyzing cancer-related genes on a molecular level have changed rapidly.

Recent advances in molecular and cellular biology techniques have significantly improved our ability to detect, monitor, model and study the underlying molecular basis and pathogenesis of leukemia, yet we are still in an early discovery stage and much more work is needed in order to develop better strategies to diagnose, classify and treat this biologically and clinically diverse disease.

A compendium of optimized methods to measure type I interferon efficacy as an antiproliferative or an antiviral agent.

In this first book dedicated entirely to the ELISPOT, a critical enzyme-linked immunospot assay used widely in biomedical research, recognized experts with first-hand experience detail how to design, perform, and analyze these assays.

Antigen processing is a biological process that prepares antigens for the presentation to special cells in the immune system called T lymphocytes.

This third edition volume expands upon the previous editions with new research topics and numerous leading-edge HIV protocols.

This volume presents a comprehensive collection of methods that have been instrumental to the current understanding of bacterial persisters.

In the last decade, a large number of major discoveries have shed light on the molecular mechanisms of lymphocyte migration and the anatomy of immune responses.

Due to their vital involvement in a wide variety of housekeeping and specialized cellular functions, exocytosis and endocytosis remain among the most popular subjects in biology and biomedical sciences.

Inflammasomes have a seemingly simple architecture.