Pre-clinical medicine: basic sciences

Recent insight into the transcripts generated from the mammalian genome (i.

This book sheds new light on the history of exercise physiology and how it essentially grew, thanks to the work of a few major Schools.

As experimentation and clinical trials with first generation anti-angiogenic agents have yielded results and our understanding of the biology and physiology of blood and lymphatic vessels has increased, a new angiogenesis volume swiftly became a necessity.

This detailed book encapsulates the most up-to-date methods of the intestinal stem cell field and provides guidance on a variety of techniques for studying intestinal stem cells properties.

In Situ Hybridization Protocols, Fourth Edition contains 21 protocols that utilize the in situ hybridization technology to document or take advantage of the visualization of specific RNA molecules.

This detailed volume focuses on population epigenetics, which is of increasing interest to policy makers searching for explanations for complex epidemiological observations and conceptual models on which to base interventions.

Continuing the research of the best-selling first edition, Patch-Clamp Analysis: Advanced Techniques, Second Edition collects three more years of research in the ever-expanding study of the cell membrane.

In this new volume, renowned authors contribute fascinating, cutting-edge insights into microarray data analysis.

Gene therapy offers many conceptual advantages to treat muscle diseases, especially various forms of muscular dystrophies; however, it faces a number of unique challenges, including the need to deliver a therapeutic vector to all muscles throughout the body.

This reference book provides a comprehensive overview of natural gums, resins, and latexes of plants with a focus on their chemistry, biological activities, and practical uses.

This book brings together lessons learned in the clinical use of anorectal endosonography and manometry in paediatrics.

The purpose of this book is to highlight some of latest developments and applications of CRISPR, RNA, and DNA to treat diseases ranging from cancers to cardiovascular and degenerative disorders.

Since the publication of the first edition, the techniques and experimental approaches employed in the study of adipose tissue have undergone radical expansion and revision.

In Gene Therapy Protocols, Volumes 1 & 2, internationally recognized investigators describe cutting-edge laboratory techniques for the study of Production and In Vivo Applications of Gene Transfer Vectors (Volume 1) and Design and Characterization of Gene Transfer Vectors (Volume 2).

Bacterial Artificial Chromosomes, Second Edition expands upon the previous edition with current, detailed methods developed for working with BACs.

Gene expression can mean the difference between a functional and non-functional genome, between health and disease, and with the development of transgenic crops, the difference between survival and starvation.

Stem cells are responsible for maintaining tissue homeostasis.

Providing a spectrum of models that is reflective of the various species that can be utilized in experimentation on disorders across a broad range of developmental disabilities, this volume collects expert contributions involved in investigation of the causes, outcomes, treatment, and prevention.

This book, built on the latest internationally recognized classification system to which the volume editor contributed, offers a unique and comprehensive reference guide to the clinical presentation, characteristics and proper treatment of the wide spectrum of congenital malformations of the inner ear (IEM).

This third edition provides new and updated chapters on design PCR primers for successful DNA amplification.

This book provides a collection of comprehensive, up-to-date, and broadly applicable guides to the research and development fields of oligonucleotide (ON) therapeutics.

This volume provides readers with a historical foundation in standard techniques and a comprehensive update on the latest methods used in making gene-modified mice.

This new edition explores current and emerging mutagenesis methods focusing specifically on mammalian systems and commonly used model organisms through comprehensive coverage and detailed protocols.

This detailed volume explores key technologies that are used currently to investigate iGluR structure, function and physiology.

Featuring current resources used to discover new legume family genes and to understand genes and their interactions, Legume Genomics: Methods and Protocols provides techniques from expert researchers to study these plants that are so vitally important for food, feed, human nutrition, bioenergy, and industrial purposes.

This contributed volume discusses the most important physiological aspects of phosphate metabolism, and how its pathological dysregulation can induce organ damage, which includes but is not limited to blood vessels, kidney, bone and tumor.

Cardiac Gene Expression: Methods and Protocols presents both cutting-edge and established methods for studying cardiac gene expression.

Forensic DNA profiling procedures are mainly based on high resolution and high throughput capillary electrophoresis separation and detection systems of PCR amplicons obtained from DNA genomic markers with different inheritance patterns.

Molecular biology has driven a powerful reductionist, or "e;molecule-c- tric,"e; approach to biological research in the last half of the 20th century.

Ever since their discovery more than 150 years ago, astrocytes have proved to be something of an enigma.

Advances in methodologies and experimental models are pivotal to furthering our understanding of central nervous system (CNS) functions in mammals.

Neuroproteomics: Methods and Protocols presents experimental details for applying proteomics to the study of the central nervous system (CNS) and its dysfunction through trauma and disease.

The huge potential for gene therapy to cure a wide range of diseases has led to high expectations and a great increase in research efforts in this area, particularly in the study of delivery via viral vectors, widely considered to be more efficient than DNA transfection.

Hox Genes: Methods and Protocols explores techniques and methodologies which arose from or were successfully applied to the study of Hox genes and Hox proteins, at the intersection of experimental embryology, genetics, biochemistry, physiology, evolutionary biology and other life sciences.

In the post-genomic era, in vitro mutagenesis has emerged as a critically important tool for establishing the functions of components of the proteome.

Recent work has revealed that stabilizing G-quadruplexes in telomeric DNA inhibits telomerase activity, providing impetus for the development of G-quartet-interacting drugs, while G-quartet-containing oligonucleotides have been recognized as a potent class of aptamers effective against STAT3 and other transcription factors implicated in oncogenesis, proving these guanine-quartets to be a vital and rich area for future study.

This volume is a collection of miRNA detection and target identification protocols, detailing new developments in the traditional detection approaches such as northern blot, quantitative real-time PCR, array, next generation sequencing, and in situ hybridization.

The aim of this volume is to provide a step-by-step guide for implementing a selection of novel techniques in the lab.

Stem cells and regenerative medicine is a fast emerging field with rapid strides of progress and focus on human health.

New and exciting biological functions are still being discovered for vitamin A derivatives, including the vast number of physiological activities of retinoids.

Chromosomes, as the genetic vehicles, provide the basic material for a large proportion of genetic investigations, from the construction of gene maps and models of chromosome organization, to the inves- tigation of gene function and dysfunction.

Understanding an individual's genetic makeup is the key to creating personalized drugs with greater efficacy and safety, and pharmacogenomics aims to study the complex genetic basis of inter-patient variability in response to drug therapy.

This 2nd edition of the book on DNA methyltransferases has been comprehensively updated to reflect many novel research findings regarding the structure, function, and technology of these enzymes that have emerged over the past 6 years.

Once a tedious, highly skilled operation, reverse-transcription polymerase chain reaction (RT-PCR) has become a routine and invaluable technique used in most laboratories.

This volume thoroughly describes the fundamentals of a new multidisciplinary field of study that aims to deepen our understanding of the human body by combining medical image processing, mathematical analysis, and artificial intelligence.

Providing a list of methods useful both to those who wish to study pseudogenes and to those who actually want to avoid their inadvertent detection, Pseudogenes: Functions and Protocols explores techniques involving pseudogenic DNA, RNA, and peptides/proteins, once believed to lack any functionality, but now known to be involved in complex regulatory circuits.

The book focuses on describing the importance of genetics in eye diseases in a comprehensive and concise manner.

In RNA Mapping- Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used to study RNA.