Genetics (non-medical)

In Flower Development: Methods and Protocols, researchers in the field detail protocols for experimental approaches that are currently used to study the formation of flowers, from genetic methods and phenotypic analyses, to genome-wide experiments, modeling, and system-wide approaches.

This detailed collection explores genome-wide association studies (GWAS), which have revolutionized the investigation of complex traits over the past decade and have unveiled numerous useful genotype-phenotype associations in plants.

This volume shares technologies that detect common epigenetic changes which are very important in the early detection, progression, and prognosis of cancer as well as the design of new therapeutic tools against cancer cells.

This volume opens by covering two main types of approaches widely used to determine essential genes: single-gene knockouts and transposon mutagenesis, in both prokaryotes and Candida albicans.

This second edition provides new and updated chapters detailing recent advances in MYC research and current techniques.

This completely revised edition explores novel discoveries in bacterial genomic research, with a focus on technical and computational improvements as well as methods used for bacterial pangenome analysis, which relies on microbiome studies and metagenomic data.

This book expands upon the useful first edition by exploring classic Quantitative Polymerase Chain Reaction (qPCR) techniques as well as a number of recently developed applications.

This detailed volume provides a collection of protocols for the study of miRNA functions in plants.

This volume provides detailed descriptions on cutting-edge experimental methods to assess the multiple and complex interactions between T.

This book provides numerous methods for identification, validation, and functional characterization of chimeric RNAs, herein described as any transcript which contains the nucleotide sequence of two distinct parental genes.

This book provides methods and techniques used in construction of global transcriptional regulatory networks in diverse systems, various layers of gene regulation and mathematical as well as computational modeling of transcriptional gene regulation.

Mouse Genetics: Methods and Protocols provide selected mouse genetic techniques and their application in modeling varieties of human diseases.

This volume contains cutting-edge techniques to study the function of enhancers and promoters in depth.

In both vertebrates and invertebrates, Hedgehog signaling regulates the development of the tissues and structures of the body.

Transposable elements have played a major role in shaping plant genome structure and gene expression.

This fully revised book includes new and improved protocols to analyze toxicity at the DNA, RNA, and protein levels using genomic, epigenomic, transcriptomic, and proteomic approaches.

Plant-Pathogen Interactions: Methods and Protocols provides key methods, approaches, and strategies to dissect the plant defense response.

Arabidopsis Protocols, Third Edition compiles some of the most recent methodologies developed to exploit the Arabidopsis genome.

Plants come in myriads of shapes and colors, and the beauty of plants has fascinated mankind for thousands of years.

Structural genomics is a newly emerging field that has arisen following the successful footsteps of the major sequencing efforts generally bundled under the heading "e;genomics"e;.

RNA abundance analysis is one of the most important approaches for gene expression studies in the field of molecular biology.

A DNA barcode in its simplest definition is one or more short gene sequences taken from a standardized portion of the genome that is used to identify species through reference to DNA sequence libraries or databases.

Given the popularity and utility of Saccharomyces cerevisiae, yeast-based functional genomics and proteomics technologies, developed over the past decade, have contributed greatly to our understanding of bacterial, yeast, fly, worm and human gene functions.

Cytogenetic studies of malignancy have become an essential tool in the clinical management of cancer patients.

After the generation of genome sequence data from a wide variety of plants, databases are filled with sequence information of genes with no known biological function, and while bioinformatics tools can help analyze genome sequences and predict gene structures, experimental approaches to discover gene functions need to be widely implemented.

Metagenomics has proven to be a powerful tool for exploring the ecology, metabolic profiling, and comparison of complex microbial communities as well as its important applications in the mining of metagenomes for genes encoding novel biocatalysts and drug molecules for bioindustries.

RNA interference has become a key method in the suppression of gene expression and the development of therapeutic agents, yet there is still the problem of delivery, stability, and the danger of off-target effects such as the silencing of unwanted genes and activation of innate immunity.

The numerous vital applications of complementary DNA (cDNA) technology have changed dramatically as the technology has advanced over recent years.

Phytoplasmas are a group of bacteria that are capable of multiplying and causing severe diseases in a wide range of plant species and are transported between plants by insect vectors in which the bacteria can also multiply.

This volume focuses on various approaches to studying long non-coding RNAs (lncRNAs), including techniques for finding lncRNAs, localization, and observing their functions.

The rapid pace of microRNA (miRNA) research continues to drive the advances of techniques for miRNA expression profiling, and innovative technologies that are more sensitive, specific, quantitative, and that are compatible with a wide range of biospecimens have been developed during the past few years.

Two decades have passed since trinucleotide repeat expansion was first discovered in genes responsible for certain neurological diseases.

Central to the synthesis of proteins, the performance of catalysis, and many other physiological processes, the aberrant expression of which can be linked to human diseases including cancers, RNA has proven to be key target for therapeutics as well as a tool for therapy.

As increasing global population and continuing economic development ensure the need for further production and cultivation of maize, the necessity of the application of transgenic technology to this model species and crop plant grows steadily.

The book focuses on the regulation of protein function and biological activity by the post-translational modification known as acetylation.

Despite the best efforts of many and despite landmark discoveries and experimental ingenuity, challenges in the pursuit of research related to olfactory receptors (ORs) continue to exist.

Grown exponentially by the genomic revolution, the use of the rat as a model of choice for physiological studies continues in popularity and at a much greater depth of understanding.

Significant advancements have been made in the study of chromatin structure and function over the past fifty years but none as spectacular as those made in the last decade due to the development of novel techniques and the ability to sequence large stretches of DNA.

Plant genomics is a growing and constantly evolving field of study, one which has gained much ground in past years through the development of advanced research and data management tools.

The generation of genetically modified mice is absolutely crucial to gene function studies today, primarily because mice are genetically similar to man and because gene function studies in mice are in the context of a whole organism, making them particularly useful.

Gene regulatory networks play a vital role in organismal development and function by controlling gene expression.

Since the discovery of DNA structure and throughout the ensuing "e;DNA era"e;, the field of DNA replication has expanded to cover a vast number of experimental systems.

Featuring a diverse array of model organisms and scientific techniques, Sirtuins: Methods and Protocols collects detailed contributions from experts in the field addressing this vital family of genes.

Recent efforts to characterize genetic variation in the human genome, coupled with the rapidly developing field of genomics, have lead directly to the development of new and innovative approaches to the identification of genes contributing to complex human diseases.

This book addresses cutting-edge techniques for researching transposon mutagenesis, an approach for identifying individual gene contributions to the phenotypic characteristics of a particular microorganism.

During the 20th century Xenopus has flourished as an accessible experimental model system for studying vertebrate biology.

This volume provides readers with a comprehensive review of various gene silencing methodologies and its applications.

Genetic approaches to understanding plant growth and development have always benefitted from screens that are simple, quantitative and rapid.

With the sequencing of the human genome complete, the field of proteomics, still in its early stages, has become an important and informative field of biomedical research.

Only in recent times has the possibility of growing and implanting replacement teeth, made from one's own cells, moved into the realm of realistic possibilities; however, the molecular and cellular mechanisms of tooth development must be studied in a range of vertebrates, from zebrafish to mice, so that evolutionarily conserved network kernels, which will define the cellular states of generic vertebrate tooth development, can be recognized.