Rapid advances in our understanding of basic cell biological processes and of the molecular mechanisms of cell function and dysfunction have led to an increasing interest in utilizing these approaches in neurobiological research.
to the Animal Models Volumes This and several other volumes in the Neuromethods series will describe a number of animal models of neuropsychiatric disorders.
The need to better understand the molecular, b- chemical, and cellular processes by which a developing neuronal system unfolds has led to the development of a unique set of experimental tools and organisms.
This volume collects protocols and procedures utilizing cellular, tissue, and whole animal models that can be applied to the investigation of neurotrophic factors and other agents impacting on these systems.
While researchers with Positron Emission Tomography (PET) and Single Photon Emission Computed Tomography (SPECT) essentially addressed questions from the whole spectrum of cardiology, oncology, and the neurosciences, it was most notably the latter that provided completely new insights into physiological and disturbed human brain function.
This volume discusses the importance of imaging, fluid, and genetic biomarkers in detecting the earliest pre-symptomatic stages of Alzheimer's disease (AD).
Bringing together techniques and methods currently being applied to the study of exocytosis, Exocytosis Methods collects chapters from experts in the field, examining this fundamental process essential to functions ranging from protein secretion to hormone release and neurotransmission.
Societal, ethical, and cost-related issues, not to mention the need for sound scientific methods, have led to new and refined methods for the evaluation of health risks associated with neurotoxic compounds, relevant and predictive of exposure, relatively inexpensive, and ideally amenable to high throughput analysis and a reduction in animal use.
Affecting over a hundred million individuals worldwide, retinal diseases are among the leading causes of irreversible visual impairment and blindness, and appropriate study models, especially animal models, are essential to furthering our understanding of the etiology, pathology, and progression of these endemic diseases.
As a model organism, zebrafish (Danio rerio) offer an opportunity to perform steadfast scientific investigations in a robust and high-throughput manner.
For molecules that are not directly electroactive, it is necessary to modify a microelectrode by grafting polymeric or enzymatic membranes capable of translating a local concentration into an electrical current, which is a concept referred to as biosensing.
The growth of the field of eating disorder research has led to a vast array of empirical articles, and the development of new animal models that can be used to study these disorders continues to stimulate new research.
This book explores several aspects of the research in the field of general anesthesia, including mechanisms of action of inhaled and intravenous hypnotic agents; the anesthetics-induced neurotoxicity in children and elderly; features and physiopathology of postoperative cognitive side effects; and findings from preclinical research focused on the recognition of potential links between anesthetics and cancer cell biology.
Reflecting a vast amount of new information concerning the functional characteristics of the various 5-HT receptor subtypes and the Na+-dependent serotonin transporter (SERT), this volume provides state of the art methodologies currently applied in serotonin research from leading experts in the field.
This volume provides methods for characterization of muscarinic receptor in crystallography studies that advanced our understanding of structural properties and activation mechanism of muscarinic receptors and are cornerstone in molecular modeling and computer based approaches to study muscarinic receptors.
This detailed volume compiles the best methodologies and experimental techniques to profile and extract maximal data from brain tumor stem cells (BTSCs), the experimental paradigm for brain cancer research that offers insights into cancer stem cell populations that may drive not only tumor initiation but tumor recurrence and patient relapse.
Applying neurophysiological methods to the study of brain-behavior relationships proved to be a major advance in the early days of neuroscience research.
This volume describes a broad spectrum of experimental approaches for investigating structure, function, and transport of neuronal mitochondria in health and disease.
This volume provides the reader with detailed protocols that describe a variety of in vitro and in vivo techniques that study reductionist systems and human samples.
With the loss of work days, the price of health care and payments for compensation, litigation, and malpractice, and the overwhelming cost of human suffering, chronic pain syndromes affect humanity enormously on both an economic and personal level.
In Neuronal Cell Culture: Methods and Protocols, the latest aspects of the culture of neural cells are explored by experts in the field who also explain the practical and theoretical considerations of the techniques involved.
This volume details protocols on prion disease from multiple disciplines and highlights the contribution each discipline has made to the understanding of prion disease.
This volume details various technological advances to isolate, perpetuate, and characterize neural stem cells from various sources and formulates them for the delivery into animal models of neurological disorders.
Preclinical research related to mood and anxiety disorders relies extensively upon mouse behavioral tests and models, the use of which continues to increase as a greater number of underlying susceptibility genes are discovered, new targets for medications are identified, and clinical studies reveal novel neurobiological risk factors.
This volume provides in-depth descriptions and discussion of clinical trial designs based on novel data analysis of high-quality trials for 11 different neurological conditions in order to ultimately improve the quality of care in neurology.
Despite the difficulty in comparing clinic-based human tests with animal model testing, there is still great value in pursuing translational approaches, as tests and treatment strategies might be developed to improve brain function in humans suffering from neurological conditions and knowledge obtained from human behavioral studies can be used to further improve the animal models of behavioral analysis.
This volume provides comprehensive knowledge on the fundamental techniques and detailed laboratory protocols used in stem cell research and their potential application in the field of neuroscience.
Evolving technologies starting with tracer studies, and more recently with genomics and proteomics, have provided novel information about the molecular properties of cerebral endothelium and astrocytes; however, further studies must be done in animal models of neurological diseases and in humans to get a clearer understanding of the pathogenesis of blood-brain barrier (BBB) breakdown in nervous system diseases.
This volume highlights proteomics studies of quantitative PTM changes in both peripheral and central nervous system proteomes utilizing the most recent advances in mass spectrometry.
This volume focuses on experimental research with applicable models to study physiology, biochemistry, and molecular biology of the blood-brain barrier (BBB).
Although there has been an explosion of interest and technology in the study of neural stem cells, many questions related to stem cell properties and neural stem cell lineage and differentiation still linger.
Min Li and a panel of hands-on experimentalists detail state-of-the-art molecular techniques for studying NMDA ligand-gated ion channels and developing assays for nontherapeutic lead selection.
