With the significant medical role that human cytomegalovirus plays in human disease (from acute disease following primary infection, to chronic disease due to life-long viral persistence), the need to better understand human cytomegalovirus biology and pathogenesis is needed.
Neisseria meningitidis is a major causative agent, worldwide, of potentially life-threatening meningitis and septicaemia, which carry with them a high mortality rate and permanent physical and neurological issues for survivors.
Horizontal gene transfer (HGT) events encompass processes as varied as the exchange of genetic material between microbes coexisting in the same environment, between symbiotic bacteria and their eukaryotic hosts, and the evolution of organelles by symbiosis, in which whole genomes are acquired.
Filamentous fungi have long been known for their ability to produce an enormous range of unusual chemical compounds known as secondary metabolites, many of which have potentially useful antibiotic or pharmacological properties.
Following the considerable success of the first edition of Plant Virology Protocols, this exciting new edition covers the many new techniques that are now applied to the examination and understanding of plant viruses.
Since its introduction almost 20 years ago, phage display technology has revolutionized approaches to the analysis of biomedical problems, quickly impacting the fields of immunology, cell biology, biotechnology, pharmacology, and drug discovery.
Since the publication of the popular first edition, genomic methods have become more accessible, allowing antibiotic researchers to probe not only the sequence of antibiotic resistance determinants but the mechanism whereby they are expressed and regulated.
Baculovirus Expression Protocols is a detailed guide for using the baculovirus expression vector system (BEVS) and/or insect cells to produce recombinant proteins.
PCR methods for the detection of microbial pathogens have made relatively little impact in diagnostic microbiology laboratories due to the common decision to use expensive commercially produced tests rather than the cheaper alternative of developing one's own tests or introducing tests developed by other workers.
An up-to-date view of molecular mechanisms for investigating microbial communities and their biological activities, this new volume of Environmental Microbiology: Methods and Protocols looks at recent advances that are having a big impact on the field such as metagenomics and other "e;omics"e; technologies, NanoSIMS, as well as stable isotope probing and more.
Reflecting the development of powerful new tools and high-throughput methods to analyze adenoviral particles and their interactions with host cells, the third edition of Adenovirus Methods and Protocols calls upon experts in the field to convey advances in molecular biology, genomics and proteomics, imaging, and bioinformatics.
This book, split into two volumes, presents a broad coverage of the principles and recent developments of sample preparation and fractionation tools in Expression Proteomics in general and for two-dimensional electrophoresis (2-DE) in particular.
Systems Biology aims at deciphering the genotype-phenotype relationships at the levels of genes, transcripts (RNAs), peptides, proteins, metabolites, and environmental factors participating in complex cellular networks in order to reveal the mechanisms and principles governing the behavior of complex biological systems.
Each generation in a sexually reproducing organism such as a fly or a mouse passes through the bottleneck of meiosis, which is the specialized cell division that gives rise to haploid reproductive cells (sperm, eggs, spores, etc.
Identifying Helicobacter infection as the leading cause of peptic ulcer disease and gastric cancer has dramatically altered the treatment of these disease states.
Each generation in a sexually reproducing organism such as a fly or a mouse passes through the bottleneck of meiosis, which is the specialized cell division that gives rise to haploid reproductive cells (sperm, eggs, spores, etc.
Due to the highly collaborative nature of investigators working in the field, we have rapidly advanced our understanding of Staphylococcus epidermidis and other staphylococci in the last two decades.
Metabolic engineering is the practice of genetically optimizing metabolic and regulatory networks within cells to increase production and/or recovery of certain substance from cells.
Adenovirus Methods and Protocols, Second Edition, now in two volumes, is an essential resource for adenovirus (Ad) researchers beginning in the field, and an inspirational starting point for researchers looking to branch into new areas of Ad study.
Sexually transmitted infections (STI) continue to be a major cause of morbidity and mortality, both in developed industrial countries as well as in the developing world.
In recent years, molecular microbiology has emerged as a top, cutting-edge biological discipline, thanks to the multi-disciplinary and integrative approaches taken by investigators seeking to understand the intricacies of the microbial world and how it affects human health and the biosphere.
Structural genomics is a newly emerging field that has arisen following the successful footsteps of the major sequencing efforts generally bundled under the heading "e;genomics"e;.
Ranging from the evolution of pathogenicity to oceanic carbon cycling, the many and varied roles that bacteriophages play in microbial ecology and evolution have inspired increased interest within the scientific community.
Despite major advances in HIV treatment, many areas require more study, in order to create efficacious, potent antiretrovirals that can suppress viral load completely and durably without toxic side effects, to define unknown drug targets and fine-tune known targets, and to better understand the interplay between viral and host factors.
Leading experts in enzyme manipulation describe in detail their cutting-edge techniques for the screening, evolution, production, immobilization, and application of enzymes.
The development of biotechnology over the last 20 years, and particularly the use of recombinant DNA techniques, has rapidly expanded the opportu- ties for human benefits from living resources.
A collection of readily reproducible classic and emerging molecular methods for the laboratory isolation and identification of the pathogens, viruses, and parasites that cause food-borne disease.
Metagenomics has proven to be a powerful tool for exploring the ecology, metabolic profiling, and comparison of complex microbial communities as well as its important applications in the mining of metagenomes for genes encoding novel biocatalysts and drug molecules for bioindustries.
Systems biology is the study of interactions between assorted components of biological systems with the aim of acquiring new insights into how organisms function and respond to different stimuli.
Due to their novel concepts and extraordinary high-throughput sequencing capacity, the "e;next generation sequencing"e; methods allow scientists to grasp system-wide landscapes of the complex molecular events taking place in various biological systems, including microorganisms and microbial communities.
